Improving the SAR Distribution in Petri-Dish Cell Cultures

Angulo, Luis D.; Garcia, S.G.; Pantoja, Mario F.; Sánchez, Clemente Cobos; Martin, R.G.

doi:10.1163/156939310791036322

Cited by 14 publications

(17 citation statements)

References 16 publications

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“…It is known that, at the same incident E-field strength, electromagnetic energy absorption in objects is dictated by their dielectric parameters and by their geometry [2,24,25]. Techniques for determining complex permittivity dispersion of any materials exist [26]; based on this, we chose here a set of four liquids for the dosimetric approach.…”

Section: Dosimetric Validation Of the Tem Cellmentioning

confidence: 99%

Design and Validation of a Tem Cell Used for Radiofrequency Dosimetric Studies

Iftode¹,

Miclăuș²

2012

PIER

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Abstract-A Transverse Electromagnetic Mode (TEM) cell is an interesting option for studying the biological effects of radiofrequency radiation at reduced scale (in vitro studies).Controlled and well-characterized exposure conditions are essential for a conclusive investigation: the biological sample is exposed to a uniform incident electromagnetic wave and the dose of absorbed radiation is precisely determined and correlated with the effect. Unfortunately, experimental dosimetry is often unavailable or inapplicable, so that a precharacterized and validated experimental setup is most valuable. As such, the primary objective of the present work is to experimentally validate a computational model of a self-built TEM cell designed for bioelectromagnetic experiments in the 100 MHz-1 GHz frequency range. Validation is achieved by comparing the computed vs. measured values for three significant parameters: scattering parameters, incident electric field distribution, and absorbed power in a set of liquid samples. Successful validation and characterization is achieved by using CST Microwave Studio's finite integration technique (FIT), and respectively a network analyzer for the experimental setup. The secondary objective is a dosimetric study of four different liquid samples loaded in the cell. The absorption coefficient (AC) is used, assimilated to the specific absorption rate (SAR) of energy deposition in the entire sample volume. AC is shown to converge in experiment and simulation up to 800 MHz for all samples. AC doesn't depend directly on the samples' volume (despite greater volumes frequently showing higher absorption) but rather upon the internal field distribution, which in turn mostly depends on the frequency and on the dimensions of the liquid samples.

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Section: Dosimetric Validation Of the Tem Cellmentioning

confidence: 99%

Design and Validation of a Tem Cell Used for Radiofrequency Dosimetric Studies

Iftode¹,

Miclăuș²

2012

PIER

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“…To induce and control the growth of these cell cultures, they may be exposed to various electromagnetic (EM) fields. One common scenario is to place the Petri dish into a rectangular waveguide that is illuminated with a polarized wave radiating at a particular frequency, [1,18,17].…”

Section: Introductionmentioning

confidence: 99%

“…In order to ensure the proper growth of the cell cultures, it is necessary to guarantee the high and uniform distribution of the EM energy (typically measured in terms of SAR -Specific Absorption Rate-) within the Petri dish [1,20]. Some authors state that in addition to control the SAR, one also needs to impose some additional conditions, e.g., polarization, on the distribution of the full electromagnetic fields to secure the proper evolution of the cell culture (see [22] and references therein).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, it should provide an error estimation in order to minimize uncertainty and guarantee the correctness of the solution for each model. Several numerical methods have been employed for Petri dish simulations exposed to EM fields in different configurations, e.g., [3,20,21,16,1,22,23]. Approaches based on differential formulations are mainly used because of their flexibility to deal with complex geometrical and material configurations.…”

Section: Introductionmentioning

confidence: 99%

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High-accuracy adaptive modeling of the energy distribution of a meniscus-shaped cell culture in a Petri dish

Gomez-Revuelto

Garcı́a-Castillo

Pardo

2015

Journal of Computational Science

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Cylindrical Petri dishes embedded in a rectangular waveguide and exposed to a polarized electromagnetic wave are often used to grow cell cultures. To guarantee the success of these cultures, it is necessary to enforce that the specific absorption rate distribution is sufficiently high and uniform over the Petri dish. Accurate numerical simulations are needed to design such systems. These simulations constitute a challenge due to the strong discontinuity of electromagnetic material properties involved, the relative low field value within the dish cultures compared with the rest of the domain, and the presence of the meniscus shape developed at the liquid boundary. The latter greatly increases the level of complexity of the model in terms of geometry and intensity of the gradients/singularities of the field solution. In here, we employ a three-dimensional (3D) hp-adaptive finite element method using isoparametric elements to obtain highly accurate simulations. We analyze the impact of the geometrical modeling of the meniscus shape cell culture in the hp-adaptivity. Numerical results showing the error convergence history indicate the numerical difficulties arisen due to the presence of a meniscus-shaped object. At the same time, the resulting energy distribution shows that to consider such meniscus shape is essential to guarantee the success of the cell culture from the biological point of view.

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“…The thermal reaction and its mechanism of sterilization using electromagnetic wave have been widely accepted [6][7][8][9]. The non-thermal effect has been a research hotspot in recent years [10]. The electric fields of nanosecond duration and above 10 kV/cm amplitude can affect the subcellular structures which can lead to the apoptosis of cells, but do not lead to formation of pores in the outer membrane.…”

Section: Introductionmentioning

confidence: 99%

A 2.45 GHZ Reentarnt Coaxial Cavity for Liguid Sterilization Based on Non-Thermal Microwave Effect

Zhang¹,

Zhang

Zhang³

2012

PIER C

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Abstract-According to the intracellular electromanipulation model, bacteria can be killed at as high as 10 6 V/m electrical fields on microwave band. We designed and constructed a modified microwave coaxial cavity resonator for liquid sterilization. The cavity could concentrate the field on a very small area, and the liquid can pass it in milliseconds. The bacteria can be killed by the very high field, but with a slight temperature increase. The designed resonator is simulated and analyzed by electromagnetic simulation code. The results indicated that when the input power reached 100 W, the electric field on the area of liquid can reach 10 6 V/m. Preliminary experimental results indicated that when the input power was 100 W, the bactericidal rate was > 90%, and the temperature of the liquid only increased 8.6 • C.T M

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Improving the SAR Distribution in Petri-Dish Cell Cultures

Cited by 14 publications

References 16 publications

Design and Validation of a Tem Cell Used for Radiofrequency Dosimetric Studies

Design and Validation of a Tem Cell Used for Radiofrequency Dosimetric Studies

High-accuracy adaptive modeling of the energy distribution of a meniscus-shaped cell culture in a Petri dish

A 2.45 GHZ Reentarnt Coaxial Cavity for Liguid Sterilization Based on Non-Thermal Microwave Effect

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