Improvement of dermal burn healing by combining sodium alginate/chitosan-based films and low level laser therapy

Dantas, Marx D. M.; Cavalcante, Danielle Rodrigues Ribeiro; Araújo, Francisco Emanuel Nascimento; Barretto, Sandra Regina; Aciole, Gilberth T. S.; Pinheiro, António Luiz Barbosa; Ribeiro, Maria Amália Gonzaga; Lima-Verde, I.B.; Melo, Cláudia Moura de; Cardoso, Juliana Cordeiro; Júnior, Ricardo Luiz Cavalcanti Albuquerque

doi:10.1016/j.jphotobiol.2011.06.009

Cited by 100 publications

(66 citation statements)

References 29 publications

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“…There are many different therapeutic actions of lasers on tissues, including increased local microcirculation 33 , reduction of the number of inflammatory cells 34 , inhibition of cyclooxygenase-2 (COX-2) and proinflammatory cytokine synthesis 35,36 , increase in collagen synthesis and stimulation of the proliferation of epithelial cells and fibroblasts 37,38 . P.A.…”

Section: Discussionmentioning

confidence: 99%

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Matos

Soares

Júnior

et al. 2014

Rev. odontol. UNESP

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ResumoIntrodução: Medicamentos endodônticos apresentam componentes tóxicos que provocam algum grau de reação inflamatória. Objetivo: Este estudo avaliou o efeito da laserterapia na resposta inflamatória causada por medicações intracanais, em tecido subcutâneo de ratos, por meio da análise quantitativa de mastócitos. Material e método: Tubos de polietileno contendo as medicações foram implantados no dorso de 60 ratos, distribuídos em seis grupos: HS (pasta de hidróxido de cálcio P.A.); HL (pasta de hidróxido de cálcio P.A. e laserterapia); HPS (pasta de hidróxido de cálcio P.A. com paramonoclorofenol canforado); HPL (pasta de hidróxido de cálcio P.A. com paramonoclorofenol canforado e laserterapia); IS (iodofórmio e soro fisiológico) e IL (iodofórmio, soro fisiológico e laserterapia). Os animais foram eutanasiados oito e quinze dias após a cirurgia, as peças cirúrgicas foram removidas, processadas para inclusão em parafina e os cortes histológicos corados em Azul de Toluidina 0.2%, para quantificação dos mastócitos. A análise de variância (ANOVA) e o teste de Tukey post hoc foram aplicados para determinar diferenças significativas entre os grupos quanto ao número de mastócitos (p<0.05). Resultado: Nos grupos HL, HPL e IL houve uma diminuição de mastócitos em ambos os períodos experimentais em relação aos grupos HS, HPS e IS, porém não se observou diferença estatística significativa entre o grupo HPS e o HPL aos oito dias. Conclusão: A laserterapia foi eficaz em modular a intensidade da resposta inflamatória induzida pelos medicamentos endodônticos a partir da redução significativa na quantidade de mastócitos.Descritores: Endodontia; laserterapia; mastócitos; inflamação; subcutâneo. Abstract Introduction: Endodontic medications contain toxic components that cause varying degrees of inflammation.Objective: This study evaluated the effect of laser therapy on the inflammatory response induced by intracanal medications implanted into the subcutaneous tissue of rats using a quantitative analysis of mast cells. Material and method: Polyethylene tubes containing the medications were implanted in the dorsum of 60 rats divided into six groups, including HS (P.A. calcium hydroxide paste), HL (P.A. calcium hydroxide paste and laser therapy), HPS (P.A. calcium hydroxide paste with camphorated paramonochlorophenol), HPL (P.A. calcium hydroxide paste with camphorated paramonochlorophenol and laser therapy), IS (iodoform with saline) and IL (iodoform with saline and laser therapy). The animals were euthanized eight or fifteen days after surgery, and samples were removed and embedded in paraffin. Histological sections were stained with 0.2% toluidine blue for the quantification of mast cells. Analysis of variance (ANOVA) and Tukey's post-hoc test were applied to determine significant differences in the number of mast cells between groups (p<0.05). Result: There was a decrease in mast cells for the HL, HPL and IL groups when compared with the HS, HPS and IS groups at both time points. There was no statistically significant differe...

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Section: Discussionmentioning

confidence: 99%

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Matos

Soares

Júnior

et al. 2014

Rev. odontol. UNESP

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“…15 It has been also demonstrated that films of this polysaccharide and chitosan in combination with laser therapy help the epithelization and vascularization of dermal burns. 16 Furthermore, since burn wounds are highly exposed to the risk of microbial colonization that possibly determines local and systemic infections and delays the healing process, 17,18 alginate dressings containing silver 19,20 and other antimicrobial compounds 21 have been developed. Despite the benefits offered by Ca-alginate based biomedical devices, studies in literature have pointed out that the release of Ca 2+ ions from the dressing to the wound site stimulates the production of pro-inflammatory cytokines, such as Interleukin-6 (IL-6), Interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and the chemotactic cytokine IL-8.…”

