Comparative Study of Morphometric and Fourier Transform Infrared Spectroscopy Analyses of the Collagen Fibers in the Repair Process of Cutaneous Lesions

Abstract

Objective: Compare the efficacy of light-emitting diode (LED) and therapeutic ultrasound (TUS), combined with a semipermeable dressing (D), at forming collagen in skin lesions by morphometry and Fourier transform infrared spectroscopy (FT-IR). Materials and Methods: Surgical skin wounds (2.5 cm) were created on 84 male Wistar rats divided into four groups (n = 21): Group I (Control), Group II (LED), Group III (LED + D), and Group IV (US + D). On days 7, 14, and 21, the tissue samples were removed and divided into two pieces, one was used for histological examination (collagen) and the other for FT-IR. Results: The histomorphometric analysis showed no significant differences among groups for collagen deposition at 7 days. However, at 14 days, more deposition of collagen was noted in the groups LED ( p < 0.05) and LED + D ( p < 0.001) than in the control. At 21 days, the groups LED, LED + D, and US + D presented significantly greater deposition of collagen when compared with the control. The FT-IR spectra, at 14 days, LED + D had greater amounts of type I collagen, a better organization of fibers, and greater difference of mean separation between the groups, not observed at 7 and 21 days. Innovation: The histomorphometric and FT-IR analyses suggest that the association of semipermeable dressing to LED therapy and to TUS modulates biological events, increasing fibroblast/collagen response and accelerating dermal maturation. Conclusion: The histomorphometric and FT-IR analyses showed that LED therapy is more efficacious than TUS, when combined with a semipermeable dressing, and induced the collagen production in skin lesions.

Investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy

“…Within the amide III region of the prevention model (Fig. 4 a, ii), the saline group exhibited a peak at 1,335 cm −1 , representing α-helical structures typical of type I collagen 38 , 39 . The OVA and RV group, on the other hand, showed peaks at 1,317, and 1,311 cm −1 , respectively, indicative of β-sheets formations 38 .…”

A Systematic Review on Classification, Identification, and Healing Process of Burn Wound Healing

“…6 In another study, Nogueira et al used FTIR for analyses of the collagen fibers in the repair process of cutaneous lesions. 83 In another study, the structural characteristics of collagen networks, such as the degree of fiber organization, and the secondary structure of collagen (α-helices) were obtained by FTIR and then used to monitor the collagen…”