Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease

Hassan, Mahmood Ul; Akram, Zahid; Ali, Shaukat; Ali, Ghulam Muhammad; Zafar, Yusuf; Shah, Zahid Hussain; Alghabari, Fahad

doi:10.1590/1984-70332016v16n2a17

Cited by 17 publications

(15 citation statements)

References 27 publications

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“…Notably, U 4-7-5 seeds exhibited upregulation of multiple transcripts and their homologs involved in the fungus cell wall degradation i.e., endochitinase 3, endochitinase-like, endoglucanase 17, beta-galactosidase 3/12/46. Furthermore, resistance to fungal disease in crop plants is enhanced by the expression of fungus cell wall catabolizing gene chitinase in groundnut [ 65 ], tomato [ 66 ] and other important crops.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Identified Coordinated Control of Key Pathways Regulating Cellular Physiology and Metabolism upon Aspergillus flavus Infection Resulting in Reduced Aflatoxin Production in Groundnut

Soni

Nayak

Kumar

et al. 2020

JoF

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Aflatoxin-affected groundnut or peanut presents a major global health issue to both commercial and subsistence farming. Therefore, understanding the genetic and molecular mechanisms associated with resistance to aflatoxin production during host–pathogen interactions is crucial for breeding groundnut cultivars with minimal level of aflatoxin contamination. Here, we performed gene expression profiling to better understand the mechanisms involved in reduction and prevention of aflatoxin contamination resulting from Aspergillus flavus infection in groundnut seeds. RNA sequencing (RNA-Seq) of 16 samples from different time points during infection (24 h, 48 h, 72 h and the 7th day after inoculation) in U 4-7-5 (resistant) and JL 24 (susceptible) genotypes yielded 840.5 million raw reads with an average of 52.5 million reads per sample. A total of 1779 unique differentially expressed genes (DEGs) were identified. Furthermore, comprehensive analysis revealed several pathways, such as disease resistance, hormone biosynthetic signaling, flavonoid biosynthesis, reactive oxygen species (ROS) detoxifying, cell wall metabolism and catabolizing and seed germination. We also detected several highly upregulated transcription factors, such as ARF, DBB, MYB, NAC and C2H2 in the resistant genotype in comparison to the susceptible genotype after inoculation. Moreover, RNA-Seq analysis suggested the occurrence of coordinated control of key pathways controlling cellular physiology and metabolism upon A. flavus infection, resulting in reduced aflatoxin production.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Identified Coordinated Control of Key Pathways Regulating Cellular Physiology and Metabolism upon Aspergillus flavus Infection Resulting in Reduced Aflatoxin Production in Groundnut

Soni

Nayak

Kumar

et al. 2020

JoF

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“…In addition, transgenic can also promote peanut breeding. Hassan et al (2016) transformated chitinase gene into peanut to enhance resistance to leaf spot. A RIL population was also used for the initial location of QTLs for peanut TFC by Mondal et al (2015), but further research on this topic is required.…”

Section: Hou Et Almentioning

confidence: 99%

Evaluation of total flavonoid content and analysis of related EST-SSR in Chinese peanut germplasm

Hou

Zhang

Cui

et al. 2017

Crop Breed. Appl. Biotechnol.

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“…This method was successfully used in maize and rice wherein either silicon carbide or aluminum borate-based whiskers were used to deliver the binary vector plasmids into the plant cells [43], [44]. This method was also used in groundnut transformation for inserting chitinase gene into epicotyl explants for the purpose generating leaf spot disease resistant plants [45].…”

Section: Development Of Transgenic Plant: Basic Concept and Methodsmentioning

confidence: 99%

Transgenic approaches for genetic improvement in groundnut (Arachis hypogaea L.) against major biotic and abiotic stress factors

Gantait

Mondal

2018

Journal of Genetic Engineering and Biotechnology

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Cultivated groundnut ( Arachis hypogaea L.) is considered as one of the primary oilseed crops and a major fodder for cattle industry in most of the developing countries, owing to its rich source of protein. It is due to its geocarpic nature of growth that the overall yield performance of groundnut is hindered by several biotic and abiotic stress factors. Multidimensional attempts were undertaken to combat these factors by developing superior groundnut varieties, modified with integral mechanism of tolerance/resistance; however this approach proved to be futile, owing to inferior pod and kernel quality. As a superior alternative, biotechnological intervention like transformation of foreign genes, either directly (biolistic) or via Agrobacterium , significantly aided in the development of advanced groundnut genotypes equipped with integral resistance against stresses and enhanced yield attributing traits. Several genes triggered by biotic and abiotic stresses, were detected and some of them were cloned and transformed as major parts of transgenic programmes. Application of modern molecular biological techniques, in designing biotic and abiotic stress tolerant/resistant groundnut varieties that exhibited mechanisms of resistance, relied on the expression of specific genes associated to particular stress. The genetically transformed stress tolerant groundnut varieties possess the potential to be employed as donor parents in traditional breeding programmes for developing varieties that are resilient to fungal, bacterial, and viral diseases, as well as to draught and salinity. The present review emphasizes on the retrospect and prospect of genetic transformation tools, implemented for the enhancement of groundnut varieties against key biotic and abiotic stress factors.

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Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease

Abstract: Peanut (Arachis hypogaea) is an important legume and oilseed crop,

Cited by 17 publications

References 27 publications

Transcriptome Analysis Identified Coordinated Control of Key Pathways Regulating Cellular Physiology and Metabolism upon Aspergillus flavus Infection Resulting in Reduced Aflatoxin Production in Groundnut

Transcriptome Analysis Identified Coordinated Control of Key Pathways Regulating Cellular Physiology and Metabolism upon Aspergillus flavus Infection Resulting in Reduced Aflatoxin Production in Groundnut

Evaluation of total flavonoid content and analysis of related EST-SSR in Chinese peanut germplasm

Transgenic approaches for genetic improvement in groundnut (Arachis hypogaea L.) against major biotic and abiotic stress factors

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