Improvement of poly-γ-glutamic acid biosynthesis in a moving bed biofilm reactor by Bacillus subtilis NX-2

Jiang, Yun; Tang, Bao Quoc; Xu, Zhihua; Liu, Kun; Xu, Zheng; Feng, Xiaohai; Xu, Hong

doi:10.1016/j.biortech.2016.06.103

Cited by 29 publications

(13 citation statements)

References 34 publications

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“…Several strategies have been made to improve γ-PGA production and productivity shown in Table 4. B. subtilis NX-2 had the highest yield of γ-PGA (74.2 g/L) with a productivity of 1.24 g/L/h [43]. However, their γ-PGA productivity was much lower than the value of 1.85 g/L/h observed in the present study.…”

Section: Repeated Fed-batch Fermentation For γ-Pga Production By B Scontrasting

confidence: 83%

“…To investigate the long-term stability and maximize the γ-PGA production, repeated fed-batch fermentations were done in this study. This approach has been widely used to improve the productivity of γ-PGA because it reduces the delay period of the strains [40,43]. Based on the fed-batch results, we performed repeated fed-batch fermentations of B. siamensis IR10 for six cycles each 24 h long (Fig.…”

Section: Repeated Fed-batch Fermentation For γ-Pga Production By B Smentioning

confidence: 99%

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High-level production of poly-γ-glutamic acid from untreated molasses by Bacillus siamensis IR10

et al. 2020

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Background: Poly-γ-glutamic acid (γ-PGA) is a promising biopolymer and has been applied in many fields. Bacillus siamensis SB1001 was a newly isolated poly-γ-glutamic acid producer with sucrose as its optimal carbon source. To improve the utilization of carbon source, and then molasses can be effectively used for γ-PGA production, 60 cobalt gamma rays was used to mutate the genes of B. siamensis SB1001. Results: Bacillus siamensis IR10 was screened for the production of γ-PGA from untreated molasses. In batch fermentation, 17.86 ± 0.97 g/L γ-PGA was obtained after 15 h, which is 52.51% higher than that of its parent strain. Fed-batch fermentation was performed to further improve the yield of γ-PGA with untreated molasses, yielding 41.40 ± 2.01 g/L of γ-PGA with a productivity of 1.73 ± 0.08 g/L/h. An average γ-PGA productivity of 1.85 g/L/h was achieved in the repeated fed-batch fermentation. This is the first report of such a high γ-PGA productivity. The analysis of the enzyme activities showed that they were affected by the carbon sources, enhanced ICDH and GDH, and decreased ODHC, which are important for γ-PGA production. Conclusion: These results suggest that untreated molasses can be used for economical and industrial-scale production of γ-PGA by B. siamensis IR10.

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Section: Repeated Fed-batch Fermentation For γ-Pga Production By B Scontrasting

confidence: 83%

Section: Repeated Fed-batch Fermentation For γ-Pga Production By B Smentioning

confidence: 99%

High-level production of poly-γ-glutamic acid from untreated molasses by Bacillus siamensis IR10

et al. 2020

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“…Increasing the culture temperature at the beginning of fermentation allows cells to grow stronger and faster by consuming substrates, thereby shortening the fermentation period. On the other hand, improvement in substrate uptake could provide more precursors and energy for cell growth and product synthesis, which is consistent with other studies [ 25 ]. Moreover, energy consumption can be reduced during the early stage of fermentation due to the elevated temperature, which will be beneficial for the industrialized production of HSAF in the future.…”

Section: Discussionsupporting

confidence: 92%

Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11

et al. 2018

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BackgroundHeat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has reported on the fermentation process involved, representing huge obstacles for large-scale industrial production.ResultsMedium capacity, culture temperature, and fermentation time were identified as the most significant factors affecting the production of HSAF and employed for further optimization through statistical methods. Based on the analysis of kinetic parameters at different temperatures, a novel two-stage temperature control strategy was developed to improve HSAF production, in which the temperature was increased to 32 °C during the first 12 h and then switched to 26 °C until the end of fermentation. Using this strategy, the maximum HSAF production reached 440.26 ± 16.14 mg L− 1, increased by 9.93% than that of the best results from single-temperature fermentation. Moreover, the fermentation time was shortened from 58 h to 54 h, resulting in the enhancement of HSAF productivity (17.95%) and yield (9.93%).ConclusionsThis study provides a simple and efficient method for producing HSAF that could be feasibly applied to the industrial-scale production of HSAF.Electronic supplementary materialThe online version of this article (10.1186/s12896-018-0478-2) contains supplementary material, which is available to authorized users.

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“…Furthermore, it is well documented that glucose, being a simple carbon source, is preferred by many microbes (Jiang et al . ), and it is cheap and suitable for large‐scale production. Thus, glucose was chosen as the most suitable carbon source for HSAF fermentation.…”

Section: Resultsmentioning

confidence: 99%

Enhanced heat stable antifungal factor production by Lysobacter enzymogenes OH11 with cheap feedstocks: medium optimization and quantitative determination

Tang

Zhao

Shi

et al. 2018

Lett Appl Microbiol

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Significance and Impact of the Study: Heat stable antifungal factor (HSAF) exhibits a potent and broad antifungal activity with a novel mode of action. Increased production and reduced cost of raw materials are particularly important for the future production of HSAF, however, no report was involved in these studies. This study aimed to improve the production of HSAF with cheap raw materials through the medium optimization, which would lay the foundation for the application of HSAF in biological control.

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Improvement of poly-γ-glutamic acid biosynthesis in a moving bed biofilm reactor by Bacillus subtilis NX-2

Cited by 29 publications

References 34 publications

High-level production of poly-γ-glutamic acid from untreated molasses by Bacillus siamensis IR10

High-level production of poly-γ-glutamic acid from untreated molasses by Bacillus siamensis IR10

Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11

Enhanced heat stable antifungal factor production by Lysobacter enzymogenes OH11 with cheap feedstocks: medium optimization and quantitative determination

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