Improving glycemic control in model mice with type 2 diabetes by increasing superoxide dismutase (SOD) activity using silk fibroin hydrolysate (SFH)

Jung, Harry; Kim, Yoo Yeon; Kim, Boyoung; Nam, Hajin; Suh, Jun-Gyo

doi:10.1016/j.bbrc.2017.09.066

Cited by 21 publications

(14 citation statements)

References 28 publications

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“…In regard to the literature dealing with this topic, Harnedy et al [81] and Parthsarathy et al [98] reported a protein hydrolysate from blue whiting and boarfish with in vitro and in vivo antidiabetic properties, using cell cultures and mice. Similar research was carried out by Jung et al [232] with silk fibroin hydrolysate, and by Hsieh et al [162] with milk proteins. Mochida et al [233] reported that zein-derived peptides induced glycemic regulation via GLP-1 secretion, and DPP-IV inhibition in rats, whereas Ishikawa et al [234] obtained similar results by employing rice-derived peptides.…”

Section: Vertebrates Modelssupporting

confidence: 71%

Antidiabetic Food-Derived Peptides for Functional Feeding: Production, Functionality and In Vivo Evidences

Rivero‐Pino

Espejo-Carpio

Guadix

2020

Foods

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Bioactive peptides released from the enzymatic hydrolysis of food proteins are currently a trending topic in the scientific community. Their potential as antidiabetic agents, by regulating the glycemic index, and thus to be employed in food formulation, is one of the most important functions of these peptides. In this review, we aimed to summarize the whole process that must be considered when talking about including these molecules as a bioactive ingredient. In this regard, at first, the production, purification and identification of bioactive peptides is summed up. The detailed metabolic pathways described included carbohydrate hydrolases (glucosidase and amylase) and dipeptidyl-peptidase IV inhibition, due to their importance in the food-derived peptides research field. Then, their characterization, concerning bioavailability in vitro and in situ, stability and functionality in food matrices, and ultimately, the in vivo evidence (from invertebrate animals to humans), was described. The future applicability that these molecules have due to their biological potential as functional ingredients makes them an important field of research, which could help the world population avoid suffering from several diseases, such as diabetes.

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Section: Vertebrates Modelssupporting

confidence: 71%

Antidiabetic Food-Derived Peptides for Functional Feeding: Production, Functionality and In Vivo Evidences

Rivero‐Pino

Espejo-Carpio

Guadix

2020

Foods

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“…Flavonoids contained in torbangun leaves extract were able to provide protection against oxidative stress, which can be partially associated with a mechanism involving SOD activity. Jung et al (2017) stated that this increase in SOD activity was able to prevent the death of pancreatic β-cells, thereby being able to recover hyperglycemia and insulin intolerance.…”

Section: Animal Testmentioning

confidence: 99%

“…It has been shown in previous study that the pancreatic β-cells in DM subjects had a very low SOD (Jung et al 2017). Thus, through this mechanism, the flavonoids in torbangun leaves extract can act as an antioxidant that help the DM recovery process (Suryowati et al 2015b).…”

Section: Introductionmentioning

confidence: 95%

Effects of Torbangun Leaves(Coleus amboinicus Lour) Extract on Blood Glucose and Super Oxide Dismutase Activity in Hyperglycemic Rats

et al. 2019

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This research aimed to analyze the yield of torbangun extract, flavonoid content, and antioxidant activity of TE-S and its effect on the activity of SOD enzymes and blood glucose level in hyperglycemic rats as a DM animal model. The water content of simplicia was measured by the gravimetric method. The antioxidant activity of TE-S was determined using the DPPH method, while the total flavonoid was measured using spectrophotometry. The study was a completely randomized design using 25 Sprague Dawley rats. Rats were divided into four groups, namely NG (negative control, hyperglycemic rats), N (normal rats), HIM (control of metformin drugs 62.5 mg/kg of BW), and HIT (TE-S 620 mg/kg of BW). The treatment was carried out for 14 days. FBG levels were taken on day 0, 4, 7, 11, and 14, measured using a glucometer, while blood serum SOD levels were measured using ELISA. The study showed the water content of torbangun simplicia was 7.99% and TE-S yield from simplicia was 4.69%. TE-S contains total flavonoids of 3.91% and antioxidant activity (IC 50) of 306.28 ppm with a standard of 1 ppm vitamin C. TE-S treatment significantly decreased FBG (p=0.005, α=0.01) and increased SOD levels in hyperglycemic rats. TE-S has the potential to increase blood serum SOD levels by contributing to the availability of antioxidants and decreasing blood glucose levels in hyperglycemic rats.

