Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) with a broad range of 3HV content at high yields by Burkholderia sacchari IPT 189

Abstract

The biosynthesis of poly(3-hydroxybutyrateco-3-hydroxyvalerate) from sucrose and propionic acid by Burkholderia sacchari IPT 189 was studied using a twostage bioreactor process. In the first stage, this bacterium was cultivated in a balanced culture medium until sucrose exhaustion. In the second stage, a solution containing sucrose and propionic acid as carbon source was fed to the bioreactor at various sucrose/propionic acid (s/p) ratios at a constant specific flow rate. Copolymers with 3HV content ranging from 40 down to 6.5 (mol%) were obtained with 3HV yield from propionic acid (Y 3HV/prop ) increasing from 1.10 to 1.34 g g -1 . Copolymer productivity of 1 g l -1 h -1 was obtained with polymer biomass content rising up to 60% by increasing a specific flow rate at a constant s/p ratio. Increasing values of 3HV content were obtained by varying the s/p ratios. A simulation of production costs considering Y 3HV/prop obtained in the present work indicated that a reduction of up to 73% can be reached, approximating US$ 1.00 per kg which is closer to the value to produce P3HB from sucrose (US$ 0.75 per kg).Keywords Biodegradable polyester Á Burkholderia sacchari Á P(3HB-co-3HV) Á Propionic acid Á Sucrose Á Y 3HV/prop

Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sacchari using wheat straw hydrolysates and gamma-butyrolactone

“…In a recent study with B. sacchari, Mendonça et al, 2013 confirmed the ability of this strain to incorporate the 3HV, 4HB and 3HHx monomers upon the addition of specific co-substrates along with glucose namely odd-chain fatty acids, 4-hydroxybutyric acid and hexanoic acid, respectively [22]. B. sacchari IPT 189 has also been reported in literature to produce the copolymer P(3HB-co-3HV) when grown in sucrose and propionic acid [23].…”

Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains

“…15 Typical carbon sources for the PHA fermentations are sugars and fatty acids, most of the time in mineral salt media (minimal media). 13,16,17 Aside from refined fructose or fructose-containing sugars in minimal media, complex fructose-containing carbon sources have also been used for fermentations and are discussed below (Table 1). The reported results are typically not as good as is the case with minimal media, but medium costs are significantly lower.…”

High cell density strategy for poly(3-hydroxybutyrate) production by Cupriavidus necator

“…Currently, continuous cultures, fed batch and cyclic or repeated fed batch modes are the main cultures for PHA production at high cell density. For P(3HB) production, fed batch cultivations have been widely used in order to achieve high cell density (Rocha et al, 2008;Shang et al, 2003;Wang and Lee, 1997;Yamane et al, 1996;Kulpreecha et al, 2009), which increases the productivity of the system, leading to low investment and reduced production costs.…”