Improving Osteogenesis Activity on BMP-2-Immobilized PCL Fibers Modified by theγ-Ray Irradiation Technique

Abstract

The purpose of this study was to demonstrate the ability of BMP-2-immobilized polycaprolactone (PCL) fibers modified using the γ-ray irradiation technique to induce the osteogenic differentiation of MG-63 cells. Poly acrylic acid (AAc) was grafted onto the PCL fibers by the γ-ray irradiation technique. BMP-2 was then subsequently immobilized onto the AAc-PCL fibers (BMP-2/AAc-PCL). PCL and surface-modified PCL fibers was characterized by evaluation with a scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. The biological activity of the PCL and surface-modified PCL fibers were characterized by alkaline phosphatase (ALP) activity, calcium deposition, and the mRNA expression of osteocalcin and osteopontin in MG-63 cells. Successfully grafted AAc and PCL fibers with immobilized BMP-2 were confirmed by XPS results. The results of the contact angle showed that BMP-2/AAc-PCL fibers have more hydrophilic properties in comparison to PCL fibers. The ALP activity, calcium deposition, and gene expressions of MG-63 cells grown on BMP-2/AAc-PCL fibers showed greatly induced osteogenic differentiation in comparison to the PCL fibers. In conclusion, these results demonstrated that BMP-2/AAc-PCL fibers have the potential to effectively induce the osteogenic differentiation of MG-63 cells.

Functionalization of PCL-3D electrospun nanofibrous scaffolds for improved BMP2-induced bone formation

“…Water contact angle was also noticeably decreased, with lowered hydrophobicity of scaffolds possibly playing into increased osteogenic differentiation of cells on PCL/HA-3d scaffolds. Consistently, more intense cytoskeletal staining was observed on the HA-modified scaffolds, suggesting stronger cell attachment on PCL/HA-3D scaffolds possibly contribute to the improved osteogenic differentiation as well [34, 35].…”

A New Polycaprolactone-Based Biomembrane Functionalized with BMP-2 and Stem Cells Improves Maxillary Bone Regeneration

“…Kim et al [ 34 ] observed that the release of BMP-2 from their PCL-based scaffold resulted in a remarkable increase in new bone formation. Lee et al [ 35 ] and Yun et al [ 36 ] have shown that the release of BMP-2 leads to an increase in alkaline phosphatase (ALP) activity, ALP being an early osteogenic marker. The release of BMP-2 by their PCL scaffold promotes the differentiation of cells into bone lineage cells.…”

Enhanced osteogenesis on biofunctionalized poly(ɛ-caprolactone)/poly(m-anthranilic acid) nanofibers

“…These results indicated that BMP-2 was successfully immobilized onto the surface of PCL/ P3ANA nanofibers. 24…”

“…3,19,22,23 Carboxylic acid groups of P3ANA not only provide active sides for covalent BMP-2 immobilization onto the nanofibers but also result hydrophilic surface which enhances the cellnanofiber interactions. 24 PCL which is a biocompatible and biodegradable polymer was used to obtain electrospun nanofibers of P3ANA. 25 PCL have been used especially in bone tissue engineering due to its slow degradation nature.…”