Improving of rooting and ex vitro acclimatization phase of Agave tequilana by temporary immersion system (BioMINT™)

Monja-Mio, Kelly M.; Olvera-Casanova, Diego; Herrera-Herrera, Gastón; Herrera-Alamillo, Miguel Ángel; Teyer, Lorenzo Felipe Sánchez; Robert, Manuel L.

doi:10.1007/s11627-020-10109-5

Cited by 23 publications

(10 citation statements)

References 48 publications

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“…Ahmadian et al [ 35 ] in carnation ( Dianthus caryophyllus L.) achieved 90% survival using the TIB. Monja-Mio et al [ 36 ] in agave ( Agave tequilana Weber var. Azul) increased survival up to 96% in a greenhouse when using temporary immersion.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Mancilla-Álvarez

Pérez-Sato

Núñez-Pastrana

et al. 2021

Plants

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Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

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Section: Discussionmentioning

confidence: 99%

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Mancilla-Álvarez

Pérez-Sato

Núñez-Pastrana

et al. 2021

Plants

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“…Domínguez Rosales et al (2008) report that, after 30-45 days of acclimatization in A. potatorum seedlings, 73% of the seedlings survived. Monja-Mio et al (2020) reported 73% survival of A. tequilana seedlings.…”

Section: Seedling Acclimatizationmentioning

confidence: 99%

Cost analysis of Agave potatorum Zucc, produced in vitro by direct organogenesis

VELAZQUEZ

Pérez-de-León

Magaña

et al. 2022

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Objective: To demonstrate the efficiency and profitability of temporary immersion bioreactors compared to propagation in gelled media.Design/Methodology/Approach: Agave potatorum seedlings were introduced to the in vitro system in order to compare their productivity, multiplication rate, and propagation time. An investment project was carried out considering the equipment depreciation, but without considering the construction of the property. Based onthese premises, the cost of production per plant was evaluated with a goal of 500,000 seedlings in mind.Results: A 3% contamination was recorded when the in vitro system was first introduced. The semi-solid multiplication rate was 4 shoots per explant and 18 shoots per explant in bioreactors, both at 30 days of incubation. A 10% handling loss was taken into consideration. The production cost was US$0.16 (MNX$3.20) for gelled media and US$0.09 (MNX$1.80) for propagation in temporary immersion bioreactors. The Internal Rates of Return for gelled media and for propagation with bioreactors were 2.33 and 3.75, respectively.Study Limitations/Implications: The study does not take into consideration theconstruction of the property, although it does consider equipment depreciation.Findings/Conclusions: Thanks to scientifical and technological development, the use of biotechnological tools is becoming more profitable every day. These technologies are already available to be transferred and this kind of research demonstrates its profitability, highlighting the potential establishment of technology- based companies.

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“…If the plant material is not vigorous, abnormal stomatal development, absence of epicuticular waxes and few functional roots may occur. An effective micropropagation protocol is essential to achieve high levels of survival (Rescalvo-Morales et al 2019;Monja-Mio et al 2020).…”

Section: Determination Of Viability and Phytosanitary Quality Of In V...mentioning

confidence: 99%

Development of control strategies for bacteria and fungi associated with a micropropagated new cultivar of orange-fleshed sweet potato (Ipomoea batatas cv. Agrosavia–Aurora)

Pérez-Pazos

Rosero

Cardinale

et al. 2023

Hortic. Environ. Biotechnol.

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Vegetative propagation of sweet potato lead to the accumulation of diseases from generation to generation, which represents a threat to both productivity and conservation of genetic resources. In vitro techniques can help to overcome phytosanitary problems by applying plant material cleaning strategies. The objective of this study was to develop in vitro micropropagation strategies for the production of high-quality plant material of an orange-fleshed variety of sweet potato recently released in Colombia. Molecular identification of contaminating microorganisms was performed by sequencing the 16S rRNA gene for bacteria and ITS for fungi. Five disinfection protocols were evaluated, three of which were previously developed for sweet potato and included disinfection with 0.5, 1, and 2% sodium hypochlorite respectively, while two protocols are proposed in this work and included washing with povidone-iodine, disinfection with sodium hypochlorite 2%; one of these two new protocols also contains acetic acid and quaternary ammonium. For the evaluation of the viability of in vitro plants after disinfection, they were acclimatized in a greenhouse, reintroduced, and a molecular testing by PCR of 16S rRNA gene and ITS was carried out to verify the phytosanitary status of the material. The contaminating microorganisms found were filamentous fungi of the genera Fusarium, Sarocladium, Cladosporium and Aspergillus, yeasts of the genera Pseudozyma and Moesziomyces, and the actinobacterium Curtobacterium sp. The results indicated that washing with povidone-iodine and disinfection with 2% sodium hypochlorite, acetic acid and quaternary ammonium was the most efficient disinfection protocol, reducing the number of contaminated cultures by up to 10% and eradicating 70% of contaminants. The in vitro plants established in the greenhouse remained healthy and, after reintroduction, the molecular test for bacteria and fungi was negative. These results allowed the generation of an optimized protocol that can be incorporated into the in vitro micropropagation process to generate contamination-free sweet potato seeds.

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Improving of rooting and ex vitro acclimatization phase of Agave tequilana by temporary immersion system (BioMINT™)

Cited by 23 publications

References 48 publications

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Cost analysis of Agave potatorum Zucc, produced in vitro by direct organogenesis

Development of control strategies for bacteria and fungi associated with a micropropagated new cultivar of orange-fleshed sweet potato (Ipomoea batatas cv. Agrosavia–Aurora)

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