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“…Silk fibroins can be hydrolyzed by enzymes or acids, and silk fibroins hydrolyzed with a mixture of proteinases were reported to have anti-hyperglycemic activity in diabetic experimental animals [17]. However, the process is expensive and complicated, and thus, we considered that acid hydrolyzed silk fibroins might have similar anti-diabetic activities.…”

Section: Discussionmentioning

confidence: 99%

Acid Hydrolyzed Silk Peptide Consumption Improves Anti-Diabetic Symptoms by Potentiating Insulin Secretion and Preventing Gut Microbiome Dysbiosis in Non-Obese Type 2 Diabetic Animals

et al. 2020

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Silk fibroin hydrolysates have been reported to reduce hyperglycemia, but the mechanism has not been determined in Asian type 2 diabetes (T2DM). We hypothesized that the consumption of acid hydrolyzed silk peptides (SPs) alleviates hyperglycemia by improving insulin sensitivity and subsequently normalizing glucose-stimulated insulin secretion in T2DM. We investigated this hypothesis in a partial pancreatectomized (Px) rat model. Px rats was assigned randomly to the following six groups and fed assigned diet for 8 weeks: the Px-control (0.5 g/kg/day dextrin), the SP-L (0.05 g/kg/day), the SP-M (0.1 g/kg/day), the SP-H (0.5 g/kg/day), the positive-control (40 mg/kg/day metformin), or the normal-control (sham-operated rats; 0.5 g/kg/day dextrin). SPs contained high levels of glycine, alanine, and serine. We found SPs dose-dependently increased food efficiency and body weight gain in Px rats. Animals in the Px-control group rats exhibited lower glucose metabolism, as evidenced by impaired glucose-stimulated insulin secretion coupled with impaired insulin sensitivity, and reduced bone mineral density (BMD) and lean body mass (LBM), compared to the normal-control. SPs and metformin similarly partially protected against Px-induced BMD loss in the lumbar spine and femur. Px-induced decreases in LBM were dose-dependently prevented by SPs, and muscle forces in the SP-M and SP-H groups were maintained at the normal-control level. Glucose tolerance was dose-dependently improved by SPs as determined by oral glucose tolerance and oral maltose tolerance tests, and glucose tolerances were similar in the SP-H and positive-control groups. Insulin tolerance, an index of insulin sensitivity, was dose-dependently enhanced by SPs, and the SP-H group exhibited better insulin tolerance than the positive-control group as determined by intraperitoneal insulin sensitivity testing. Insulin secretory capacity assessed using a hyperglycemic clamp improved in the following order: Px-control <SA-L <SA-M <positive-control <SA-H <normal-control. SP-M prevented gut microbiota dysbiosis. In conclusion, SPs administered at 0.1–0.5 g/kg/day improved glucose regulation by potentiating both insulin secretion and insulin sensitivity in non-obese T2DM rats.

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Improving glycemic control in model mice with type 2 diabetes by increasing superoxide dismutase (SOD) activity using silk fibroin hydrolysate (SFH)

Cited by 21 publications

References 28 publications

Antidiabetic Food-Derived Peptides for Functional Feeding: Production, Functionality and In Vivo Evidences

Antidiabetic Food-Derived Peptides for Functional Feeding: Production, Functionality and In Vivo Evidences

Effects of Torbangun Leaves(Coleus amboinicus Lour) Extract on Blood Glucose and Super Oxide Dismutase Activity in Hyperglycemic Rats

Acid Hydrolyzed Silk Peptide Consumption Improves Anti-Diabetic Symptoms by Potentiating Insulin Secretion and Preventing Gut Microbiome Dysbiosis in Non-Obese Type 2 Diabetic Animals

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