Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail

Bussamra, Bianca Consorti; Freitas, Sindélia; Costa, Aline Carvalho da

doi:10.1016/j.biortech.2015.03.117

Cited by 59 publications

(34 citation statements)

References 28 publications

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“…However, the high cost of producing these biocatalysts remains a problem for the conversion of lignocellulosic material via enzymatic hydrolysis and is a limitation to the production of ethanol from vegetable biomass (Bussamra et al, 2015). In this sense, recent studies have sought to reduce the final cost of these enzymes by prospecting strains with a potential to produce high concentrations of enzymes by low-cost methods (Costa et al, 2016;Delabona et al, 2013;Lan et al, 2013;Santos et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Catalytic properties of cellulases and hemicellulases produced by Lichtheimia ramosa: Potential for sugarcane bagasse saccharification

Garcia

Santos

Bocchini

et al. 2018

Industrial Crops and Products

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A B S T R A C TThe use of microbial enzyme cocktails for the conversion of polysaccharides of plant origin into fermentable sugars is a global trend. The objectives of this study were to optimize the production of cellulases and hemicellulases by the fungus Lichtheimia ramosa, evaluate the catalytic properties of the produced enzymes, and apply these biocatalysts in the saccharification of sugarcane bagasse. The production of carboxymethylcellulase (CMCase), β-glucosidase, xylanase, and β-xylosidase by L. ramosa were 168.1 ± 2.2, 270.4 ± 8.9, 34 ± 0.8, and 199.2 ± 2.6 U/g of dry substrate, respectively. The optimum pH for the activity of the enzymes ranged between 4.5 and 5.5, and the optimum temperature varied between 55°C and 65°C. The enzymes were stable within a wide range of pH, and approximately 95% of their original activity was preserved when incubated for 1 h at 55°C. The half-lives (t 1/2 ) of CMCase, β-glucosidase, xylanase, and β-xylosidase were 68, 59, 52, and 54 min, respectively, when incubated at 60°C. The enzymes were stable in solutions containing ethanol (10%), and the kinetic parameters (K m and V max ) demonstrated that β-glucosidase from L. ramosa was competitively inhibited by glucose. The enzyme extract produced by L. ramosa was used for the saccharification of sugarcane bagasse pretreated with glycerol and the highest yield of glucose (10.66%) was obtained at 24 h of hydrolysis. The characteristics of the enzymes, combined with the efficiency in hydrolyzing sugarcane bagasse, allow for the application of this enzyme extract to processes of cellulose saccharification for the production of second-generation ethanol.

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Section: Introductionmentioning

confidence: 99%

Catalytic properties of cellulases and hemicellulases produced by Lichtheimia ramosa: Potential for sugarcane bagasse saccharification

Garcia

Santos

Bocchini

et al. 2018

Industrial Crops and Products

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“…The glucose releasing efficiency of the optimized enzyme system in this study is also considerably higher than the T. reesei enzyme cocktail (0.021 g glc /FPU) comprising T. reesei cellobiohydrolase enriched fraction, endoglucanase, and b-glucosidase previously reported by Bussamra et al (2015). However, it should be noted that these observations cannot be directly compared owing to variation in the substrates, enzyme combination, and reaction conditions.…”

Section: Cooperative Enzyme Interaction On Pretreated Rice Strawmentioning

confidence: 48%

Synergistic action of recombinant accessory hemicellulolytic and pectinolytic enzymes to Trichoderma reesei cellulase on rice straw degradation

Laothanachareon

Bunterngsook

Suwannarangsee

et al. 2015

Bioresource Technology

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“…Researches concerning optimization of enzymatic cocktails for different conditions through statistical methods have been reported in the literature. Some of these studies comprehend the use of mixtures of purified enzymes for lignocellulosic biomass hydrolysis and have achieved different maximum conversions to glucose, such as Banerjee et al (yield of 40%), Gao et al (yield of 80%), Billard et al (yield of 64%), and Bussamra et al (yield of 72%). These differences between hydrolysis efficiencies are related to the various operational conditions employed in each work, like feedstock composition, solid and protein loadings, and others.…”

Section: Resultsmentioning

confidence: 99%

Design of an enzyme cocktail consisting of different fungal platforms for efficient hydrolysis of sugarcane bagasse: Optimization and synergism studies

Arias

Modesto

Polikarpov

et al. 2016

Biotechnology Progress

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Lignocellulosic materials represent a very important and promising source of renewable biomass. In order to turn them into fermentable sugars, synergism among the different enzymes that carry out bioconversion of these materials is one of the main factors that should be considered. Experimental mixture design was performed to optimize the proportion of enzymes produced by native strains of Trichoderma harzianum IOC 3844, Penicillium funiculosum ATCC 11797, and Aspergillus niger ATCC 1004, resulting in a proportion of 15, 50, and 35%, respectively. This mixture was able to hydrolyze 25 g/L of pretreated sugarcane bagasse with 91% of yield after 48 h of enzymatic reaction. Synergism along the hydrolysis process, besides the influence of lignin, hemicellulose, and solids loading, were also studied. Response surface methodology (RSM) based on Central Composite Rotatable Design was used to optimize solids and protein loadings to increase glucose release and enzymatic hydrolysis yield. The optimum solid and protein loadings established with RSM were 196 g/L and 24 mg/g cellulose, respectively, and under these conditions (94.1 ± 8) g/L of glucose were obtained, corresponding to a hydrolysis yield of 64%. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1222-1229, 2016.

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Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail

Cited by 59 publications

References 28 publications

Catalytic properties of cellulases and hemicellulases produced by Lichtheimia ramosa: Potential for sugarcane bagasse saccharification

Catalytic properties of cellulases and hemicellulases produced by Lichtheimia ramosa: Potential for sugarcane bagasse saccharification

Synergistic action of recombinant accessory hemicellulolytic and pectinolytic enzymes to Trichoderma reesei cellulase on rice straw degradation

Design of an enzyme cocktail consisting of different fungal platforms for efficient hydrolysis of sugarcane bagasse: Optimization and synergism studies

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