Section: Introductionmentioning

confidence: 99%

Alginate–lavender nanofibers with antibacterial and anti-inflammatory activity to effectively promote burn healing

et al. 2016

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This is an Accepted Manuscript, which has been through the Royal Society of Chemistry peer review process and has been accepted for publication.Accepted Manuscripts are published online shortly after acceptance, before technical editing, formatting and proof reading. Using this free service, authors can make their results available to the community, in citable form, before we publish the edited article. We will replace this Accepted Manuscript with the edited and formatted Advance Article as soon as it is available.You can find more information about Accepted Manuscripts in the Information for Authors. One of the current challenges in wound care is the development of multifunctional dressings that can both protect the wound from external agents and promote the regeneration of the new tissue. Here, we show the combined use of two naturally derived compounds, sodium alginate and lavender essential oil, for the production of bioactive nanofibrous dressings by electrospinning, and their efficacy for the treatment of skin burns induced by midrange ultraviolet radiation (UVB). We demonstrate that the engineered dressings reduce the risk of microbial infection of the burn, since they stop the growth of Staphylococcus aureus. Furthermore, they are able to control and reduce the inflammatory response that is induced in human foreskin fibroblasts by lipopolysaccharides, and in rodents by UVB exposure. In particular, we report a remarkable reduction of pro-inflammatory cytokines when fibroblasts or animals are treated with the alginate-based nanofibers. The down-regulation of cytokines production and the absence of erythema on the skin of the treated animals confirm that the here described dressings are promising as advanced biomedical devices for burn management.

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“…Moreover, its porous nanofiber structure was excellent at avoiding dehydration in the area and simultaneously maintained the cellular microenvironment, effectively protecting against bacterial infections. 13,16,23,24 At the experimental time of 21 days, all of the therapeutic protocols used in this study (LED, LED + D, and US + D) presented statistically significant results with increased formation of collagen fibers and an advanced stage of remodeling of these fibers, with a predominance of thick and organized fibers (demonstrating their maturity) when compared with the control. The remodeling of collagen, by the thickening of the fibers, is probably due to the replacement of type III collagen by type I.…”

Section: Discussionmentioning

confidence: 76%

“…The association of the dressing determined the modulation of reepithelialization, neoangiogenesis, collagen formation and also promoted the rapid replacement of collagen type III fibers by type I fibers and the remodeling of these fibers in the injured area. 24 Currently there is not much information about the biomolecular alterations that occur during the evolution of the repair process when applied with different therapies. Therefore, this study aimed to compare two technical analyses, histomorphometry (the gold standard) and FT-IR (noninvasive), to evaluate the molecular alterations and the histological characteristics observed with the association of LED or ultrasound with a semipermeable dressing in the healing process of surgical wounds in rats.…”

Section: Clinical Problem Addressedmentioning

confidence: 99%

Comparative Study of Morphometric and Fourier Transform Infrared Spectroscopy Analyses of the Collagen Fibers in the Repair Process of Cutaneous Lesions

Cronemberger

Raniero

Bueno

et al. 2016

Advances in Wound Care

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Objective: Compare the efficacy of light-emitting diode (LED) and therapeutic ultrasound (TUS), combined with a semipermeable dressing (D), at forming collagen in skin lesions by morphometry and Fourier transform infrared spectroscopy (FT-IR). Materials and Methods: Surgical skin wounds (2.5 cm) were created on 84 male Wistar rats divided into four groups (n = 21): Group I (Control), Group II (LED), Group III (LED + D), and Group IV (US + D). On days 7, 14, and 21, the tissue samples were removed and divided into two pieces, one was used for histological examination (collagen) and the other for FT-IR. Results: The histomorphometric analysis showed no significant differences among groups for collagen deposition at 7 days. However, at 14 days, more deposition of collagen was noted in the groups LED ( p < 0.05) and LED + D ( p < 0.001) than in the control. At 21 days, the groups LED, LED + D, and US + D presented significantly greater deposition of collagen when compared with the control. The FT-IR spectra, at 14 days, LED + D had greater amounts of type I collagen, a better organization of fibers, and greater difference of mean separation between the groups, not observed at 7 and 21 days. Innovation: The histomorphometric and FT-IR analyses suggest that the association of semipermeable dressing to LED therapy and to TUS modulates biological events, increasing fibroblast/collagen response and accelerating dermal maturation. Conclusion: The histomorphometric and FT-IR analyses showed that LED therapy is more efficacious than TUS, when combined with a semipermeable dressing, and induced the collagen production in skin lesions.

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Improvement of dermal burn healing by combining sodium alginate/chitosan-based films and low level laser therapy

Cited by 100 publications

References 29 publications

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Alginate–lavender nanofibers with antibacterial and anti-inflammatory activity to effectively promote burn healing

Comparative Study of Morphometric and Fourier Transform Infrared Spectroscopy Analyses of the Collagen Fibers in the Repair Process of Cutaneous Lesions

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