Improved cryopreservability of stallion sperm using a sorbitol-based freezing extender

Pojprasath, T.; Lohachit, Chainarong; Techakumphu, Mongkol; Stout, T.A.E.; Tharasanit, Theerawat

doi:10.1016/j.theriogenology.2011.01.014

Cited by 24 publications

(26 citation statements)

References 39 publications

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“…However, lactose (disaccharide) is commonly used for the cryopreservation of boar semen by many researchers [8,9,16,17]. In our previous studies [10,17] [24]. The stallion's cryopreserved sperms utilize sorbitol in the semen extender better than fructose and glucose [24].…”

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confidence: 97%

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Cryopreservation of Boar Semen by Egg Yolk-Based Extenders Containing Lactose or Fructose is Better Than Sorbitol

2012

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ABSTRACT. The present study determined the effect of different types of sugars (lactose, fructose, glucose and sorbitol) used in egg yolkbased extender on the post-thawed boar semen quality. Twenty-two ejaculates from 6 fertility-proven Yorkshire boars were cryopreserved by liquid nitrogen vapor method. Sperm motility, viability, acrosome integrity and intact functional plasma membrane were determined at 0, 2 and 4 hr after thawing. It was found that the lactose-based extender resulted in a higher percentage of post-thawed sperm motility, viability, intact acrosome and functional plasma membrane than sorbitol-based extender (P<0.05) and fructose-based extender yielded a higher post-thawed sperm motility and viability than sorbitol-based extender (P<0.05). It could be concluded that sorbitol was not an effective sugar for the cryopreservation in boar semen. Boar semen cryopreservation greatly facilitates the distribution of valuable and desirable genes because the frozen semen can be transported over a long distance; and a stock of frozen semen enables the use of boar that is dead or ill [3]. However, the use of frozen-thawed (FT) boar semen is limited due to the low fertility outcomes compared to the extended fresh semen [9]. During cryopreservation, both physical and chemical factors including the rapid change in temperature or thermal stress, oxidative stress and osmotic stress lead to the damage of the sperm plasma membrane [19]. In general, the egg yolk-based freezing extender of boar semen consists of cryoprotectant, sugar, buffer and antibiotic [16]. Glycerol is the most common cryoprotectant used in the egg yolk-based semen extender for cryopreservation of the boar semen [14] and egg yolk is regarded as a non-permeable cryoprotectant. Sugar is mainly an energy source of the boar sperm. Furthermore, it protects the boar sperm from dehydration and intracellular ice formation during the semen freezing process [27]. Different types of sugar, e.g., trehalose, lactose, fructose, have been used in the freezing extender of boar semen [15,18].Recently, special interest on researches concerning with the energy utilization of the boar sperm have been noticed [13,15,18,20]. In general, the boar sperm is able to utilize a variety of substrates to obtain energy. Generally, monosaccharides are more common energy source used in extended fresh boar semen than disaccharides [20]. However, lactose (disaccharide) is commonly used for the cryopreservation of boar semen by many researchers [8,9,16,17]. In our previous studies [10,17] [24]. The stallion's cryopreserved sperms utilize sorbitol in the semen extender better than fructose and glucose [24]. In the boar, information associated with the effect of sugar types used in egg yolk-based semen extender on the FT semen qualities is limited. Therefore, the objective of the present study was to determine the effect of different sugar types (lactose, fructose, glucose and sorbitol) used in egg yolk-based semen extender on the sperm motility, sperm viability, acrosome integrity a...

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confidence: 97%

“…In our previous studies [10,17] [24]. The stallion's cryopreserved sperms utilize sorbitol in the semen extender better than fructose and glucose [24]. In the boar, information associated with the effect of sugar types used in egg yolk-based semen extender on the FT semen qualities is limited.…”

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confidence: 99%

Cryopreservation of Boar Semen by Egg Yolk-Based Extenders Containing Lactose or Fructose is Better Than Sorbitol

2012

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“…La aplicación de técnicas de asistencia reproductiva tales como inseminación artificial (IA) con semen congelado permiten aumentar el potencial reproductivo de los equinos (Pojprasath et al, 2011). La criopreservación de espermatozoides es de gran relevancia para la industria de reproducción equina, permitiendo el almacenamiento por períodos de tiempo prolongados y el transporte de semen a lugares distantes (Hoffmann et al, 2011) y utilizar a los potros simultáneamente en reproducción y en competición (Schober et al, 2007).…”

Section: Introductionunclassified

Selección Espermática en Semen Congelado/Descongelado de Equino: Evaluación de las Membranas Plasmática, Acrosomal y Potencial de Membrana Mitocondrial

