Improved distribution analysis of cobalamins and cobalamin analogues in human plasma in which the use of thiol-blocking agents is a prerequisite

Kapel, J. Van; Spijkers, L. J. M.; Lindemans, Jan; Abels, J.

doi:10.1016/0009-8981(83)90090-6

Cited by 19 publications

(11 citation statements)

References 29 publications

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“…Elle est formée de plusieurs formes chimiques (cobalamines ou isomères de la vitamine 8 12 ) dont les principales sont la cyanocobalamine (CNCbl), l'hydroxocobalamine (OHCbl), la méthylcobalamine (MeCbl) et l'adénosylcobalamine (AdoCbl). Ces cobalamines sont généralement liées aux protéines dans les aliments et les tissus (Kapel et al, 1983).…”

Section: Introductionunclassified

“…Mais, compte tenu de la faible quantité de certaines cobalamines dans les tissus et le plasma, Frenkel et al (1980), de même que Kapel et al (1983), ont combiné le système de gradient de HPLC avec la méthode de dilution isotopique pour mesurer les cobalamines respectivement dans les tissus et dans le plasma. Cette méthode de radiodilution repose sur le principe d'une inhibition compétitive de la vitamine 8 12 non marquée et de la vitamine 8 12 radiomarquée dans une solution titrée de facteur intrinsèque.…”

Section: Introductionunclassified

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Effet des traitements thermiques sur la mise en disponibilité de la vitamine B₁₂dans le lait

Fie

Zee

Amiot

1994

Lait

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(Reçu le 22 juillet 1993 ; accepté le 7 juillet 1994) Résumé -La combinaison d'une méthode de séparation par HPLC et d'une méthode de dosage par radio-essai a permis de montrer que les quantités d'adénosylcobalamine et de méthylcobalamine diminuent dans les laits pasteurisés, stérilisés et séchés, tandis que celle de la cyanocobalamine augmente dans ces trois laits. Quant à l'hydroxocobalamine, sa quantité est diminuée dans le lait pasteurisé ainsi que dans le lait stérilisé mais augmente dans le lait séché par rapport au lait cru. Une méthode in vitro de digestion gastrique et intestinale, mettant en jeu la pepsine, la pancréatine et les facteurs "R" et intrinsèque, combinée aux 2 premières méthodes de dosage, a permis d'évaluer la mise en disponibilité des cobalamines dans du lait soumis à différents traitements thermiques.Ainsi, la vitamine 8 12 serait plus disponible dans le lait pasteurisé (1,91 pmol/ml) que dans le lait cru (1,54 pmol/ml), le lait stérilisé (1,25 pmol/ml) et le lait en poudre (1,27 pmol/ml), indiquant une influence possible des traitements thermiques sur la biodisponibilité de la vitamine 812 dans ces produits, ainsi que des interconversions des isomères de la vitamine 8 12 au cours du processus de la digestion in vitro.

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Section: Introductionunclassified

Effet des traitements thermiques sur la mise en disponibilité de la vitamine B₁₂dans le lait

Fie

Zee

Amiot

1994

Lait

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“…Djalali et al (1990) measured corrinoids in human plasma with HPLC/ RIDA and found similar amounts of ado-cbl and me-cbl (39 and 33%, respectively) and only small amounts of analogues (9%) in plasma. Other researchers (van Kapel et al, 1983;Matthews, 1979;Nexø et al, 1982) found a much higher percentage of me-cbl (47-65%), measured with HPLC/RIDA and TLC/bioautography, respectively (Table 6). Van Kapel et al (1983) Analysis of corrinoids in ovine tissues 813 ORIGINAL RESEARCH found a much larger proportion of analogues (20-30%) than Djalali et al (1990), an amount similar to the results of this research (18%).…”

Section: Corrinoids In Other Biological Samplesmentioning

confidence: 94%

“…Other researchers (van Kapel et al, 1983;Matthews, 1979;Nexø et al, 1982) found a much higher percentage of me-cbl (47-65%), measured with HPLC/RIDA and TLC/bioautography, respectively (Table 6). Van Kapel et al (1983) Analysis of corrinoids in ovine tissues 813 ORIGINAL RESEARCH found a much larger proportion of analogues (20-30%) than Djalali et al (1990), an amount similar to the results of this research (18%). The variation between authors and methods is large and therefore perhaps only semi-quantitative statements can be made.…”

Section: Corrinoids In Other Biological Samplesmentioning

confidence: 94%

Analysis of corrinoids in ovine tissues

Kelly¹,

Gruner

Furlong

et al. 2006

Biomed. Chromatogr.

