Beneficial bacterial strains on Agaricus blazei cultivation

Young, Li-Sen; Chu, Jiunn-Nan; Young, Chiu-Chung

doi:10.1590/s0100-204x2012000600012

Cited by 13 publications

(13 citation statements)

References 22 publications

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“…The physicochemical properties of the culture medium and loamy casing soil were previously described in Young et al (2012) and are summarized in Table 1. The casing soil was soaked in sterile water at a ratio of 1:1 (w/w) and shook for 0.5 hour at 25 o C. Aliquots (100 ml) of the soil solution were plated on nutrient agar (HiMedia Laboratories, Mumbai, India) by the serial dilution method, and incubated at 25 o C for three days.…”

Section: Methodsmentioning

confidence: 99%

“…Amplification was set at the following conditions: 95 o C for 5 min, followed by 35 cycles of 95 o C for 1 min, 60 o C for 1 min, 72 o C for 2 min, and final extension at 72 o C for 7 min. DNA fragments of approximately 1,500 bp were purified using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) and were sequenced as described in Young et al (2012). The cycle sequencing primers used were: 3F: 5'-CCTACGGGAGGCAGCAG-3', 4R: 5'-TTA CCGCGGCTGCTGGCAC-3', and 5F: 5'-AAACTC AAATGAATTGACGGGG-3' (Edwards et al, 1989).…”

Section: Methodsmentioning

confidence: 99%

“…Attempts to improve A. blazei productivity have mainly focused on the application of different combinations of culture media and casing soils (Pardo-Gimenez et al, 2010;Chu et al, 2012). Young et al (2012) studied six A. blazei-associated soil bacteria and successfully identified several beneficial microbes that could significantly improve A. blazei productivity and PSPC content. Therefore, it would be beneficial to characterize mushroom-associated microorganisms and to identify biological agents that can be used to decrease the time and energy required to culture A. blazei.…”

Section: Introductionmentioning

confidence: 99%

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Cultivable mushroom growth-promoting bacteria and their impact on Agaricus blazei productivity

Young

Chu

Hameed

et al. 2013

Pesq. agropec. bras.

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-The objective of this work was to identify growth-promoting bacteria isolated from Agaricus blazei and to evaluate their effect on mushroom mycelial growth and productivity. A total of 56 A. blazei-associated bacterial isolates were obtained from casing soil and identified by 16S rRNA gene sequencing. Bacteria were evaluated as to phosphate-solubilization ability, nitrogen-fixation capability, and secretion of cellulase. Superior isolates were tested for their to effect on A. blazei productivity, micelial growth, and on the contents of the polysaccharide-protein complex and of N, P, K, Ca, and Mg. Bacterial isolates were identified as actinobacteria (60%), firmicutes (20%), and proteobacteria (20%). Among them, ten isolates had phosphate-solubilization ability, eight showed nitrogen-fixation capability, and 12 isolates promoted A. blazei mycelium growth. Bacterial inoculation reduces time till harvest in up to 26 days, increases fresh mushroom yield up to 215%, and increases total polysaccharide-protein complex content twofold when compared to the non-inoculated control. The actinobacteria group is the predominant A. blazei-associated phylum.Index terms: Agaricus blazei, bioprospecting, fresh mushroom yield, polysaccharide-protein complex. Termos para indexação: Agaricus blazei, bioprospecção, produção de cogumelos frescos, complexo polissacarídeo-proteínas. Bactérias cultiváveis promotoras do crescimento do cogumelo e seu impacto sobre a produtividade de Agaricus blazei

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cultivable mushroom growth-promoting bacteria and their impact on Agaricus blazei productivity

Young

Chu

Hameed

et al. 2013

Pesq. agropec. bras.

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“…For A. subrufescens cultivation, the use of inoculants on casing layer has been reported (Young et al, 2012) but the present work is the first to report the utilization of inoculants for the composting process aiming a simpler technology which may be apropriate for small mushroom growers in developing countries.…”

Section: Resultsmentioning

confidence: 87%

Microbial inoculation during composting improves productivity of sun mushroom (Agaricus subrufescens Peck)

Figueirêdo¹,

Martos²,

Siqueira³

et al. 2013

Afr. J. Biotechnol.

