Improvement of Analytical Methods for the Determination of Polyphenolic Bioactive Compounds in Berry Fruits

Abstract

Berry fruits contain high levels of different phytochemicals, most of which are phenolic molecules. Fruits of the same cultivar from different locations and different harvest years have different chemical compositions, particularly related to polyphenols. The difference may be due to specific climatic conditions, the type of soil in which the plants grow, and the stresses to which the plants were subjected because these phytochemicals are produced as a defense mechanism through a secondary metabolic process. For this reason, it is important to establish simple and reliable procedure to determine polyphenolic compounds in berry fruits considering the increasing attention on these compounds for different potential uses. In order to choose and to improve the most adequate analytical procedure for the determination of the polyphenolic substances in berry fruits, different methods were applied and compared on samples of elderberry and blackberry.

Anti‐atopic dermatitis effects of hydrolyzed celery extract in mice

“…In our previous study, we demonstrated that acid hydrolysis of CE increased the concentration of apigetrin, apigenin, and luteolin, and led to the detection of kaempferol which was not initially detected in the CE (Shin et al, 2019). The results of the study were similar with other studies that suggested that hydrolysis could be used to improve the concentrations of bio‐actives in plant extracts (Caruso et al, 2015; Herrera, Navarro Del Hierro, Fornari, Reglero, & Martin, 2019; Kulshreshtha et al, 2015). As a result, we used acid hydrolyzed and ethanol CEs to study the effects of CE in the amelioration of AD signs and symptoms.…”

Shelf-life evaluation and nutraceutical properties of chia seeds from a recent long-day flowering genotype cultivated in Mediterranean area

“…With the attempt to determine flavonol aglycones and free hydroxycinnamic acids, crude extracts were subjected to acid hydrolysis before injection according the procedure to Caruso et al (2015). Flavonols were determined as aglycons, in particular myricetin, quercetin and kaempferol on the hydrolysed extract by HPLC using a Varian Chromatography System comprising a 9012 pump and a 9050 UV-Vis detector (Varian-Agilent, Milan, Italy) at 360 nm.…”

Chemistry and Functionality of Bioactive Compounds Present in Persimmon

“…Recently, natural bioactive compounds have been acknowledged with greater consideration [3]. Fruits and vegetables are vital parts of the diet and a rich source of bioactive compounds including dietary fiber, natural antioxidants, and various phytochemicals [3,4,7]. The individual phytochemicals that have been recognized are about 5000 in number, but large numbers of them are still unknown or unidentified [8].…”