The improvement of superior wheat cultivars depends on the identification of promising segregating populations to derive superior lines. A lattice model (8×8) involving 56 F 2 populations and eight parents was conducted in the 2020 cropping season, and grain yield per plant was evaluated for every F 2 population, with further analysis of the population potential by Jinks and Pooni method via REML/BLUP. A total of 5,410 F 2 plants were evaluated in this study. The results showed that the use of genetic variance associated with the individual genotypic value (BLUP) was superior compared with the use of variance and traditional phenotypic values.