Improvement of hydroxyvalerate fraction in poly(β-hydroxybutyrate-co-β-hydroxyvalerate) by a mutant strain of Alcaligenes eutrophus

Lee, In Young; Kim, Guk Jin; Choi, Dae Keon; Yeon, B K; Park, Young Hoon

doi:10.1016/0922-338x(96)82217-3

Cited by 19 publications

(8 citation statements)

References 7 publications

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“…Blockage of propionic acid oxidation pathways has also been applied to improve 3HV synthesis in R. eutropha, however the results were not presented or were far from the maximum theoretical value (Lee et al 1996). Together with its ability of growing and accumulating PHA directly from sucrose, the industrial use of B. sacchari mutant can reduce the production costs of P3HB-co-3HV when propionic acid is added as co-substrate.…”

Section: Resultsmentioning

confidence: 97%

“…3, values around US$ 1.00 per kg at Y 3HV/prop of 1.2 g g -1 can be reached (up to 73% reduction compared to Y 3HV/prop 0.1 g g -1 ), which are closer to the value to produce P3HB from sucrose (US$ 0.75 per kg). In addition, B. sacchari IPT 189 accumulated a large amount of P(3HB-co-3HV) in a relatively short period of time, similarly to the best performing microorganisms used in the technical production of these materials (Anderson and Dawes 1990;Lee et al 1996;Taciro et al 1997;Du et al 2001;Nonato et al 2001) being therefore a promising strain to be used in larger scales.…”

Section: Resultsmentioning

confidence: 97%

“…Du et al (2001) obtained Y 3HV/prop values of 0.30 and 0.40 g g -1 with R. eutropha. Lee et al (1996) using the mutant BK23 (R. eutropha) reached 0.6 g g -1 . Silva et al (2000) attained 1.20 g g -1 using the same B. sacchari mutant, feeding glucose and propionic acid by pH control (pHstat control) and 0.10 g g -1 using wild type.…”

Section: Resultsmentioning

confidence: 99%

“…1b, d). It has been pointed out in the literature that the increment on PHA productivity and the amount of polymer accumulated are fundamental features to decrease the polyhydroxyalkanoate production costs (Lee et al 1996;Choi and Lee 1999). In contrast, the 3HV content in the copolymer decreased from 10 mol % (/ c = 0.15 g g -1 h -1 ; s/p = 30:1) to about 6 mol% (both to / c 0.30 and 0.40 g g -1 ) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Procedures have been adopted to improve Y 3HV/prop, including the use of genetically modified bacteria (Byrom 1992;Lee et al 1996) and substrate flow optimization (Du et al 2001). Recently, the Burkholderia sacchari IPT 189 mutant was described as partially blocked in the propionic acid oxidation pathway (Silva et al 2000;Brämer et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) with a broad range of 3HV content at high yields by Burkholderia sacchari IPT 189

Rocha

Silva

Taciro

et al. 2007

World J Microbiol Biotechnol

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The biosynthesis of poly(3-hydroxybutyrateco-3-hydroxyvalerate) from sucrose and propionic acid by Burkholderia sacchari IPT 189 was studied using a twostage bioreactor process. In the first stage, this bacterium was cultivated in a balanced culture medium until sucrose exhaustion. In the second stage, a solution containing sucrose and propionic acid as carbon source was fed to the bioreactor at various sucrose/propionic acid (s/p) ratios at a constant specific flow rate. Copolymers with 3HV content ranging from 40 down to 6.5 (mol%) were obtained with 3HV yield from propionic acid (Y 3HV/prop ) increasing from 1.10 to 1.34 g g -1 . Copolymer productivity of 1 g l -1 h -1 was obtained with polymer biomass content rising up to 60% by increasing a specific flow rate at a constant s/p ratio. Increasing values of 3HV content were obtained by varying the s/p ratios. A simulation of production costs considering Y 3HV/prop obtained in the present work indicated that a reduction of up to 73% can be reached, approximating US$ 1.00 per kg which is closer to the value to produce P3HB from sucrose (US$ 0.75 per kg).Keywords Biodegradable polyester Á Burkholderia sacchari Á P(3HB-co-3HV) Á Propionic acid Á Sucrose Á Y 3HV/prop

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) with a broad range of 3HV content at high yields by Burkholderia sacchari IPT 189

Rocha

Silva

Taciro

et al. 2007

World J Microbiol Biotechnol

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Fermentative Production of SCL Polyhydroxyalkanoates (SCL‐PHAs)

Lee

Park

2002

Biopolymers Online

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Introduction Historical Outline Production of SCL‐PHAs by Bacterial Fermentation Ralstonia eutropha Alcaligenes latus Other Bacteria Biosynthesis and Production of PHAs by Metabolically Engineered Bacteria Recombinant E. coli Recombinant R. eutropha Economic Considerations in the Production of SCL‐PHAs by Bacterial Fermentation Outlook and Perspectives Patents Acknowledgments

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Production of Microbial Polyester by Fermentation of Recombinant Microorganisms

Lee

Choi

2001

Biopolyesters

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Polyhydroxyalkanoates (PHAs) can be produced from renewable sources and are biodegradable with similar material properties and processibility to conventional plastic materials. With recent advances in our understanding of the biochemistry and genetics of PHA biosynthesis and cloning of the PHA biosynthesis genes from a number of different bacteria, many different recombinant bacteria have been developed to improve PHA production for commercial applications. For enhancing PHA synthetic capacity, homologous or heterologous expression of the PHA biosynthetic enzymes has been attempted. Several genes that allow utilization of various substrates were transformed into PHA producers, or non-PHA producers utilizing inexpensive carbon substrate were transformed with the PHA biosynthesis genes. Novel PHAs have been synthesized by introducing a new PHA biosynthesis pathway or a new PHA synthase gene. In this article, recent advances in the production of PHA by recombinant bacteria are described.

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Improvement of hydroxyvalerate fraction in poly(β-hydroxybutyrate-co-β-hydroxyvalerate) by a mutant strain of Alcaligenes eutrophus

Cited by 19 publications

References 7 publications

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) with a broad range of 3HV content at high yields by Burkholderia sacchari IPT 189

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) with a broad range of 3HV content at high yields by Burkholderia sacchari IPT 189

Fermentative Production of SCL Polyhydroxyalkanoates (SCL‐PHAs)

Production of Microbial Polyester by Fermentation of Recombinant Microorganisms

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