Production of Microbial Polyester by Fermentation of Recombinant Microorganisms

Lee, Sang Yup; Choi, Jong-il

doi:10.1007/3-540-40021-4_6

Cited by 25 publications

(20 citation statements)

References 103 publications

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“…After 16-18 h of growth in an orbital shaker (3.5 × g) at 30 or 35 °C (depending on the temperature of the subsequent cultivation, which was the same), an aliquot (calculated OD 600 = 0.1) was utilized as inoculum for the main cultivation in the same synthetic medium, as described below. (Lee and Choi, 2001). All medium components, except the carbon source, and thiamine were sterilized by autoclaving at 121 °C for 15 min.…”

Section: Microorganism and Preservationmentioning

confidence: 99%

Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

Nascimento

Silva

Gomez

et al. 2016

Sci. agric. (Piracicaba, Braz.)

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Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μ max ) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol.

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Section: Microorganism and Preservationmentioning

confidence: 99%

Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

Nascimento

Silva

Gomez

et al. 2016

Sci. agric. (Piracicaba, Braz.)

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“…Decades of biopolyester research were dedicated to understanding the production of these biopolymers and investigating the isolated crystallized biopolymers, their properties and potential applications (Brandl et al 1990, Lee & Choi 2001, Zinn et al 2001. However, the spherical polyester inclusions are now increasingly considered as bioparticles, which can be applied in medicine (Rehm 2006).…”

Section: Introductionmentioning

confidence: 99%

Genetics and Biochemistry of Polyhydroxyalkanoate Granule Self-assembly: The Key Role of Polyester Synthases

Rehm

2006

Biotechnol Lett

117

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PHAs (polyhydroxyalkanoates = biopolyester) composed of hydroxy fatty acids represent a rather complex class of storage polymers synthesized by various bacteria and archaea and are deposited as water-insoluble cytoplasmic nano-sized inclusions. These spherical particles are composed of a polyester core surrounded by phospholipids and proteins. The key enzymes of polyester biosynthesis and polyester particle formation are the polyester synthases, which catalyze the formation of polyesters. Various metabolic routes have been identified and established in bacteria to provide substrate for polyester synthases. Although not essential for particle formation, non-covalently attached proteins, the so-called phasins, can be found at the particle surface and are considered as structural proteins. Protein engineering of polyester synthases and phasins was used to shed light into the topology of these granule attached proteins. Biopolyesters and the respective micro-/nano-structures are currently considered as biocompatible and biodegradable biomaterials with numerous potential applications particularly in the medical field.

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“…This was not only done in Arabidopsis thaliana [Poirier et al, 1992] but also in agriculturally relevant crops being already cultivated on large acreage for production of various food, feed and chemical compounds ( table 2 ). Successful expression of the R. eutropha PHA biosynthesis genes encouraged researchers also to use PHA biosynthesis genes from other microorganisms [Lee and Choi, 2001]. …”

Section: Heterologous Expression Of R Eutropha Pha Biosynthesis Genementioning

confidence: 99%

<i>Ralstonia eutropha</i> Strain H16 as Model Organism for PHA Metabolism and for Biotechnological Production of Technically Interesting Biopolymers

Reinecke¹,

2008

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The Gram-negative, facultative chemolithoautotrophic bacterium Ralstonia eutropha has been intensively investigated for almost 50 years. Today it is the best studied ‘Knallgas’ bacterium and producer of poly(3-hydroxybutyric acid). This polyester provides the basis for renewable resource-based biodegradable plastic materials and has attracted much biotechnological interest. The polymer is accumulated in large amounts in the cell and can be used for various applications ranging from replacement of fossil resource-based bulk plastics to high-value special purpose polymers. To further enhance productivity and to allow tailormade poly(hydroxyalkanoic acids) (PHA) with different monomer compositions by metabolic engineering, the knowledge of metabolic pathways and of the biochemical properties of the enzymes involved is essential. Furthermore, proteins covering the PHA granule surface, which are referred to as phasins, and fusions of these phasins to other proteins are promising candidates for various protein technologies. The recently published genome sequence of strain H16 allows researchers to take a closer look at the genetic potential of this versatile bacterium. R. eutropha is, however, not limited to PHAs and to PHA-related polymers like poly(mercaptoalkanoic acids) as it can also be employed for production of a range of other interesting polymers including polyamides like cyanophycin.

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Production of Microbial Polyester by Fermentation of Recombinant Microorganisms

Cited by 25 publications

References 103 publications

Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

Genetics and Biochemistry of Polyhydroxyalkanoate Granule Self-assembly: The Key Role of Polyester Synthases

<i>Ralstonia eutropha</i> Strain H16 as Model Organism for PHA Metabolism and for Biotechnological Production of Technically Interesting Biopolymers

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