Synergistic action of recombinant accessory hemicellulolytic and pectinolytic enzymes to Trichoderma reesei cellulase on rice straw degradation

Laothanachareon, Thanaporn; Bunterngsook, Benjarat; Suwannarangsee, Surisa; Eurwilaichitr, Lily; Champreda, Verawat

doi:10.1016/j.biortech.2015.09.053

Cited by 57 publications

(19 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These findings demonstrate the potential of formulating active synergistic enzyme systems from various microbial sources in order to maximize the hydrolysis efficiency on specific lignocellulosic substrates. The glucose releasing efficiency of the synergistic ternary enzyme system developed in this study was higher than that reported for Accellerase®1500 (81 mg glc/FPU) and the synergistic enzyme systems based on that enzyme, which was in the range of 122–173 mg/FPU [33]. The efficiency of the ternary enzyme mixture is in the same range to that reported for a synergistic system comprising T. reesei cellulase, a crude enzyme from A. aculeatus and a bacterial expansin [19] (229 mg glc/FPU) and between T. reesei cellulase and enzyme from A. awamori [42] (200 mg glc/FPU).…”

Section: Resultsmentioning

confidence: 76%

“…Glycosyl hydrolases from the metagenome of microflora present in sugarcane bagasse can enhance reducing sugar yield of T. reesei cellulase [41]. A recent work also showed strong synergistic action of a commercial cellulase, Acellerase®1500 with an endo-xylanase, pectate lyase, and a hemicellulose side chain cleaving α-arabinofuranosidase [33]. These findings demonstrate the potential of formulating active synergistic enzyme systems from various microbial sources in order to maximize the hydrolysis efficiency on specific lignocellulosic substrates.…”

Section: Resultsmentioning

confidence: 88%

See 1 more Smart Citation

Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification

et al. 2016

Self Cite

View full text Add to dashboard Cite

BackgroundEfficient hydrolysis of lignocellulosic materials to sugars for conversion to biofuels and chemicals is a key step in biorefinery. Designing an active saccharifying enzyme system with synergy among their components is considered a promising approach.ResultsIn this study, a lignocellulose-degrading enzyme system of Chaetomium globosum BCC5776 (CG-Cel) was characterized for its activity and proteomic profiles, and synergism with accessory enzymes. The highest cellulase productivity of 0.40 FPU/mL was found for CG-Cel under the optimized submerged fermentation conditions on 1% (w/v) EPFB (empty palm fruit bunch), 2% microcrystalline cellulose (Avicel®) and 1% soybean meal (SBM) at 30 °C, pH 5.8 for 6 d. CG-Cel worked optimally at 50–60 °C in an acidic pH range. Proteomics analysis by LC/MS/MS revealed a complex enzyme system composed of core cellulases and accessory hydrolytic/non-hydrolytic enzymes attacking plant biopolymers. A synergistic enzyme system comprising the CG-Cel, a β-glucosidase (Novozyme® 188) and a hemicellulase Accellerase® XY was optimized on saccharification of alkaline-pretreated rice straw by a mixture design approach. Applying a full cubic model, the optimal ratio of ternary enzyme mixture containing CG-Cel: Novozyme® 188: Accellerase® XY of 44.4:20.6:35.0 showed synergistic enhancement on reducing sugar yield with a glucose releasing efficiency of 256.4 mg/FPU, equivalent to a 2.9 times compared with that from CG-Cel alone.ConclusionsThe work showed an approach for developing an active synergistic enzyme system based on the newly characterized C. globosum for lignocellulose saccharification and modification in bio-industries.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-016-0312-7) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 88%

Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lignin, as a polyphenolic compound, was degraded in a variety of ways with UV-A irradiation, such as the breaking of chemical bonds between lignin monomers and the rupture of benzene rings (Brandt et al 2010;Smith et al 2010). Cellulose and hemicellulose, surrounded by lignin, were then exposed to UV radiation and plentiful cellulolytic and hemicellulolytic enzymes as the lignin degradation progressed (Brink et al 2014;Laothanachareon et al 2015). As a result of the partial degradation of lignocellulose, the concentration of DOC increased from days 50 to 110.…”

