Enhanced heat stable antifungal factor production by <i>Lysobacter enzymogenes </i>
OH11 with cheap feedstocks: medium optimization and quantitative determination

Tang, Bao Quoc; Zhao, Yangyang; Shi, Xiaofeng; Xu, Heng; Dai, Chuan‐Chao; Liu, F.‐Q.

doi:10.1111/lam.12870

Cited by 18 publications

(18 citation statements)

References 23 publications

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“…oryzae RS105. In L. enzymogenes , the yield of HSAF accumulates with growth and reaches a maximum concentration at the stationary phase (Tang et al, 2018). In addition, the biosynthesis of HSAF is regulated by the DSF quorum sensing system in L. enzymogenes , and the loss of the key DSF quorum sensing system genes leads to a significant decrease in HSAF production (Han et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

The AHL Quorum-Sensing System Negatively Regulates Growth and Autolysis in Lysobacter brunescens

Ling

Zhou

et al. 2019

Front. Microbiol.

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Lysobacter species are emerging as novel sources of antibiotics, but the regulation of their physiological metabolism is still poorly understood. In this work, we extracted AHL (acyl-homoserine lactone) autoinducers, identified the structures of AHLs and described the AHL quorum-sensing system in Lysobacter brunescens OH23. AHLs were isolated from the supernatant of L. brunescens OH23, and ESI-MS/MS (electrospray ionization mass spectrometry) analysis revealed biosynthesis of three different AHL chemical structures by L. brunescens OH23: N-(3-oxohexanoyl)- homoserine lactone (HSL), 3-OH-C10-HSL and C8-HSL. The growth rate of AHL quorum-sensing knockout mutants was dramatically increased compared to that of wildtype. Sucrose consumptions were also twice as high in AHL quorum-sensing knockout mutants than that in wildtype in early-log phase. Additionally, expression of key genes related to sucrose metabolism α-glucosidase was enhanced in AHL quorum-sensing knockout mutants, which indicated that AHL quorum sensing negatively regulates sucrose uptake and metabolism which further affects the growth rate of L. brunescens. Furthermore, autolysis was strongly induced in AHL quorum-sensing knockout mutants compared to wildtype, suggesting that AHL quorum sensing plays a negative regulatory role in cell autolysis. Moreover, compared to wildtype, XSAC (Xanthomonas-specific antibiotic compound) production was significantly increased in AHL knockout mutants in the early-log and late-log phases, and surface motility capabilities are also enhanced also in AHL knockout mutants; the normalized data of XSAC production and surface motility and expressions of key genes related to these two phenotypes reveal that growth rare and autolysis strongly affects XSAC biosynthesis and surface motility rather than AHL quorum-sensing system. Our results show that the AHL quorum-sensing system negatively regulates cell growth and autolysis, and further maintain nutrition homeostasis and population stability in L. brunescens.

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Section: Resultsmentioning

confidence: 99%

The AHL Quorum-Sensing System Negatively Regulates Growth and Autolysis in Lysobacter brunescens

Ling

Zhou

et al. 2019

Front. Microbiol.

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“…Because of its significant bioactivities and distinct structure, great efforts have been made to investigate the biosynthetic and regulatory mechanisms of HSAF 8, 9, 11 . In our previous studies, strategies including genetic modification and fermentation optimization have been investigated in an effort to improve the production of HSAF in L. enzymogenes strain OH11 12‐15 . In addition to clarifying the biosynthetic and regulatory mechanisms of HSAF and improving its production, extended research is required to explore the application potential of HSAF for future exploitation as a new fungicide or antifungal drug.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol ability and action mechanism of dihydromaltophilin against Colletotrichum fructicola causing anthracnose of pear fruit

Tang

Cao

et al. 2020

Pest Management Science

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BACKGROUND Anthracnose caused by Colletotrichum fructicola is one of the most important diseases in pear fruit, resulting in huge economic losses. Public awareness of protecting the environment and food safety, together with pathogen resistance to many key fungicides have led to an urgent need to develop alternative strategies for controlling fruit diseases. Here, the antifungal activity of a natural product, dihydromaltophilin [heat‐stable antifungal factor (HSAF)], against C. fructicola in vitro and in vivo was investigated to determine its efficacy for anthracnose management. RESULTS HSAF exhibited pronounced antifungal activity against in vitro mycelial growth of C. fructicola, with a half‐inhibition concentration of 0.43 mg L−1. Hyphae treated with HSAF showed defects such as hyperbranching, swelling and depolarized growth. Conidia germination in the pathogen was inhibited by HSAF in a dose‐dependent manner. In the presence of 4 mg L−1 HSAF, conidia germination was significantly delayed, and germ tube growth was inhibited. HSAF at 8 mg L−1 completely blocked conidia germination in C. fructicola. In addition, HSAF disrupted coordination of cytokinesis with growth and nuclear division, induced reactive oxygen species production in conidia, and damaged the integrity of the conidia cell wall. Moreover, an in vivo test confirmed that 50 mg L−1 HSAF significantly reduced the development of anthracnose decay in pear fruit caused by C. fructicola. CONCLUSION HSAF was highly effective in reducing pear anthracnose caused by C. fructicola and has great potential to become a new type of fruit preservative.

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“…In recent years, the Gram-negative bacterium Lysobacter enzymogenes has emerged as a new biocontrol agent due to its prolific production of lytic enzymes and potent natural antibiotic products (Xie et al 2012;Xu et al 2015;Odhiambo et al 2017;Tang et al 2018;Yu et al 2018). Strain C3 of L. enzymogenes is an antifungal agent that exhibits a broad antimicrobial spectrum against fungal and oomycete pathogens (Li et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Protecting books from mould damage by decreasing paper bioreceptivity to fungal attack using decoloured cell‐free supernatant ofLysobacter enzymogenesC3

et al. 2019

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Aims To evaluate whether decoloured cell‐free supernatant of Lysobacter enzymogenes C3 can decrease paper bioreceptivity to fungal attack. Methods and Results To prepare colourless C3 supernatant, single‐factor design and uniform design were applied. The optimum conditions with high decolouration degree and low antifungal activity loss were achieved as follows: carbon granule content 1·6% (M/V), temperature 27°C, decolouring time 1·2 h and pH 8·0. An agar plate bioassay was used to assess the antifungal activity of the decoloured supernatant against the fungal isolates obtained from contaminated books, and strong suppression was observed. Small‐sacle laboratory test was further introduced, in which common book papers were artificially inoculated with the fungal isolates, and then sprayed uniformly with decoloured supernatant or water. The results showed that, after treatment, the paper showed a significantly low extent of fungal colonization and high tensile strength, and maintained the same colour before and after treatment. Conclusion These results suggest that the decoloured C3 supernatant inhibits fungal growth on types of paper commonly used in books. Significance and Impact of the Study Decoloured C3 supernatant could be used as a preventive agent to protect books and other paper‐based items against fungal growth.

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Enhanced heat stable antifungal factor production by Lysobacter enzymogenes OH11 with cheap feedstocks: medium optimization and quantitative determination

Cited by 18 publications

References 23 publications

The AHL Quorum-Sensing System Negatively Regulates Growth and Autolysis in Lysobacter brunescens

The AHL Quorum-Sensing System Negatively Regulates Growth and Autolysis in Lysobacter brunescens

Biocontrol ability and action mechanism of dihydromaltophilin against Colletotrichum fructicola causing anthracnose of pear fruit

Protecting books from mould damage by decreasing paper bioreceptivity to fungal attack using decoloured cell‐free supernatant ofLysobacter enzymogenesC3

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