Improvement of productivity and polysaccharide-protein complex in Agaricus blazei

Chu, Jiunn-Nan; Young, Chiu-Chung; Tan, Chen-Chung; Wu, Shih Hsiung; Young, Li-Sen

doi:10.1590/s0100-204x2012000100013

Cited by 10 publications

(18 citation statements)

References 16 publications

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“…Harvesting time of the fruiting body took place when the mushroom reached its highest biomass, which occurs during the immature stage, when the veil membrane is enclosed and the gills are intact (Mendonça et al, 2005;Pokhrel & Ohga, 2007;Chu et al, 2012). Total mushroom fresh yield (g per pot) and PSPC content were measured from the first mushroom flush.…”

Section: Methodsmentioning

confidence: 99%

“…Pharmacological studies have shown that bioactive substances, such as polysaccharide-protein complexes (PSPC) from A. blazei, function as antioxidants, antimutagenics, antitumorigenics, and anticancer agents (Kimura et al, 2004;Firenzuoli et al, 2008). However, the harvesting time of A. blazei is relatively long (approximately 60 days) when compared to other related Agaricus sp., including A. bisporus (approximately 30 days) (Pardo-Gimenez et al, 2010;Chu et al, 2012). Attempts to improve A. blazei productivity have mainly focused on the application of different combinations of culture media and casing soils (Pardo-Gimenez et al, 2010;Chu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…However, the harvesting time of A. blazei is relatively long (approximately 60 days) when compared to other related Agaricus sp., including A. bisporus (approximately 30 days) (Pardo-Gimenez et al, 2010;Chu et al, 2012). Attempts to improve A. blazei productivity have mainly focused on the application of different combinations of culture media and casing soils (Pardo-Gimenez et al, 2010;Chu et al, 2012). Young et al (2012) studied six A. blazei-associated soil bacteria and successfully identified several beneficial microbes that could significantly improve A. blazei productivity and PSPC content.…”

Section: Introductionmentioning

confidence: 99%

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Cultivable mushroom growth-promoting bacteria and their impact on Agaricus blazei productivity

Young

Chu

Hameed

et al. 2013

Pesq. agropec. bras.

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-The objective of this work was to identify growth-promoting bacteria isolated from Agaricus blazei and to evaluate their effect on mushroom mycelial growth and productivity. A total of 56 A. blazei-associated bacterial isolates were obtained from casing soil and identified by 16S rRNA gene sequencing. Bacteria were evaluated as to phosphate-solubilization ability, nitrogen-fixation capability, and secretion of cellulase. Superior isolates were tested for their to effect on A. blazei productivity, micelial growth, and on the contents of the polysaccharide-protein complex and of N, P, K, Ca, and Mg. Bacterial isolates were identified as actinobacteria (60%), firmicutes (20%), and proteobacteria (20%). Among them, ten isolates had phosphate-solubilization ability, eight showed nitrogen-fixation capability, and 12 isolates promoted A. blazei mycelium growth. Bacterial inoculation reduces time till harvest in up to 26 days, increases fresh mushroom yield up to 215%, and increases total polysaccharide-protein complex content twofold when compared to the non-inoculated control. The actinobacteria group is the predominant A. blazei-associated phylum.Index terms: Agaricus blazei, bioprospecting, fresh mushroom yield, polysaccharide-protein complex. Termos para indexação: Agaricus blazei, bioprospecção, produção de cogumelos frescos, complexo polissacarídeo-proteínas. Bactérias cultiváveis promotoras do crescimento do cogumelo e seu impacto sobre a produtividade de Agaricus blazei

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cultivable mushroom growth-promoting bacteria and their impact on Agaricus blazei productivity

Young

Chu

Hameed

et al. 2013

Pesq. agropec. bras.