Section: Straw Chemical Composition Analysismentioning

confidence: 99%

Straw Degradation Behaviors under Different Conditions of Relative Air Humidity and Ultraviolet-A Irradiation

Huang

et al. 2016

BioResources

View full text Add to dashboard Cite

In this study, straw was degraded continuously for 150 days under one of three levels of relative air humidity (RH) (90%, 60%, or 30%) to estimate the effect of humidity on straw biodegradation. Moreover, straw was treated with ultraviolet (UV)-A irradiation + 90% RH for 180 days to evaluate the interaction between photodegradation and biodegradation. The effects of 30% and 60% RH on straw degradation was inconspicuous. Straw mass losses at 90% RH and UV-A + 90% RH were 18.5% and 39.1%, respectively. BIOLOG analysis showed that filamentous fungi played a major role in straw biodegradation. Thermogravimetric analysis showed that treatment with UV-A + 90% RH tended to increase the maximum pyrolysis rate and decreased the initial pyrolysis temperature. Compared with 90% RH, infrared spectra analysis showed that functional groups of UV-A + 90% RH treatment, e.g., -CH, -C=O, and the benzene ring structure, clearly decreased. Strawdegrading bacteria were observed by scanning electron microscopy at the beginning and end of UV-A + 90% RH treatment. Results highlight the role of humidity in the degree of straw biodegradation by filamentous fungi. Straw degradation is accelerated by the combined action of photodegradation and biodegradation under high UV-A irradiation and high humidity.

show abstract

“…Several tailor-made enzyme mixtures from various microbial origins have been reported for hydrolysis of pretreated wheat straw, rice straw and corn stover. 12,13 Most works have tested different combinations of crude or commercial enzymes however, with very few reports on synergistic mixtures of recombinant or isolated hydrolases from different families with dened specicities, for examples, on hydrolysis of wheat straw and rice straw. [13][14][15] Further enhancements of the released sugar yield with additional non-hydrolytic enzymes or auxiliary components (e.g.…”

Section: -7mentioning

confidence: 99%

“…Recombinant plasmid pPICZa-Xyn encoding a GH10 endo-b-1,4-xylanase from A. aculeatus BCC17849 was constructed and reported in Laothanachareon et al, 2015, 13 and the recombinant xylanase (XYN) was prepared as described below. The commercial raw starch degrading enzyme STARGEN™ 002, containing a-amylase from Aspergillus kawachii and gluco-amylase from Trichoderma reseei was purchased from Dupont (Rochester, NY).…”

mentioning

confidence: 99%

Optimization of a minimal synergistic enzyme system for hydrolysis of raw cassava pulp

et al. 2017

Self Cite

View full text Add to dashboard Cite

Cassava pulp is an underused agricultural by-product comprising residual starch granules entrapped in cell wall polysaccharides, making it unique from other lignocellulosic wastes in terms of enzymatic processing.In this study, a synergistic system comprising a minimal set of enzymes tailor-made for efficient bioprocessing of raw cassava pulp was developed. The fiber-degrading enzyme mixture comprises an endoglucanase (Cel12), b-glucosidase (BGL), endo-b-1,4-xylanase (XYN) and endo-polygalacturonase (EPG)from Aspergillus aculeatus and a cellobiohydrolase I (Cel7A) from Talaromyces cellulolyticus was initially optimized using the experimental design approach. A glucose recovery yield of 91.3% based on the total starch and cellulose content was obtained from saccharification of cassava pulp using the combination of

show abstract

Synergistic action of recombinant accessory hemicellulolytic and pectinolytic enzymes to Trichoderma reesei cellulase on rice straw degradation

Cited by 57 publications

References 34 publications

Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification

Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification

Straw Degradation Behaviors under Different Conditions of Relative Air Humidity and Ultraviolet-A Irradiation

Optimization of a minimal synergistic enzyme system for hydrolysis of raw cassava pulp

Contact Info

Product

Resources

About