Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Mancilla-Álvarez, Eucario; Pérez-Sato, Juan Antonio; Núñez-Pastrana, Rosalía; Spinoso-Castillo, José Luis; Bello-Bello, Jericó Jabín

doi:10.3390/plants10051010

Cited by 13 publications

(5 citation statements)

References 38 publications

(55 reference statements)

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“…From the results of the present study, a similar positive effect on light acquisition with the use of the ElecTIS bioreactor can be hypothesized. In accordance, the minor chlorophyll and carotenoid amounts from shoots cultured in glass jars with gelled media, observed also in blackberry, was reported by Mancilla-Álvarez et al [24], who hypothesized that the culture in climatic chambers with classic glass jars, positioned vertically on multiple levels, limits the amount of light the explants receive. However, a medium effect (liquid or gelled) on these parameters cannot be excluded.…”

Section: Discussionsupporting

confidence: 82%

“…The results in this study demonstrate the efficiency of the ElecTIS bioreactor in promoting the shoot growth and proliferation of blackberry shoots coming from SGS at a low temperature (4 • C) and in darkness, in comparison with the gelled medium. In recent years, the mass production of micropropagated plants, based on the use of liquid culture in TIS bioreactors, has been established and developed for a wide range of species, e.g., banana [22], myrabolan rootstock [23], taro [24,25] and stevia [26]. Mancilla-Álvarez et al [27] suggested that the assimilation of sugars, nutrients and growth regulators from the liquid culture medium when in contact with the entire surface of the explant could be an explanation for the increase in the multiplication rate of Colocasia esculenta, while the lower availability of organic and inorganic compounds in the gelled medium, due to the limited contact of shoots at their base, determines the lower multiplication rates.…”

Section: Discussionmentioning

confidence: 99%

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Use of Liquid Culture with the ElecTIS Bioreactor for Faster Recovery of Blackberry (Rubus fruticosus L.) Shoots from Conservation at 4 °C

et al. 2023

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ElecTIS is a new single container bioreactor which does not require forced air blowing, instead making the culture material mobile and the liquid medium stationary. The timed up-and-down movement of the basket containing the shoot culture ensures periodic contact with the liquid medium positioned at the base of the container. In this study we tested for the first time its use in the recovery of blackberry shoot cultures (Rubus fruticosus L., cvs Thornfree and Chester), coming from 5 months of slow growth storage (SGS), at 4 °C and in the dark. The shoot recovery at standard culture conditions was performed on two different types of ElecTIS, i.e., one with a smaller basket (ElecTISS, 234 cm2 of culture area), and one with a large basket (ElecTISL, 336 cm2), comparing the culture in TIS (cycle of 8 min every 6 h, equal to 32 min/day) with the traditional one in a gelled medium in glass jars (500 cc). After each one of the three 4-week subcultures, the shoot growth parameters and the relative growth rate highlighted a clear superiority of ElecTIS in promoting the recovery of shoot cultures coming from SGS. The analyses of chlorophyll content and stoma functionality confirmed the superior quality of shoots cultured in the ElecTIS bioreactor, and these shoots were afterwards easily rooted and acclimatized ex vitro.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Use of Liquid Culture with the ElecTIS Bioreactor for Faster Recovery of Blackberry (Rubus fruticosus L.) Shoots from Conservation at 4 °C

et al. 2023

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“…The control group was significantly higher than all other treatments. Irradiance is an important factor in physiological processes that affects the synthesis of chlorophyll [26], leading to potentially two main reasons for our results:…”

Section: Discussionmentioning

confidence: 99%

Micropropagation and Acclimatization of Monstera deliciosa Liebm. ‘Thai Constellation’

Jing,

Beleski,

Vendrame

2023

Horticulturae

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Monstera deliciosa Liebm. ‘Thai Constellation’ is a variegated variety of M. deliciosa belonging to the family Araceae, which has become a new favorite in the foliage plant market. However, limited studies exist on its propagation, and growers have difficulties in achieving large-scale production. This study aimed at developing an efficient protocol for the micropropagation of M. deliciosa using SETIS™ temporary immersion bioreactors. Furthermore, we aimed at evaluating the role of a novel biostimulant (IQ Forte) in the acclimatization of M. deliciosa. Significant differences were observed among the different treatments, showing higher multiplication rates under TIS conditions as compared to the semi-solid control. Adjusting immersion parameters also showed benefits in improving multiplication rates. The novel biostimulant (IQ Forte) did not provide significant gains in growth of M. deliciosa ‘Thai Constellation’ during acclimatization.

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“…Yoon et al [44] revealed that biomass production from Anoectochilus formosanus was less efficient when culturing was performed under temporary immersion. In a recent study on in vitro multiplication of Colocasia esculenta, applying different culture systems demonstrated that the highest regeneration rate was with TIS [57].…”

Section: Culture System and Biomass Productionmentioning

confidence: 99%

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

et al. 2021

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The cultivation of medicinal plants and the production of bioactive compounds derived from them are of fundamental importance and interest, not only at the pharmacological level but also in nutraceutical and cosmetic industries and in functional foods, as well as plant protection in agriculture. In order to respond adequately to the increased demands of the global market from a quantitative and qualitative point of view and to guarantee environmental sustainability of the productions, it is necessary to resort to innovation tools, such as tissue culture in vitro technology. Nowadays, it is well known that the cultivation through the Temporary Immersion System (TIS) in a bioreactor has considerable advantages both for the in vitro mass production of the plants and for the production of secondary metabolites. The present review focuses on the application of TIS during the last two decades to produce biomass and bioactive compounds from medicinal plants. Indeed, almost one hundred papers are discussed, and they particularly focus on the effects of the culture system, vessel design and equipment, immersion time and frequency, and substrate composition for 88 medicinal species in TIS bioreactor culture.

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Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Cited by 13 publications

References 38 publications

Use of Liquid Culture with the ElecTIS Bioreactor for Faster Recovery of Blackberry (Rubus fruticosus L.) Shoots from Conservation at 4 °C

Use of Liquid Culture with the ElecTIS Bioreactor for Faster Recovery of Blackberry (Rubus fruticosus L.) Shoots from Conservation at 4 °C

Micropropagation and Acclimatization of Monstera deliciosa Liebm. ‘Thai Constellation’

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

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