Selección Espermática en Semen Congelado/Descongelado de Equino: Evaluación de las Membranas Plasmática, Acrosomal y Potencial de Membrana Mitocondrial

Cabrera, Paulina; Sánchez, Raúl; Risopatrón, Jennie

doi:10.4067/s0717-95022014000200057

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…In donkeys, as in other mammalian species, a high inter- and intra-male variability in the ability of sperm to withstand cryopreservation has been reported [ 29 , 30 , 31 , 32 , 33 , 34 ], leading to the classification of males/ejaculates as of “good” (GFE) or “poor” (PFE) freezability [ 17 , 31 , 35 , 36 ]. Differences in the composition of SP surrounding sperm among individuals and ejaculates could explain such differences [ 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance

et al. 2022

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This study investigated whether the activities of the antioxidant components of donkey seminal plasma (SP)—both enzymatic (superoxide dismutase (SOD), catalase-like (CAT), glutathione peroxidase-like (GPX), and paraoxonase type 1 (PON1)) and non-enzymatic (measured in terms of total thiol, copper-reducing antioxidant capacity (CUPRAC), ferric-reducing ability of plasma (FRAP), and Trolox equivalent antioxidant capacity (TEAC))—and oxidative stress index (OSI) are related to sperm cryotolerance. For this purpose, 15 ejaculates from jackasses (one per individual) were collected and split into two aliquots. The first one was used for measuring the activities levels of enzymatic and non-enzymatic antioxidants and OSI in SP, whereas the other aliquot was cryopreserved. Before cryopreservation, sperm quality parameters (concentration, motility, and viability) were evaluated. After thawing, sperm motility, plasma membrane integrity, lipid disorder, mitochondrial membrane potential, reactive oxygen species (ROS), and calcium intracellular levels were also determined. Based on the percentages of total motility (TM) and of sperm with an intact plasma membrane (SYBR14+/PI−) after thawing, samples were classified as good-freezability (GFE) or poor-freezability (PFE) ejaculates through cluster analyses. The SP activity levels of enzymatic (SOD and PON1) and non-enzymatic antioxidants (CUPRAC, FRAP, and TEAC) were higher (p < 0.05) in GFE than in PFE, whereas SP-OSI was higher (p < 0.05) in PFE than in GFE. In addition, the activity levels of SOD, PON1, GPX, CUPRAC, FRAP, and TEAC were positively (p < 0.05) related to post-thaw sperm motility and plasma membrane integrity and negatively to intracellular ROS levels. The SP-OSI was negatively correlated (p < 0.05) to post-thaw sperm quality parameters and positively to intracellular ROS levels. It can thus be concluded that donkey SP antioxidants are related to sperm cryotolerance and that measurements of antioxidants PON1, SOD, CUPRAC, FRAP, and TEAC, as well as SP-OSI, could be used as markers of sperm cryotolerance. Further research addressing the relationship of these antioxidants and SP-OSI with sperm cryotolerance and their potential use as freezing markers is warranted.

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Section: Introductionmentioning

confidence: 99%

Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance

et al. 2022

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“…Yet, the removal of SP increases the degree of sperm susceptibility to oxidative damage, as the enzymatic and non-enzymatic antioxidants that this fluid contains are also removed [ 29 , 31 , 32 ]. Moreover, in horses, as in other mammalian species, there is great variability between, and even within, stallions in the ability of their sperm to withstand cryopreservation [ 27 , 33 , 34 , 35 , 36 , 37 ], leading to the classification of ejaculates/stallions as having good freezability (GFE) or poor freezability (PFE) [ 28 , 30 , 35 , 38 ]. This variability could be related to differences in the composition of SPs and the activity exerted by their antioxidants [ 39 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen–Thawed Horse Sperm

