Selective Enrichment of Phospholipids by Titania

Ikeguchi, Yoshihiko; Nakamura, Hiroshi

doi:10.2116/analsci.16.541

Cited by 75 publications

(49 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For some years, TiO 2 was used in bioelectrochemical studies of protein functions using the adsorption of proteins to TiO 2 films [63]. More interestingly for phosphoproteomic studies, TiO 2 was shown to have affinity for phosphate ions from aqueous solutions [64][65][66][67][68][69][70][71]. In 2004, Pinkse and co-workers presented an online 2-D LC strategy for phosphopeptide analysis with spherical particles of titanium dioxide (Titansphere) as the first dimension and RP material as the second dimension.…”

Section: Titanium Dioxide Chromatographymentioning

confidence: 99%

Analytical strategies for phosphoproteomics

2009

View full text Add to dashboard Cite

Protein phosphorylation is a key regulator of cellular signaling pathways. It is involved in most cellular events in which the complex interplay between protein kinases and protein phosphatases strictly controls biological processes such as proliferation, differentiation, and apoptosis. Defective or altered signaling pathways often result in abnormalities leading to various diseases, emphasizing the importance of understanding protein phosphorylation. Phosphorylation is a transient modification, and phosphoproteins are often very low abundant. Consequently, phosphoproteome analysis requires highly sensitive and specific strategies. Today, most phosphoproteomic studies are conducted by mass spectrometric strategies in combination with phosphospecific enrichment methods. This review presents an overview of different analytical strategies for the characterization of phosphoproteins. Emphasis will be on the affinity methods utilized specifically for phosphoprotein and phosphopeptide enrichment prior to MS analysis, and on recent applications of these methods in cell biological applications.

show abstract

Section: Titanium Dioxide Chromatographymentioning

confidence: 99%

Analytical strategies for phosphoproteomics

2009

View full text Add to dashboard Cite

show abstract

“…21 Therefore, the first step in the optimization of the spin column extraction procedure was evaluated; 0.2 ml aliquots of serum and urine samples were added to acetic acid (10, 25, 50, and 100 μl) and 0.2 ml water. This sample was applied to the conditioned monolith spin column.…”

Section: Extractionmentioning

confidence: 99%

“…It has been found that the recoveries of phosphatidyl-ethanol-amine and L-α-phosphatidylcholine dipalmitoyl in egg yolk by titania extraction were 62.8 and 63.1%, respectively. 21 It is thus postulated that phospholipids may adhere to titania more readily than GLUF and GLYP. Moreover, all of the organic phosphates were adsorbed, while sugars, carboxylic acids and other compounds were not much adsorbed on titania.…”

Section: Matrix Effects and Recoverymentioning

confidence: 99%

Mix-mode TiO-C18 Monolith Spin Column Extraction and GC-MS for the Simultaneous Assay of Organophosphorus Compounds and Glufosinate, and Glyphosate in Human Serum and Urine

et al. 2011

View full text Add to dashboard Cite

“…25 Ti(IV) may bind to phosphorylated intracellular proteins, which often correspond to active functional states of enzymes or signaling proteins and interfere with signaling pathways. Additionally, it is reasonable to assume that the attachment of Ti(IV) to membrane phospholipids may change its fluidity and thereby alter cellular functions such as migration, protein secretion, and the ability to respond to stimuli as shown with other metals such as aluminum and nickel.…”

Section: Titanium Uptake In Human T-lymphocytesmentioning

confidence: 99%

Titanium uptake, induction of RANK‐L expression, and enhanced proliferation of human T‐lymphocytes

Cadosch

Sutanto

Chan

et al. 2009

Journal Orthopaedic Research

View full text Add to dashboard Cite

There is increasing evidence that titanium ions are released from orthopedic implants by biocorrosion. The aim of this study was to investigate titanium uptake by human T-lymphocytes and its effects on phenotype and proliferation. Freshly isolated human nonadherent peripheral blood mononuclear cells (NA-PBMC), were exposed to TiCl 4 [Ti(IV)]. Bioavailability and distribution of Ti(IV) in T-lymphocytes was determined by energy-filtered electron microscopy (EFTEM). The effects of Ti(IV) challenge on nonactivated and PHA-activated cells were assessed by flow cytometric analysis of surface markers, RANK-L production, and proliferation assays. EFTEM colocalized Ti(IV) with phosphorus in the nucleus, ribosomes, cytoplasmic membranes, and the surface membrane of T-lymphocytes. Ti(IV) increased significantly the expression of CD69, CCR4, and RANK-L in a concentration-dependent manner. Titanium enters T-lymphocytes through a currently unknown mechanism and binds to phosphorus-rich cell structures. Titanium influences phenotype and function of T-lymphocytes, resulting in activation of a CD69þ and CCR4þ T-lymphocyte population and secretion of RANK-L. These results strongly suggest the involvement of titanium ions challenged T-lymphocytes in the complex pathophysiological mechanisms of aseptic loosening of orthopedic implants. ß

show abstract

Selective Enrichment of Phospholipids by Titania

Cited by 75 publications

References 14 publications

Analytical strategies for phosphoproteomics

Analytical strategies for phosphoproteomics

Mix-mode TiO-C18 Monolith Spin Column Extraction and GC-MS for the Simultaneous Assay of Organophosphorus Compounds and Glufosinate, and Glyphosate in Human Serum and Urine

Titanium uptake, induction of RANK‐L expression, and enhanced proliferation of human T‐lymphocytes

Contact Info

Product

Resources

About