Improving of hydrolases biosythesis by solid-state fermentation of Penicillium camemberti on rapeseed cake

Boratyński, Filip; Szczepańska, Ewa; Grudniewska, Aleksandra; Gniłka, Radosław; Olejniczak, Teresa

doi:10.1038/s41598-018-28412-y

Cited by 30 publications

(22 citation statements)

References 55 publications

(69 reference statements)

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“…This had also a positive effect on the dry weight of the mycelial biomass obtained on all three media (Table 2). Some studies have described a positive effect of Ca 2+ ions increasing the biosynthesis of lipases, e.g., lipases from Bacillus cereus [44] and Penicillium camemberti [45]. However, in this study, the increase in lipase biosynthesis by C. pannorum A-1 does not seem to be caused directly by Ca 2+ ions, because there were no significant differences in the activities between the BMC and BM media (without olive oil and glucose).…”

Section: Discussioncontrasting

confidence: 74%

Biomass of a Psychrophilic Fungus as a Biocatalyst for Efficient Direct Esterification of Citronellol

et al. 2021

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A biomass-bound lipase from psychrophilic Chrysosporium pannorum A-1 is an efficient biocatalyst for direct esterification of β-citronellol and acetic acid in an organic solvent. The biomass is effectively produced by fungal submerged culture at 20 ℃, which results in lower energy consumption during the production of biocatalyst. Supplementation of the culture medium with calcium carbonate together with olive oil contributed to a significant increase in the active biomass of mycelium in one batch culture and increased the efficiency of the biocatalyst. Biomass-bound lipase showed high catalytic activity in a broad temperature range of 30–60 °C and stability up to 70 °C. A maximum molar conversion value of 98% was obtained at 30 °C in n-hexane using a 2:1 alcohol-to-acid molar ratio and 3% w/v of the biocatalyst within 24 h. The high equimolar concentration of the substrates (200 mM) did not have an adverse effect on mycelial biomass activity. Dry mycelium of C. pannorum is a promising biocatalyst for large-scale biosynthesis of citronellyl acetate, given its low-cost production, high activity at low temperatures, and reusability in a minimum of seven 24-h biocatalytic cycles.

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Section: Discussioncontrasting

confidence: 74%

Biomass of a Psychrophilic Fungus as a Biocatalyst for Efficient Direct Esterification of Citronellol

et al. 2021

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“…This further proved that B. amyloliquefaciens CX-20 could produce many enzymes to hydrolyze the carbohydrates of RSC into reducing sugars. It has been reported that rapeseed oil could be used as a source of carbon to ferment microbial products such as lipase [23], erythromycin [30] and isocitric acid [31]. However, whether the residual oil in RSC could be used as a carbon source for iturin A production by B. amyloliquefaciens CX-20 was still unclear.…”

Section: Resultsmentioning

confidence: 99%

“…However, unlike carbohydrases and proteases [16], lipases have not been widely used for the pretreatment of rapeseed by-products to improve their value for microbial production. It has been reported that RSC could be used as a valuable raw material for producing lipases and proteases due to its high content of lipids and proteins [23]. Therefore, it seems feasible to use lipases to solve the problem of high oil content in RSC.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20

Chen

et al. 2019

BMC Biotechnol

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BackgroundRapeseed cake (RSC), as the intermediate by-product of oil extraction from the seeds of Brassica napus, can be converted into rapeseed meal (RSM) by solvent extraction to remove oil. However, compared with RSM, RSC has been rarely used as a raw material for microbial fermentation, although both RSC and RSM are mainly composed of proteins, carbohydrates and minerals. In this study, we investigated the feasibility of using untreated low-cost RSC as nitrogen source to produce the valuable cyclic lipopeptide antibiotic iturin A using Bacillus amyloliquefaciens CX-20 in submerged fermentation. Especially, the effect of oil in RSC on iturin A production and the possibility of using lipases to improve the iturin A production were analyzed in batch fermentation.ResultsThe maximum production of iturin A was 0.82 g/L at the optimal initial RSC and glucose concentrations of 90 and 60 g/L, respectively. When RSC was substituted with RSM as nitrogen source based on equal protein content, the final concentration of iturin A was improved to 0.95 g/L. The production of iturin A was further increased by the addition of different lipase concentrations from 0.1 to 5 U/mL into the RSC medium for simultaneous hydrolysis and fermentation. At the optimal lipase concentration of 0.5 U/mL, the maximal production of iturin A reached 1.14 g/L, which was 38.15% higher than that without any lipase supplement. Although rapeseed oil and lipase were firstly shown to have negative effects on iturin A production, and the effect would be greater if the concentration of either was increased, their respective negative effects were reduced when used together.ConclusionsAppropriate relative concentrations of lipase and rapeseed oil were demonstrated to support optimal iturin A production. And simultaneous hydrolysis with lipase and fermentation was an effective way to produce iturin A from RSC using B. amyloliquefaciens CX-20.

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“…RSM is a good protein source for the microbial fermentation of high‐value‐added products (Wang et al ., 2010; Kiran et al ., 2013; Chatzifragkou et al ., 2014; Jaszek et al ., 2016; Prendecka et al ., 2016; Boratyński et al ., 2018), but its particular physical and chemical structure limited the nutrients to be directly assimilated by the majority of industrial microorganisms without pretreatment (Wang et al ., 2010). Consequently, the application of RSM hydrolysate produced following fungal pretreatment as a feedstock for microbial bioconversions has been intensively investigated (Wang et al ., 2010; Kiran et al, 2012, 2013; García et al ., 2013; Chatzifragkou et al ., 2014; Salakkam et al ., 2017; Salakkam and Webb, 2018).…”

Section: Discussionmentioning

confidence: 99%

The unconventional adverse effects of fungal pretreatment on iturin A fermentation by Bacillus amyloliquefaciens CX‐20

Chen

Wang

Gong

et al. 2020

Microbial Biotechnology

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Fungal pretreatment is the most common strategy for improving the conversion of rapeseed meal (RSM) into value-added microbial products. It was demonstrated that Bacillus amyloliquefaciens CX-20 could directly use RSM as the sole source of all nutrients except the carbon source for iturin A fermentation with high productivity. However, whether fungal pretreatment has an impact on iturin A production is still unknown. In this study, the effects of fungal pretreatment and direct bio-utilization of RSM for iturin A fermentation were comparatively analysed through screening suitable fungal species, and evaluating the relationships between iturin A production and the composition of solid fermented RSM and liquid hydrolysates. Three main unconventional adverse effects were identified. (1) Solid-state fermentation by fungi resulted in a decrease of the total nitrogen for B. amyloliquefaciens CX-20 growth and metabolism, which caused nitrogen waste from RSM. (2) The released free ammonium nitrogen in liquid hydrolysates by fungal pretreatment led to the reduction of iturin A. (3) The insoluble precipitates of hydrolysates, which were mostly ignored and wasted in previous studies, were found to have beneficial effects on producing iturin A. In conclusion, our study verifies the unconventional adverse effects of fungal pretreatment on iturin A production by B. amyloliquefaciens CX-20 compared with direct bioutilization of RSM.

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Improving of hydrolases biosythesis by solid-state fermentation of Penicillium camemberti on rapeseed cake

Cited by 30 publications

References 55 publications

Biomass of a Psychrophilic Fungus as a Biocatalyst for Efficient Direct Esterification of Citronellol

Biomass of a Psychrophilic Fungus as a Biocatalyst for Efficient Direct Esterification of Citronellol

Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20

The unconventional adverse effects of fungal pretreatment on iturin A fermentation by Bacillus amyloliquefaciens CX‐20

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