Catalán,

Yánez-Ortiz,

Torres-Garrido

et al. 2024

Antioxidants

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Cryopreservation is a stressful process for sperm, as it is associated with an increased production of reactive oxygen species (ROS). Elevated ROS levels, which create an imbalance with antioxidant capacity, may result in membrane lipid peroxidation (LPO), protein damage and DNA fragmentation. This study aimed to determine whether the membrane LPO and DNA fragmentation of frozen–thawed horse sperm relies upon antioxidant activity, including enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and paraoxonase type 1 (PON1)); non-enzymatic antioxidant capacity (Trolox-equivalent antioxidant capacity (TEAC), plasma ferric reducing antioxidant capacity (FRAP) and cupric reducing antioxidant capacity (CUPRAC)); and the oxidative stress index (OSI) of their seminal plasma (SP). Based on total motility and plasma membrane integrity (SYBR14+/PI−) after thawing, ejaculates were hierarchically (p < 0.001) clustered into two groups of good- (GFEs) and poor-(PFEs) freezability ejaculates. LPO and DNA fragmentation (global DNA breaks) were higher (p < 0.05) in the PFE group than in the GFE group, with LPO and DNA fragmentation (global DNA breaks) after thawing showing a positive relationship (p < 0.05) with SP OSI levels and ROS production. In addition, sperm motility and membrane integrity after thawing were negatively (p < 0.05) correlated with the activity levels of SP antioxidants (PON1 and TEAC). The present results indicate that LPO and DNA fragmentation in frozen–thawed horse sperm vary between ejaculates. These differences could result from variations in the activity of antioxidants (PON1 and TEAC) and the balance between the oxidant and antioxidant components present in the SP.

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“…Yet, removal of SP increases the degree of sperm susceptibility to oxidative damage as the enzymatic and non-enzymatic antioxidants that this fluid contains are also removed [25][26][27]. Moreover, in horses, as in other mammalian species, there is great variability between, and even within, stallions in the ability of their sperm to withstand cryopreservation [28][29][30][31][32][33], leading to the classification of males/ejaculates as with "poor" (PFE) or "good" (GFE) cryotolerance [30,[34][35][36]. This variability could be related to differences in the composition of SP and the activity exerted by their antioxidants [37][38][39].…”

Section: Introductionmentioning

confidence: 99%

Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen‐Thawed Horse Sperm

Catalán,

Yánez-Ortiz,

Torres-Garrido

et al. 2023

Preprint

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Cryopreservation is a stressful process for sperm as it is associated with an increased production of reactive oxygen species (ROS). Elevated ROS levels, which create an imbalance with antioxidant capacity, may result in membrane lipid peroxidation (LPO), protein damage and DNA fragmentation. This study aimed to determine whether membrane LPO and DNA fragmentation of frozen-thawed horse sperm relies upon the antioxidant activity, including enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), and paraoxonase type 1 (PON1)); non-enzymatic antioxidant capacity (Trolox equivalent antioxidant capacity (TEAC), plasma ferric reducing antioxidant capacity (FRAP), and cupric reducing antioxidant capacity (CUPRAC)); and the oxidative stress index (OSI), of their seminal plasma (SP). Based on total motility and plasma membrane integrity (SYBR14+/PI−) after thawing, ejaculates were hierarchically (p &lt; 0.001) clustered into two groups of good (GFE) and poor (PFE) freezability. LPO and DNA fragmentation (global DNA breaks) were higher (p &lt; 0.05) in PFE than in GFE, with LPO and DNA fragmentation (global DNA breaks) after thawing showing a positive relationship (p &lt; 0.05) with SP-OSI levels and ROS production. In addition, sperm motility and membrane integrity after thawing were negatively (p &lt; 0.05) correlated with the activity levels of SP antioxidants (PON1 and TEAC). The present results indicate that LPO and DNA fragmentation in frozen-thawed horse sperm vary between ejaculates. These differences could result from variations in the activity of antioxidants (PON1 and TEAC) and the balance between the oxidant and antioxidant components present in the SP.

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Selección Espermática en Semen Congelado/Descongelado de Equino: Evaluación de las Membranas Plasmática, Acrosomal y Potencial de Membrana Mitocondrial

Cited by 5 publications

References 19 publications

Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance

Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance

Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen–Thawed Horse Sperm

Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen‐Thawed Horse Sperm

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