Improving the attachment and proliferation of umbilical cord mesenchymal stem cells on modified polystyrene by nitrogen-containing plasma

Abstract

Wharton's jelly mesenchymal stem cells (WJMSCs) are important alternative source of pluripotent cells for several therapeutic purposes. Understanding of adhesion properties of such cells is necessary to regulate the attachment, growth and proliferation on targeted culture surfaces. BCP-K1, a line of WJMSCs, and polystyrene (PS) culture dishes were used as membrane samples. A 13.56 MHz inductively coupled discharge plasma reactor with a mixture of N-containing gas and noble gas was used. This was expected to introduce the more hydrophilic groups on PS surface and enhance the cell adhesion. The plasma-treated PS dishes with the mixed gas of N(2) + He at 50 W and NH(3) + He at 100 W were reactive towards BCP-K1. Cellular adhesion and proliferation was significantly twice as efficient on the treated surfaces than on PS dishes. BCP-K1 also secreted more focal adhesion kinase to adhere and proliferate when cultured on N(2)-treated PS dishes than on the NH(3)-treated PS dishes. Stable stemness markers were detected, including CD105, CD9 and SSEA-4, expressed on BCP-K1 growing on the modified PS dish surfaces, during 7 days of culturing. The presence of -NH(2) groups on the PS dish surface were revealed by X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. A large amount of oxygen- and nitrogen-containing functional groups, up to 9.0 %, were introduced by NH(3) plasma and N(2) plasma. The functional groups introduced on to the PS surfaces were clearly the key factors which enhanced WJMSCs attachment and stemness stability.

Biocompatibility Assessment of PLCL‐Sericin Copolymer Membranes Using Wharton’s Jelly Mesenchymal Stem Cells

“…Initial cell attachment plays an important role in further cell viability on biomaterials; thus the pattern of architecture and surface properties of the materials are crucial [ 29 ]. Although most of the observed WJMSC of this study appeared as separated, single, attached cells, some colonies were still found on the surfaces.…”

“…BCP-K1 (hWJMSC line) was obtained from the Human and Animal Cell Technology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Thailand. The cells were cultured in a complete medium, supplemented with 10% (w/v) fetal bovine serum and essential growth factors and maintained as described in Tunma et al [ 29 ]. Briefly, the medium was replaced every 2 days; trypsinization was performed when cell density reached around 70–80% of the culture surface.…”

Chitin and carbon nanotube composites as biocompatible scaffolds for neuron growth

“…Plasma treatment was shown to be a very effective method to increase adhesion of different cell types on various biomaterials 40,41 . Oxygen plasma treatment is commonly used to introduce hydrophilic surfaces onto fabricated polymers.…”

Near-Infrared, Light-Triggered, On-Demand Anti-Inflammatories and Antibiotics Release by Graphene Oxide/Elecrospun PCL Patch for Wound Healing

“…In fact, corrosion of the polymeric surface could occur, and this might affect the 3D macrostructure of the PCL scaffold. This approach has been successfully used for scaffold engineering to provide functionalization of the surface with primary amines, even if keeping bulk properties of the virgin polymeric material, and also to enhance cell adhesion properties of hydrophobic polymers [22,[28][29][30]. Furthermore, primary amines also allow monolayer covalent deposition of GO through the reaction with epoxy groups intrinsically contained in GO sheets [31].…”