2000
DOI: 10.1590/s0034-71082000000300010
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Improvement of silver impregnation technique (Protargol) to obtain morphological features of protists ciliates, flagellates and opalinates

Abstract: The research on ciliates, flagelates and opalinates have been widespread by the utilization of techniques employing silver impregnation (Protargol), modified by several authors. However, these are time consuming and its results are variable. The present work is a variant of the technique described by Tuffrau (1964Tuffrau ( , 1967 showing some adaptations made in our laboratory. The organisms can be preserved by different fixatives (alcoholic Bouin, Stieve's fluid, 2.5% glutaraldehyde and others) and then rinse… Show more

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Cited by 13 publications
(8 citation statements)
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References 11 publications
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“…To identify the protozoa and for a photomicrographic record, all the cultures were periodically examined by using phase-contrast, differential-interference-contrast and bright-field microscopes (Nikon Labophot-2 with Nikon Digital Sight DS-2Mv and Nikon FX-35DX incorporated camera; Zeiss Axioskop 2 plus with Zeiss AxioCam MRC system). Diagnostic characters were studied with staining and impregnation techniques such as Harris hematoxylin, Klein, protargol, and butanolnigrosine (Borror, 1969;Kudo, 1971, p. 863;Lee et al, 1985;Silva-Neto, 2000).…”
Section: Methodsmentioning
confidence: 99%
“…To identify the protozoa and for a photomicrographic record, all the cultures were periodically examined by using phase-contrast, differential-interference-contrast and bright-field microscopes (Nikon Labophot-2 with Nikon Digital Sight DS-2Mv and Nikon FX-35DX incorporated camera; Zeiss Axioskop 2 plus with Zeiss AxioCam MRC system). Diagnostic characters were studied with staining and impregnation techniques such as Harris hematoxylin, Klein, protargol, and butanolnigrosine (Borror, 1969;Kudo, 1971, p. 863;Lee et al, 1985;Silva-Neto, 2000).…”
Section: Methodsmentioning
confidence: 99%
“…They are the best-studied of the non-pathogenic protists and have an established place in the laboratory where they are fairly easily manipulated [13]. They are large enough to image easily with light microscopy and are amenable to routine cytological staining [13,18,19,20]. Staining of surface features for confocal microscopy is a known art, and a functional method was published by Ann Fleury [14,15], Fleury and Laurent [6] and Fleury et al [17].…”
Section: Ciliated Protistsmentioning
confidence: 99%
“…Taxonomical and ecological studies on ciliated protozoa have been performed using a number of methods, including live observation, silver carbonate impregnation, scanning electron microscopy, and supravital staining, protargol, and dry and wet silver nitrate impregnation. These techniques have been modified and improved in several studies Lwoff 1930, 1936;Tuffrau 1964Tuffrau , 1967Wilbert 1975Wilbert , 1976Fernandez-Galiano 1976;Foissner 1976Foissner , 1991Foissner , 2014Dieckmann 1995;Silva-Neto 2000;Ma et al 2003;Vdacny and Foissner 2012). Among these methods, protargol impregnation is a key approach used to identify and describe ciliate species (Corliss 1979;Lynn 2008;Foissner 2014).…”
Section: Introductionmentioning
confidence: 99%