Improved Separation and Determination of Phospholipids in Animal Tissues Employing Solid Phase Extraction.

Upregulation of a desaturase is associated with the enhancement of cold hardiness in the onion maggot, Delia antiqua

“…The isolation of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) was performed by the method of Suzuki et al (1997). The total lipids dissolved in 1 ml of a mixture of hexane, chloroform and methanol (95:3:2 v/v/v) were applied to an NH 2 -phase cartridge (VARIAN), and washed with 3 ml of a mixture of petroleum ether, diethyl ether and acetic acid (80:20:3 v/v/v) to remove fatty acid ester, wax and cholesterol ester.…”

Effect of linseed oil supplementation on concentrations of (n‐3) polyunsaturated fatty acids in liver phospholipids of rats fed diets containing either an oil rich in conjugated linoleic acids, sunflower oil or high‐oleic acid sunflower oil

“…Total lipids of the liver were extracted with a mixture of hexane and isopropanol (3 : 2 v/v) (Hara and Radin, 1978). Phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE) were separated by a solid phase extraction method described by Suzuki et al (1997) with modifications. Briefly, aliquots of the total lipids extracts were dried under nitrogen.…”

Selective Enrichment of Phospholipids by Titania

“…The second step is the fractionation of phospholipids from the total lipids, and it is carried out mainly by acetone precipitation, 4 scraping from a developed thin-layer plate and solid-phase extraction. 5,6 We previously reported on a high-performance liquid chromatography (HPLC) system with postcolumn fluorescence detection for the determination of phospholipids, based on a series of hydrolysis under UV-irradiation in the presence of TiO2, phosphomolybdic acid and thiamine-thiochrome reactions. 7 However, when the actual samples were repeatedly analyzed by the system, the column life time seemed to be relatively short, because of the accumulation of proteinous substances in the samples.…”