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The aim of this research was to evaluate the application of different microbial additives during composting, on some parameters of the production of Agaricus subrufescens. Compost was prepared over two weeks with ammonia assimilating bacterial and a thermophillic fungus as microbiological additives. These additives were introduced during two week composting to promote greater selectivity of the substrate cultivation and provide increased productivity of mushrooms. The data shows that the microbiological additives used in composting had a significantly higher productivity, when compared to treatments without additives. These species can be used as microbiological additives in A. subrufescens cultivation.

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“…It has been reported that Ochrobactrum pseudogrignonense is able to induce and increase mushroom growth of Pleurotus ostreatus with significant increasing yield (Maryana and Aryantha 2013). Young et al (2012) found six soil bacterial strain that increases Agaricus blazei growth. Studies regarding biogical factors such as induction by indigenous MGPB are rarely done.…”

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confidence: 99%

Potential MGPB In Optimizing Paddy Straw Mushroom (Volvariella volvacea WW-08) Growth

JEMSI¹,

Aryantha²

2017

Microbiol Indones

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Stable environment in Indonesia all year is good for mushroom growth (Aryantha 2005). Many mushrooms in Indonesia are often used as food, one of which is Volvariella volvacea (V. volvacea) . V. volvacea has distinct and delicious flavor, soft, elastic, makes it highly demand . Demand of V. Volvacea is high approximately 50-60% of total national production, with average of elevated demand was 20-25%/year (MAJI 2007). Production of V. volvacea peak at 70% that was from West Java, Middle Java, East Java, and Lampung (Tridjaja 2005; Sumiati and Djuariah 2007). In 2015, mushroom demands gradually increase that peaked 17500 ton/year, whereas only 13825 ton/year has been fullfiled (MAJI 2007). Obstacles commonly encountered during production of V. volvacea is decreased yields due to high contamination (Sinaga 2012). Approach increasing growth using Mushroom Growth Promoting Bacteria (MGPB) are therefore needed. Young et al. (2013) found bacteria that produces metabolite in growth medium of Agaricus blazei in dissolving metal, calcium, and nitrogen fixation that Paddy straw mushroom (Volvariella volvacea) contains high protein content and delicious flavor, makes it highly demand each year. Production of V. volvacea does not merit the requirements due to its limited production. Therefore, approach in increasing production using Mushroom Growth Promoting Bacteria (MGPB) are needed. This study aims to obtain MGPB isolate as potential agent to increase V. volvacea strain WW-08 growth. This is experimental research in laboratory that consisted of indigenous bacteria isolation, MGPB screening with dual culture, MGPB inoculum optimization, molecular identification of selected MGPB using 16S rRNA, protein profiling with SDS-PAGE, and fruting body production. Indigenous bacteria obtained Key words: Bacillus cereus, Mushroom Growth Promoting Bacteria (MGPB), Volvariella volvacea WW-08Jamur merang (Volvariella volvacea) mengandung protein tinggi dan rasa yang lezat, yang menyebabkan permintaan meningkat setiap tahunnya. Produksi V. volvacea ini sangat terbatas sehingga tidak mencukupi kebutuhan di masyarakat. Oleh karena itu salah satu cara yang dilakukan dalam meningkatkan produksi dengan menggunakan pendekatan Mushroom Growth Promoting Bacteria (MGPB). Penelitian ini bertujuan untuk mendapatkan isolat MGPB sebagai agen potensial untuk meningkatkan pertumbuhan V. volvacea strain WW-08. Penelitian ini merupakan penelitian eksperimental di laboratorium yang terdiri dari isolasi bakteri, penyaringan MGPB dengan metode dual culture, optimasi inokulum MGPB, identifikasi molekuler MGPB terpilih dengan menggunakan 16S rRNA, profil protein dengan menggunakan SDS-PAGE, dan produksi tubuh buah jamur. Bakteri yang diperoleh dari hasil isolasi media pertumbuhan sebanyak 58 isolat, dan bakteri W34 pada

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Beneficial bacterial strains on Agaricus blazei cultivation

Cited by 13 publications

References 22 publications

Cultivable mushroom growth-promoting bacteria and their impact on Agaricus blazei productivity

Cultivable mushroom growth-promoting bacteria and their impact on Agaricus blazei productivity

Microbial inoculation during composting improves productivity of sun mushroom (Agaricus subrufescens Peck)

Potential MGPB In Optimizing Paddy Straw Mushroom (Volvariella volvacea WW-08) Growth

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