Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS)

Abstract

Seeds of a Chinese traditional medicine plant, Cochinchina momordica were used in the present study for the improvement of influenza vaccine (H5N1) in chicken. Crude extraction from Cochinchina momordica seed (ECMS) was obtained by ethanol extraction method. In experiment No. 1, two weeks old chickens were immunized with influenza vaccine (H5N1) alone or combined with ECMS (5, 10, 20, 40 and 80 microg/dose). Serum IgG antibody levels (by ELISA) as well as effects on daily weight gain were measured on 0, 7, 14 and 28th day after immunization. Results revealed that all ECMS groups numerically increased the antibody levels while 10 and 20 microg/dose groups significantly (P<0.05) enhanced total IgG antibody on day 28, when compared with control. Average daily weight gain was also significantly higher in 20 microg/dose ECMS group. Adjuvant effect was also confirmed in experiment No. 2 when chickens were immunized with 20 microg/dose ECMS and antibody titer was measured through hemagglutination inhibition (HI). It is concluded that ECMS has potential to improve the immune responses and deserve further study as an adjuvant.

Momordica cochinchinensis (L.) Spreng: Aril Extract Prevents Adverse Reproductive Parameters of Male Rats Induced with Valproic Acid

“…In further studies, the biochemical and molecular analyses will be performed to explain additional mechanisms of how the MC could prevent such adverse effects. The protective tissue damage effect demonstrated in this study is a phytotherapeutic property of MC extract besides other pharmacological properties shown in previous reports (Zheng et al;Jung et al, 2013aJung et al, , 2013bLiu et al;Chuethong et al;Tien et al;Kang et al;Tsoi et al;Xiao et al, 2007Xiao et al, , 2009Rajput et al, 2007Rajput et al, , 2010. Moreover, MC extract indeed did not affect any male reproductive parameters including testicular histology as compared to the control.…”

Enhancement of Serological Immune Responses to Foot-and-Mouth Disease Vaccine by a Supplement Made of Extract of Cochinchina Momordica Seeds

“…In addition, ECMS has been found effective in stimulation of the immune response to vaccination against H5N1 avian influenza in chickens (24). In this study, we demonstrated that ECMS and oil emulsion synergistically promoted the production of IgG1 and IgG2 elicited by FMDV antigens in a guinea pig model, as shown in Fig.…”

“…(ECMS), has an adjuvant effect on the immune responses elicited by ovalbumin (OVA), with a much lower hemolytic activity (30). ECMS has also been proven effective in enhancing immune responses to vaccination against avian influenza (H5N1) in chickens (24). The medical use of the seeds has been described in ancient Chinese medical literature, Kai Bao Materia Medica, from the Song Dynasty (AD 793) (10) and is currently included in both Chinese pharmacopoeia (5) and Chinese veterinary pharmacopoeia (6).…”

Transient expression of chicken alpha interferon gene in lettuce

“…Many viruses are threatening the health and welfare of poultry and have even struck the poultry industry and brought tremendous economic losses to the world, such as the case of the highly pathogenic avian influenza (HPAI) in recent years. At present, vaccina- tion is the great asset for outbreak containment of avian disease (Habib et al, 2006;Rajput et al, 2007). However, the intensive use of vaccines is driving virus to increase its virulence such as the case of the MDV with greater virulence in vaccinated chickens (Witter, 1997).…”