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Corrinoids from various ovine tissue samples (liver, blood, small intestinal fluid and faeces) were analysed using a combination of high-performance liquid chromatography (HPLC) and a radioisotope dilution assay (RIDA) to estimate the distribution of corrinoids--the cobalamins hydroxocobalamin (OH-cbl), methylcobalamin (me-cbl) and 5'-deoxyadenosylcobalamin (ado-cbl), and cobalamin analogues--in these tissues. Samples were taken from either cobalt-deficient or cobalt-replete ewes, and ruminant and pre-ruminant lambs. In liver, ado-cbl predominated, followed by analogues, OH-cbl and me-cbl. Supplementation with either cobalt (ruminant) or vitamin B12 injections (pre-ruminant) increased the amount of ado-cbl and decreased analogues. In blood, OH-cbl predominated, followed by ado-cbl, analogues and me-cbl, respectively. In small intestinal fluid, the distribution from largest to smallest percentage was analogues, ado-cbl, OH-cbl and me-cbl. In faeces, analogues constituted the greatest proportion, followed by OH-cbl, ado-cbl and me-cbl, respectively. Owing to the small sample sizes only cautionary interpretations can be made. In contrast to humans, where me-cbl constitutes the highest proportion of corrinoids in plasma and ado-cbl in the liver, in sheep the amount of ado-cbl was consistently higher than me-cbl in all tissues. This may be due to the higher metabolic need of sheep for ado-cbl due to gluconeogenesis. Analogues and OH-cbl were found in each tissue, contrary to previous postulations. The much higher amount of vitamin B12 in small intestinal fluid compared with faeces indicates that a large proportion of the vitamin is absorbed by the gastro-intestinal tract.

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“…Vitamin B12 content in some species of seaweed. B12 contents were assayed microbiologically using E. coli 215 (7) on the extract of seaweeds prepared as described by van Kapel et al (6). treatment with IF and disappeared after treatment with HC corresponds to that of cobinamide, a B12 analogue.…”

Section: Characterization Of B12mentioning

confidence: 99%

Content and Characteristics of Vitamin B12 in Some Seaweeds.

Yamada

Shibata

Takayama

et al. 1996

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThe vitamin B12 (B12) content in seven species of seaweed that are consumed frequently in Hokkaido, Japan, was microbiologically measured using Escherichia coil 215. Asakusanori (Porphyra tenera), maruba-amanori (Porphyra suborbiculata) and akaba-gin-nansou (Rhodo giossum pulcherum) showed higher B12 content than the other species, although the content varied greatly among samples in the same species. A bioautography on a thin-layer plate holding a mixture of silica gel and cellulose, differentiation of B12 and its analogues using a binding specificity of intrinsic factor and haptocorrin, and comparison of the B12 concentra tion determined by the radioisotope dilution assay method using the intrinsic factor as the B12-binding protein with that by the bioassay method, predominantly showed B12 in maruba-amanori and B12 analogues in wakame (Undaria pinnatifida) and akaba-gin-nansou. The B12 uptake of akaba-gin-nansou from artificial seawater was similar to that of asaku sanori that contained only B12. Key Words vitamin B12, seaweed, B12 analogue, bioassay, radioassay, B12 uptakeThe occurrence of vitamin B12 (B12) was believed confined to the animal kingdom until the early 1950s when the B12 content was found to be higher in some seaweeds than beef or fish meat (1). Accordingly, seaweeds were expected to be promising resources of B12 (2) because of their vast production in the world. Most seaweeds contain not only B12 analogues that are active in mammals, but also other * To whom correspondence should be addressed . B12 analogues (2). The biological and nutritional significance of B12 and its analogues in seaweeds have not been reported. However, only Dagnelie et al. reported a deleterious effect on mean corpuscular volume in vegetarian children with a tendency of B12 deficiency when fed some seaweeds including noni (Porphyra family) (3). The findings of Dagnelie et al. may be due to B12 analogues in the seaweeds. The purpose of this study was to get some clue as to the significance of B12 analogues in some seaweeds for human nutrition. For this reason, B12 content was assayed microbiologically using Escherichia coli 215. It responds to a wider variety of B12 and B12 analogues than any other test organism (4). We measured the B12 contents in some commercially available seaweeds that were consumed frequently in Japan. The structure of B12 and its analogues in maruba-amanori (Porphyra suborbiculata ), akaba-gin-nansou (Rhodoglossum pulcherum) and wakame (Und aria pinnatifida) were characterized.Asakusanori containing only B12 has been known to take up B12 from artificial seawater (5). The relationship between B12 content and B12 uptake by a seaweed was worth studying. The B12 uptake by akaba-gin-nansou, composing exclusively of B12 analogues, was measured and compared with that of asakusanori.

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Improved distribution analysis of cobalamins and cobalamin analogues in human plasma in which the use of thiol-blocking agents is a prerequisite

Cited by 19 publications

References 29 publications

Effet des traitements thermiques sur la mise en disponibilité de la vitamine B₁₂dans le lait

Effet des traitements thermiques sur la mise en disponibilité de la vitamine B₁₂dans le lait

Analysis of corrinoids in ovine tissues

Content and Characteristics of Vitamin B12 in Some Seaweeds.

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Improved distribution analysis of cobalamins and cobalamin analogues in human plasma in which the use of thiol-blocking agents is a prerequisite

Cited by 19 publications

References 29 publications

Effet des traitements thermiques sur la mise en disponibilité de la vitamine B12 dans le lait

Effet des traitements thermiques sur la mise en disponibilité de la vitamine B12 dans le lait

Analysis of corrinoids in ovine tissues

Content and Characteristics of Vitamin B12 in Some Seaweeds.

Contact Info

Product

Resources

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Effet des traitements thermiques sur la mise en disponibilité de la vitamine B₁₂dans le lait

Effet des traitements thermiques sur la mise en disponibilité de la vitamine B₁₂dans le lait