Biocompatibility Assessment of PLCL‐Sericin Copolymer Membranes Using Wharton’s Jelly Mesenchymal Stem Cells

Abstract

Stem cells based tissue engineering requires biocompatible materials, which allow the cells to adhere, expand, and differentiate in a large scale. An ideal biomaterial for clinical application should be free from mammalian products which cause immune reactivities and pathogen infections. We invented a novel biodegradable poly(L-lactic-co-ε-caprolactone)-sericin (PLCL-SC) copolymer membrane which was fabricated by electrospinning. Membranes with concentrations of 2.5 or 5% (w/v) SC exhibited qualified texture characteristics with a noncytotoxic release profile. The hydrophilic properties of the membranes were 35–40% higher than those of a standard PLCL and commercial polystyrene (PS). The improved characteristics of the membranes were due to an addition of new functional amide groups, C=O, N–H, and C–N, onto their surfaces. Degradation of the membranes was controllable, depending on the content proportion of SC. Results of thermogram indicated the superior stability and crystallinity of the membranes. These membranes enhanced human Wharton's jelly mesenchymal stem cells (hWJMSC) proliferation by increasing cyclin A and also promoted cell adhesion by upregulating focal adhesion kinase (FAK). On the membranes, hWJMSC differentiated into a neuronal lineage with the occurrence of nestin. These data suggest that PLCL-SC electrospun membrane represents some properties which will be useful for tissue engineering and medical applications.

Effects of plasma treatment on biocompatibility of poly[(L-lactide)-co -(ϵ -caprolactone)] and poly[(L-lactide)-co -glycolide] electrospun nanofibrous membranes

“…The ability of electrospun membranes to support the growth of various cell lineages, including Schwann cells, embryonic stem cells, neural stem cells and mesenchymal stem cells, has been reported . Olfactory ensheathing cells (OECs) are considered as peripheral nerve progenitor cells which develop from a peripheral origin .…”

Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds

“…These results suggest that PLC is a flexible substrate for multiple applications in regenerative medicine and tissue engineering. The viability of attached cells, an important factor, definitely indicates the cytotoxicity and biocompatibility of novel materials [20,22]. Previous studies showed a non-cytotoxicity of PCL scaffolds either in 2D or 3D forms by maintenance proliferation and functions of various cell types, such as porcine chondrocytes [19], rat hippocampus [23], human bone marrow mesenchymal stem cells [24], and mouse embryonic stem cells [1].…”

“…Focal adhesion kinase (FAK) and cyclin-A protein expression levels were used as markers for cell proliferation and attachment, respectively. The assays were performed as described by Inthanon et al (2016) [20]. Briefly, cells were fixed in 4% (v/v) formaldehyde for 30 min at room temperature and then washed three times (5 min/wash) with ice-cold washing buffer (0.05% (v/v) Tween 20 in 1X PBS), with gentle rocking.…”

Growth and Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cells on oxygen Plasma-Modified 2D and 3D Polycaprolactone Scaffolds

“…proliferation and attachment, respectively. The assays were performed as described by Inthanon et al (2016) [11]. Briefly, cells were fixed in 4% (v/v) formaldehyde for 30 min at room temperature and then washed three times (5 min/wash) with ice-cold washing buffer (0.05% (v/v) Tween 20 in 1X PBS) with gentle rocking.…”