Transient expression of chicken alpha interferon gene in lettuce

Abstract

Abstract:We investigated the possibility of producing chicken alpha interferon (ChIFN-α) in transgenic plants. The cDNA encoding ChIFN-α was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system. The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF). The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.

Khả năng ức chế virus gây bệnh Gumboro trên gà 3 tuần tuổi của Interferon Alpha gà

“…Ngoài ra ChIFN-α được chú ý vì nó là hợp chất: có phổ hoạt động rộng (tác động lên nhiều loại virus khác nhau trên gia cầm), gần giống với các hợp chất tự nhiên, hiệu quả cao, tiện lợi khi sử dụng. Các gene mã hóa interferon alpha gà tái tổ hợp (rChIFN-α) đã lần lượt được tạo dòng, biểu hiện, và đều cho hoạt tính sinh học khi thử nghiệm in vitro và cả in vivo (Hou et al, 2011;Li, De-gang, Yong-jun, & Yi, 2008;Meng et al, 2011). Tuy nhiên, để sản xuất rChIFN-α với số lượng lớn và giá thành thấp phù hợp cho chăn nuôi thì phải cân nhắc các hệ thống biểu hiện.…”

Insight into Alternative Approaches for Control of Avian Influenza in Poultry, with Emphasis on Highly Pathogenic H5N1

“…An innovative application of ChIFN-α to antagonize AIV infection in poultry through direct oral feeding or drinking water has received more attention than other components [164,165,166,167,168]. Sekellick et al [169] showed that up to 60% of investigated AIV population belonged to the HPAI H5N9 subtype were highly sensitive to the inhibitory effects of ChIFN-α.…”

“…Nevertheless, the cost of mass production of chicken cytokines is still too high to be applied in large-scale in poultry industry [165]. Moreover, protein stability, host-specificity and labor associated with mass administration of chicken cytokines under field conditions require significant improvement [172].…”

Recombinant Cytokines from Plants

“…The first reports about the plan-based production of human interferon date back to the beginning of the 90 s [ 56 ], however it was the usage of transplanstomic plants that allowed for the huge increase of yield, up to 20% of TSP [ 45 ]. It is worth mentioning that not only human interferons but also interferons from chicken [ 49 ] and salmon [ 50 ] were successfully produced in plant-based systems.…”