2014

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RESUMEN:Los procedimientos de criopreservación inducen cambios morfofuncionales en los espermatozoides. Es importante post descongelación espermática utilizar procedimientos de selección que permitan recuperar espermatozoides altamente funcionales. El objetivo del presente estudio fue comparar la eficiencia del Swim-up y Equipure® en la selección de espermatozoides funcionales en semen descongelado de equino. Semen de 4 potros reproductores Criollos Chilenos (A, B, C y D), fueron descongelados separadamente y procesados (n=15) por: I.-Swim-up (SU) y II.-Equipure® (EQ). Post descongelación se determinó por citometría de flujo la viabilidad e integridad de membrana plasmática (SYBR-14/PI), potencial de membrana mitocondrial (Ψ∆m; JC-1), integridad de la membrana acrosomal (FITC-PSA/PI). La motilidad progresiva (%) en dos animales fue más alta (P<0,05) por SU comparado con EQ: A (55,7±5,8% v/s 38,17±3,7%) y C (37,5±7% vs. 32±2,1%, respectivamente). La integridad de la membrana plasmática (%), tres animales presentaron diferencias (P<0,05), siendo más alta por SU en dos animales comparado con EQ (A: 54,3±1,7 vs. 36,7±1,9, C: 36,1±5,7 vs. 29,4±4,8 y D: 34,4±9,4 vs. 52,7±5,2; respectivamente), solamente un animal fue superior EQ. En el Ψ∆m (%), diferencias significativas (P<0,05) fueron detectadas en los cuatro animales, siendo más altos en SU comparado con EQ (A: 69,1±8,6% vs. 47,4±3,3%, B: 59,34±12,3% vs. 24,8±1,5%, C: 54,9±12,3% vs. 43,2±3,1% y D: 53,1±17,6% vs. 37,5±5,7%; respectivamente). Los resultados obtenidos en el presente estudio demostraron que los métodos de selección espermática Swim-up y Equipure® permiten recuperar espermatozoides de diferente calidad funcional en semen congelado-descongelado de equino, presentándose diferencias individuales entre los animales con respecto a los métodos. Se observó una tendencia del método Swim-up en seleccionar espermatozoides de equino descongelados con mayor calidad funcional comparado con Equipure®. PALABRAS CLAVE. Equino; Espermatozoide; Swim-up; Equipure® INTRODUCCIÓNLa aplicación de técnicas de asistencia reproductiva tales como inseminación artificial (IA) con semen congelado permiten aumentar el potencial reproductivo de los equinos (Pojprasath et al., 2011). La criopreservación de espermatozoides es de gran relevancia para la industria de reproducción equina, permitiendo el almacenamiento por períodos de tiempo prolongados y el transporte de semen a lugares distantes (Hoffmann et al., 2011) y utilizar a los potros simultáneamente en reproducción y en competición (Schober et al., 2007). A pesar de la creciente demanda comercial para utilizar semen congelado de equino, la tasa de preñez después de la IA es más baja comparadas con las obtenidas con semen fresco o refrigerado, e incluso inferiores a la obtenida en otras especies domésticas (López-Fernández et al., 2007). Es importante señalar que, existe un alto grado de variabilidad en la calidad del semen entre los machos e incluso entre los eyaculados de un mismo animal (Dowsett & Knott, 1996) como también en ...

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“…Sugars serve as the nutrient for sperm. The most commonly used sugar to preserve and cryo-preserve equine semen is glucose (Bozkurt et al, 2007;Pojprasath et al, 2011). Fructose is easier than glucose to be metabolized by sperm, and is commonly included in chilled and frozen semen extenders in different species (Aboagla & Terada, 2004a;Silva Silva et al, 2005;Akhter el al., 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Fructose is easier than glucose to be metabolized by sperm, and is commonly included in chilled and frozen semen extenders in different species (Aboagla & Terada, 2004a;Silva Silva et al, 2005;Akhter el al., 2010). Trehalose and raffinose are extracellular cryoprotectants which stabilize plasma membrane; both of these are commonly supplemented in frozen semen extenders (Hu et al, 2010;Pojprasath et al, 2011;Slanina et al, 2012). Sperm plasma membrane damage not only occurs during freezing, but also during cooling (Aurich, 2005) refers chilling injury.…”

Section: Introductionmentioning

confidence: 99%

The Quality of Stallion Semen in Skim Milk and Dimitropoulos Extenders Preserved at 5 oC and Ambient Temperature Supplemented with Different Sugar

Arifiantini¹,

Purwantara²,

Yusuf³

et al. 2013

med pet

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This study was conducted to evaluate the effects of sugars supplementation in skim milk based (SM) and dimitropoulos (DV) extenders on the sperm motility and viability in stallion semen storage at 5 o C and ambient temperature (24-29 o C). Semen samples were collected from 3 stallions; evaluate individually and each of them divided into 8 aliquots. Four out of eight aliquots were diluted 1:1 with SM, while the remaining four were diluted 1:1 with DV; all were then centrifuged at 1006 g (3000 RPM) for 15 min. The supernatants were discarded, and each pellet was re-diluted with SM (control), SM trehalose (SMT), SM-raffinose (SMR), SM-fructose (SMF), DV (control), DV-trehalose (DVT), DV-raffinose (DVR), and DV-fructose (DVF). The diluted semen were divided into 2 aliquots and stored at 5 o C or ambient temperature. The sperm motility and viability were evaluated every 3 h on chilled semen stored at ambient temperature, and every 12 h on those stored at 5 o C. Results of the experiments demonstrated that sperm motility and viability in DV extender significantly higher (P<0.05) in both temperature. The supplementation of fructose was the best on the motility and viability of the sperm at both temperatures compare to trehalose, raffinose, or the control group. The best extender and sugar combination was DVF, which the total motile sperm stored at 5 o C for 96 h was 45.1% followed by to DVT (40.2%) and DVR (39.2%). The sperm motility in DV and SMF were 35.3% and 35.6%, respectively; these were higher than those diluted with control (28.9%); SMT (30.3%), and SMR (29.6%). The study concluded that the supplementation of fructose in DV extender (DVF) was the best combination to preserve stallion sperm motility and viability stored at 5 o C or ambient temperature.

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Improved cryopreservability of stallion sperm using a sorbitol-based freezing extender

Cited by 24 publications

References 39 publications

Cryopreservation of Boar Semen by Egg Yolk-Based Extenders Containing Lactose or Fructose is Better Than Sorbitol

Cryopreservation of Boar Semen by Egg Yolk-Based Extenders Containing Lactose or Fructose is Better Than Sorbitol

Selección Espermática en Semen Congelado/Descongelado de Equino: Evaluación de las Membranas Plasmática, Acrosomal y Potencial de Membrana Mitocondrial

The Quality of Stallion Semen in Skim Milk and Dimitropoulos Extenders Preserved at 5 oC and Ambient Temperature Supplemented with Different Sugar

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