Improved Procedure for Production of Cytochalasin E and Tremorgenic Mycotoxins by
            <i>Aspergillus clavatus</i>

Demain, Arnold L.; Hunt, N. A.; Malik, Vinod; Kobbe, B; Hawkins, H.; Matsuo, Keizo; Wogan, G. N.

doi:10.1128/aem.31.1.138-140.1976

Cited by 32 publications

(14 citation statements)

References 8 publications

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“…The agitated solid substrate fermentation technique has been successfully used for the production of several mycotoxins (1). In the present study it was possible to increase the production of the crude toxin of A. flavus by almost 10-fold as compared with the more conventional static rice technique.…”

mentioning

confidence: 73%

Production of cyclopiazonic acid by Aspergillus flavus Link

Luk¹,

Kobbe²,

Townsend³

1977

Appl Environ Microbiol

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mentioning

confidence: 73%

Production of cyclopiazonic acid by Aspergillus flavus Link

Luk¹,

Kobbe²,

Townsend³

1977

Appl Environ Microbiol

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“…A. clavatus produces patulin, clavatol, c-sarcin, cytochalasin E and tremorgenic mycotoxins [7,25,26]. These reports indicate that Paecilomyces sp.…”

Section: Discussionmentioning

confidence: 85%

Brefeldin A, a cytotoxin produced by Paecilomyces sp. and Aspergillus clavatus isolated from Taxus mairei and Torreya grandis

Wang

2002

FEMS Immunology and Medical Microbiology

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Paecilomyces sp. and Aspergillus clavatus, which were isolated from Taxus mairei and Torreya grandis from southeast China, produced toxic metabolites when grown in liquid culture. Nuclear magnetic resonance techniques, infrared spectrometry, electrospray ionization mass spectroscopy and X-ray analysis identified brefeldin A, a bioactive metabolite produced by a number of fungal species belonging to the genera Alternaria, Ascochyta, Penicillium, Curvularia, Cercospora and Phyllosticta. This is the first report of the isolation of the cytotoxin from Paecilomyces sp. and A. clavatus. The relevance of brefeldin A to the association between these fungi and their host plants is discussed.

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“…Cytochalasins are a group of fungal secondary metabolites with a 10-phenylperhydroisoindol-1-one skeleton and a macrocyclic ring and are capable of various biological activities (Qiao et al 2011). They have been described not only in the genus Aspergillus ( Demain et al 1976 , Udagawa et al 2000 , Lin et al 2009 , Zheng et al 2013) , but also in Xylaria ( Silva et al 2010) , Cladosporium ( Cafêu et al. 2005) , Arthrinium (Wang 2015), and Phomopsis (Shen et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Antifungal activity of extracts from Atacama Desert fungi againstParacoccidioides brasiliensis and identification ofAspergillus felis as a promising source of natural bioactive compounds

Mendes

Gonçalves

Souza-Fagundes

et al. 2016

Mem. Inst. Oswaldo Cruz

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Fungi of the genus Paracoccidioides are responsible for paracoccidioidomycosis. The occurrence of drug toxicity and relapse in this disease justify the development of new antifungal agents. Compounds extracted from fungal extract have showing antifungal activity. Extracts of 78 fungi isolated from rocks of the Atacama Desert were tested in a microdilution assay against Paracoccidioides brasiliensis Pb18. Approximately 18% (5) of the extracts showed minimum inhibitory concentration (MIC) values≤ 125.0 µg/mL. Among these, extract from the fungus UFMGCB 8030 demonstrated the best results, with an MIC of 15.6 µg/mL. This isolate was identified as Aspergillus felis (by macro and micromorphologies, and internal transcribed spacer, β-tubulin, and ribosomal polymerase II gene analyses) and was grown in five different culture media and extracted with various solvents to optimise its antifungal activity. Potato dextrose agar culture and dichloromethane extraction resulted in an MIC of 1.9 µg/mL against P. brasiliensis and did not show cytotoxicity at the concentrations tested in normal mammalian cell (Vero). This extract was subjected to bioassay-guided fractionation using analytical C18RP-high-performance liquid chromatography (HPLC) and an antifungal assay using P. brasiliensis. Analysis of the active fractions by HPLC-high resolution mass spectrometry allowed us to identify the antifungal agents present in the A. felis extracts cytochalasins. These results reveal the potential of A. felis as a producer of bioactive compounds with antifungal activity.

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Improved Procedure for Production of Cytochalasin E and Tremorgenic Mycotoxins by Aspergillus clavatus

Abstract: This report describes an improved procedure for production of cytochalasin E and tremorgens by solid substrate, agitated fermentation ofAspergillus clavatus on pearled barley.

Cited by 32 publications

References 8 publications

Production of cyclopiazonic acid by Aspergillus flavus Link

Production of cyclopiazonic acid by Aspergillus flavus Link

Brefeldin A, a cytotoxin produced by Paecilomyces sp. and Aspergillus clavatus isolated from Taxus mairei and Torreya grandis

Antifungal activity of extracts from Atacama Desert fungi againstParacoccidioides brasiliensis and identification ofAspergillus felis as a promising source of natural bioactive compounds

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