The AHL Quorum-Sensing System Negatively Regulates Growth and Autolysis in Lysobacter brunescens

Ling, Jun; Zhou, Lan; Wu, Guichun; Zhao, Yunxia; Jiang, Tianping; Liu, Fengquan

doi:10.3389/fmicb.2019.02748

Cited by 18 publications

(12 citation statements)

References 46 publications

(62 reference statements)

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“…In this study, we detected the production of AHL in both strains after dilutions 1:125 and 1:3126 for the strains PEPV15 and PEPV16, respectively. In agreement with these results, we found in the genome of the strain PEPV16 a gene encoding a N-acyl-L-homoserine lactone (AHL) synthase and in that of strain PEPV15 a gene encoding a GNAT family N-acetyltransferase involved in AHL biosynthesis [54].…”

Section: Analysis Of Genes Involved In Quorum Sensing and Plant Colonizationsupporting

confidence: 84%

Connecting the Lab and the Field: Genome Analysis of Phyllobacterium and Rhizobium Strains and Field Performance on Two Vegetable Crops

et al. 2021

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The legume nodules are a rich source not only of rhizobia but also of endophytic bacteria exhibiting plant growth-promoting mechanisms with potential as plant biostimulants. In this work we analyzed the genomes of Phyllobacterium endophyticum PEPV15 and Rhizobium laguerreae PEPV16 strains, both isolated from Phaseolus vulgaris nodules. In silico analysis showed that the genomes of these two strains contain genes related to N-acyl-homoserine lactone (AHL) and cellulose biosynthesis, involved in quorum sensing and biofilm formation, which are essential for plant colonization. Several genes involved in plant growth promotion such as those related to phosphate solubilization, indole acetic acid production, siderophore biosynthesis and nitrogen fixation were also located in both genomes. When strains PEPV15 and PEPV16 were inoculated in lettuce and carrot in field assays, we found that both significantly increased the yield of lettuce shoots and carrot roots by more than 20% and 10%, respectively. The results of this work confirmed that the genome mining of genes involved in plant colonization and growth promotion is a good strategy for predicting the potential of bacterial strains as crops inoculants, opening new horizons for the selection of bacterial strains with which to design new, effective bacteria-based plant biostimulants.

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Section: Analysis Of Genes Involved In Quorum Sensing and Plant Colonizationsupporting

confidence: 84%

Connecting the Lab and the Field: Genome Analysis of Phyllobacterium and Rhizobium Strains and Field Performance on Two Vegetable Crops

et al. 2021

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“…The reason for autolysis in DSM 7029 is possibly the tight regulation and/or expression of lytic enzymes. The quorum sensing (QS) system and related regulators can modulate several cellular processes in diverse bacteria, such as cell growth and autolysis, in a cell density-dependent manner 38 , 56 – 59 . To explore the potential correlation between autolysis and the removed genes, we conducted single inactivation of sixteen hypothetical regulatory genes and putative lytic genes located in the deletion regions (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The comparison of other widely used chassis with the DSM 7029 strain is also summarized here (Supplementary Table 1 ). However, during our deep investigation of this strain to improve the production of epothilones, we found that the early autolysis of DSM 7029 led to most cell death after 48 h of fermentation, which also has been recently reported to occur in many Gram-negative bacteria 38 – 40 , severely affecting the biomass of DSM 7029 and ultimately restricting the heterologous production yields of desired compounds 41 . Sucrose addition to the medium delayed the cell death and extended the fermentation cycle 41 , circumventing this important negative factor in industrial fermentation production to some degree.…”

Section: Introductionmentioning

confidence: 80%

Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria

et al. 2021

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Heterologous expression of biosynthetic gene clusters (BGCs) avails yield improvements and mining of natural products, but it is limited by lacking of more efficient Gram-negative chassis. The proteobacterium Schlegelella brevitalea DSM 7029 exhibits potential for heterologous BGC expression, but its cells undergo early autolysis, hindering further applications. Herein, we rationally construct DC and DT series genome-reduced S. brevitalea mutants by sequential deletions of endogenous BGCs and the nonessential genomic regions, respectively. The DC5 to DC7 mutants affect growth, while the DT series mutants show improved growth characteristics with alleviated cell autolysis. The yield improvements of six proteobacterial natural products and successful identification of chitinimides from Chitinimonas koreensis via heterologous expression in DT mutants demonstrate their superiority to wild-type DSM 7029 and two commonly used Gram-negative chassis Escherichia coli and Pseudomonas putida. Our study expands the panel of Gram-negative chassis and facilitates the discovery of natural products by heterologous expression.

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“…[77]. Thus, modulation of genes related to secretion systems might result in gain/loss of ability to compete with other (micro)organisms [78]. In agreement with this statement, IND down-regulated T4SS and decreased antimicrobial activity in AZ78.…”

Section: Discussionmentioning

confidence: 80%

Listening to bacterial Esperanto: transcriptome reprogramming in a plant beneficial rhizobacterium

Bejarano

Perazzolli

Pertot

et al. 2021

Preprint

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Intraspecies, interspecies and interkingdom signalling occurring via diffusible communication signals (DCSs) continuously shapes the gene expression patterns of individual bacterial species in the rhizosphere, affecting bacterial functions within the rhizosphere microbial community. To unravel how DCSs influence rhizosphere competence of plant beneficial rhizobacteria, we carried out a functional and transcriptome analysis on the plant beneficial bacterium Lysobacter capsici AZ78 (AZ78). Results reveal that 13-methyltetradecanoic acid and indole, glyoxylic acid and 2,3-butanedione play a relevant role in the interaction between L. capsici members and other soil-living (micro)organisms. DCSs regulated mechanisms of multistress and multidrug tolerance and persistence, including detoxification, motility, antibiotic production, and expression of secretion systems. In particular, 13-methyltetradecanoic acid, glyoxylic acid and 2,3-butanedione might enable AZ78 to rapidly colonize the rhizosphere. Moreover, glyoxylic acid and 2,3-butanedione elicit biological responses to outcompete other (micro)organisms. In contrast, indole inactivates twitching motility and antibiotic production. These results demonstrate that DCSs influence the functioning of plant beneficial rhizobacteria and suggest that plant beneficial L. capsici strains could use them to foster rhizosphere colonization and enhance in vivo activities to increase soil health and plant fitness.

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The AHL Quorum-Sensing System Negatively Regulates Growth and Autolysis in Lysobacter brunescens

Cited by 18 publications

References 46 publications

Connecting the Lab and the Field: Genome Analysis of Phyllobacterium and Rhizobium Strains and Field Performance on Two Vegetable Crops

Connecting the Lab and the Field: Genome Analysis of Phyllobacterium and Rhizobium Strains and Field Performance on Two Vegetable Crops

Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria

Listening to bacterial Esperanto: transcriptome reprogramming in a plant beneficial rhizobacterium

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