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“…Conventional culturing of ABM requires the initial spawning of the mycelium on a solid culture medium (15 to 21 days), and subsequent covering with suitable casing soil to stimulate fruiting-body formation, which normally takes around 45 to 60 days (Colauto et al, 2010;Chu et al, 2012). The time required to culture ABM from spawning to a harvestable mushroom size is long, compared to related Agaricus sp., such as A. bisporus (25 to 35 days, Pardo-Gimenez et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…bras., Brasília, v.47, n.6, p.815-821, jun. 2012 energy supply -for ABM cultures are often in the range of 40-70% (Mendonça et al, 2005;Donini et al, 2006;Andrade et al, 2007;Pokhrel & Ohga, 2007;Colauto et al, 2010;Chu et al, 2012). As such, it would be beneficial to identify techniques to decrease the time and energy required to culture ABM.…”

Section: Introductionmentioning

confidence: 99%

Beneficial bacterial strains on Agaricus blazei cultivation

Young

Chu

Young

2012

Pesq. agropec. bras.

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-The objective of this work was to evaluate the effects of six bacterial strains isolated from Agaricus blazei (ABM) on its cultivation. The six strains were characterized as to their effects on the productivity, polysaccharide-protein complex (PSPC), and polysaccharide contents of ABM cultured on sterilized casing soils. Three isolates enhanced ABM mycelium growth. Inoculation of Arthrobacter sp. or Exiguobacterium sp. on sterile peat casing soil resulted in 64% increase in ABM mushroom total fresh matter yield compared to the uninoculated control. Inoculation of Exiguobacterium sp., Microbacterium esteraromaticum or Pseudomonas resinovorans on sterilized loamy casing soil resulted in 62, 95, and 59% increase in ABM mushroom total fresh matter yield, respectively. The PSPC content in ABM increased 7 to 10% in casing soil inoculated with five of the six isolates compared to the uninoculated control. Exiguobacterium sp. inoculated on casing soil resulted in a mushroom-polysaccharide content 15% higher than the control. Moreover, inoculation of five of the six isolates on the casing soil reduced the harvesting time from 10 to 27 days. The evaluated beneficial microbes improve the yield, PSPC, and polysaccharide contents, besides reducing the harvesting time in ABM culture.Index terms: Agaricus blazei, beneficial microbe, casing soil, mushroom productivity, polysaccharide-protein complex, probiotics. Estirpes bacterianas benéficas ao cultivo de Agaricus blazeiResumo -O objetivo deste trabalho foi avaliar os efeitos de seis estirpes bacterianas isoladas de Agaricus blazei (ABM) sobre o seu cultivo. As seis estirpes foram caracterizadas quanto a seus efeitos sobre a produtividade, o conteúdo do complexo polissacarídeo-proteína (CPP) e o conteúdo de polissacarídeos, em ABM cultivado em solos esterilizados. Três isolados aumentaram o crescimento micelial de ABM. A inoculação de Arthrobacter sp. ou Exiguobacterium sp. em cobertura de turfa estéril resultou em aumento de 64% na produção total de cogumelos, em comparação ao controle sem inoculação. A inoculação de Exiguobacterium sp., Microbacterium esteraromaticum ou Pseudomonas resinovorans em solo argiloso esterilizado resultou em 62, 95, e 59% de aumento na produção total de cogumelos, respectivamente. O conteúdo de CPP em ABM aumentou de 7 a 10% em solo com inoculação de cinco dos seis isolados, em comparação ao controle sem inoculação. A inoculação de Exiguobacterium sp. em terra de cobertura resultou em conteúdo de polissacarídeo nos cogumelos 15% superior ao do controle. Além disso, a inoculação de cinco dos seis isolados na terra de cobertura reduziu o tempo de colheita de 10 a 27 dias. Os microrganismos benéficos avaliados melhoram a produção e os conteúdos de CPP e de polissacarídeos, além de reduzir o tempo de colheita no cultivo de ABM.Termos para indexação: Agaricus blazei, microrganismo benéfico, terra de cobertura, produtividade de cogumelos, complexo polissacarídeo-proteína, probiótico.

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Overview of four Agaricus subrufescens strains used in the last 15 years in Brazil and other countries and current potential materials for the future

et al. 2021

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Improvement of productivity and polysaccharide-protein complex in Agaricus blazei

Cited by 10 publications

References 16 publications

Cultivable mushroom growth-promoting bacteria and their impact on Agaricus blazei productivity

Cultivable mushroom growth-promoting bacteria and their impact on Agaricus blazei productivity

Beneficial bacterial strains on Agaricus blazei cultivation

Overview of four Agaricus subrufescens strains used in the last 15 years in Brazil and other countries and current potential materials for the future

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