Improvement of the Aluminum Borate Whisker-Mediated Method of DNA Delivery into Rice Callus

Mizuno, Kiyotaka; Takahashi, Wataru; Ohyama, Takashi; Shimada, Takehiko; Tanaka, Osamu

doi:10.1626/pps.7.45

Cited by 19 publications

(14 citation statements)

References 17 publications

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“…DNA delivery in the whisker method is increased by increasing the duration of agitation, agitation speed and amount of whiskers. However cell damage occurs when agitation is severe, extra prolonged and too large an amount of whiskers resulting in severe damage losing cell viability (Petolino et al, 2000;Mizuno et al, 2004). GUS expression units were maximum when mixing speed was 8 for 30 min using 20mg of ABW where as most of silicon carbide whisker preparations were made at the rate of 5% (Asad et al, 2008).…”

Section: Mixing Methodsmentioning

confidence: 99%

Silicon Carbide Whisker-mediated Plant Transformation

Asad¹,

Arshad²

2011

Properties and Applications of Silicon Carbide

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With the advancement in molecular biology, several metabolic and physiological processes have been elucidated at molecular levels discovering the involvement of different genes. Since the advent of plant transformation 33 years ago, use of plant transformation techniques sparked an interest in fundamental and applied research leading to the development of biological and physical methods of foreign DNA delivery into 130 plant species. Modern molecular biology tools have developed rich gene sources which are waiting to be transformed into plant species. But unavailability of efficient transformation methods is a major hurdle to expedite the delivery of these genes into plants. Ever-expanding available gene pools in the era of third generation transgenic plants stressing the delivery of multiple genes for different traits; development and application of new transformation methods is the big need of the time to meet the future challenges for plant improvement. In recent years, silicon-carbide whiskers have proven valuable and effective alternative in which silicon carbide fibers are mixed with plant cells and plasmid DNA, followed by vortexing/oscillation. Cell penetration appears to occur thus whiskers function as numerous fine needles, facilitating DNA entry into cells during the mixing process. This technique is simple, easy and an inexpensive transformation method to deliver the DNA into monocot and dicot plant species. Whiskers, cells and plasmid DNA are combined in a small tube and mixed on a vortex or oscillating mixer. In this chapter we will discuss the use of silicon carbide fibers/whiskers to transform and produce different transgenic plants. This chapter will help the reader to know about emerging applications of silicon carbide and other fibers in the delivery of foreign DNA into plants, and critical parameters affecting DNA delivery efficiency will also be discussed. IntroductionPlant Cell wall is commonly found as the non-living barrier in the ways of DNA deliver technologies being attempted for plant genetic engineering. In case of biological systems, the cell wall is dissolved by cell wall degrading enzymes secreted by donor host for contact of donor cell with recipient cell allowing exchange of biological materials along with net DNA delivery into recipient cells. But this limitation cannot be overcome in monocots which are 15 www.intechopen.com

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Section: Mixing Methodsmentioning

confidence: 99%

Silicon Carbide Whisker-mediated Plant Transformation

Asad¹,

Arshad²

2011

Properties and Applications of Silicon Carbide

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“…A higher whisker quantity and prolonged shaking can increase the transformation efficiency, whereas cell survival and regeneration are adversely affected. In other words, the whisker quantity, mixing speed and survival rate have to be well-balanced to optimize the efficiency (Frame et al 1994, Petolino et al 2000, Mizuno et al 2004, Keshavareddy et al 2013. Moreover, callus age affected the transformation efficiency greatly in different crops.…”

Section: Rt-pcr Analysismentioning

confidence: 99%

Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease

Hassan

Akram

Ali

et al. 2016

Crop Breed. Appl. Biotechnol.

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“…Therefore, there has been a search for other possible alternative, which could be used for similar DNA transfer purposes, as a consequence; the use of aluminum borate whiskers (ABW) was appeared [41]. The same group researchers improved this method by using multidirectional shaker instead of ordinary vortex.…”

Section: Silicon Carbide-mediated Transformationmentioning

confidence: 99%

Novel Techniques for Gene Delivery into Plants and Its Applications for Disease Resistance in Crops

Rashid¹,

Lateef²

2016

AJPS

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From the early past to the present, biotechnologies have produced the ability to genetically transform a wide variety of plant species. The plant transformation technologies have changed the face of agriculture and plant biology. Plant genetic transformation is one of the key technologies for crop improvement in addition to emerging approach for producing recombinant proteins in plants. Both plastid genomes and plant nuclear can be genetically modified. Until now, essential functional differences between the prokaryotic-like genome of the plastid and the eukaryotic genome of the plant cell nucleus will have an impact on characteristics of transgenic organism. Thus, the main goals are to generate transgenic plants with the traits of interest as well as minimizing the amount of transgenic DNA in plants while maximizing stability of gene expression and trait performance. In this review, two broad groups of gene delivery methods will be discussed namely, (bilogical and physical methods) and subsequently there applications for improving disease resistance will be discussed.

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Improvement of the Aluminum Borate Whisker-Mediated Method of DNA Delivery into Rice Callus

Cited by 19 publications

References 17 publications

Silicon Carbide Whisker-mediated Plant Transformation

Silicon Carbide Whisker-mediated Plant Transformation

Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease

Novel Techniques for Gene Delivery into Plants and Its Applications for Disease Resistance in Crops

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