Improved cell sensitivity and longevity in a rapid impedance‐based toxicity sensor

Curtis, Theresa M.; Tabb, Joel; Romeo, Lori E.; Schwager, Steven J.; Widder, Mark W.; Schalie, William H. van der

doi:10.1002/jat.1421

Cited by 48 publications

(31 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…If 6°C storage is not available, then cell lines with different optimal storage temperatures could be utilized and tailored for the testing environment. It has been shown that fish and reptilian cell lines have toxicant sensitivities comparable to mammalian cell lines (Brennan et al, 2012;Curtis et al, 2009a); indicating that a wide variety of vertebrate cells could be used as accurate predictors of human toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…Impedance measurements of cells on the open well chips and fluidic biochips were monitored using the ECIS 1600 analyzer (Applied BioPhysics, Troy, NY) as previously described by Curtis et al (2009a). Table 2 summarizes the cells performance in open well ECIS chips.…”

Section: Impedance Measurement With Ecismentioning

confidence: 99%

“…A wide array of analytespecific assays exists for detection of chemicals in water (Kelly et al, 2008;Pancrazio et al, 1999;Reardon et al, 2009;States et al, 2003), but a large number of assays would be needed to detect all possible chemicals, and unknown toxic chemicals would still escape detection. To meet this growing need, a variety of toxicity sensors have been developed that can detect a broad range of chemical contaminants (Curtis et al, 2009a;Eltzov and Marks, 2010;Iuga et al, 2009, andO'Shaughnessy et al, 2004). One toxicity sensor that shows great potential for low cost, maintenancefree detection of many chemical contaminants is ECIS sensing of vertebrate cells, which was first described by Giaever and Keese (1992), and further developed by Curtis et al (2009a,b), Brennan et al (2012).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Suitability of Invertebrate and Vertebrate Cells in a Portable Impedance-Based Toxicity Sensor: Temperature Mediated Impacts on Long-Term Survival

Curtis¹,

Collins²,

Gerlach³

et al. 2013

Self Cite

View full text Add to dashboard Cite

Standard Form 298 (Rev. 8/98) REPORT DOCUMENTATION PAGEPrescribed by ANSI Std. Z39.18 Form Approved OMB No. 0704-0188The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing the burden, Using ECIS (electric cell-substrate impedance sensing) to monitor the impedance of vertebrate cell monolayers provides a sensitive measure of toxicity for a wide range of chemical toxicants. One major limitation to using a cell-based sensor for chemical toxicant detection in the field is the difficulty in maintaining cell viability over extended periods of time prior to use. This research was performed to identify cell lines suitable for ECIS-based toxicity sensing under field conditions. A variety of invertebrate and vertebrate cell lines were screened for their abilities to be stored for extended periods of time on an enclosed fluidic biochip with minimal maintenance. Three of the ten cell lines screened exhibited favorable portability characteristics on the biochips. Interestingly, all three cell lines were derived from ectothermic vertebrates, and the storage temperature that allowed long-term cell survival on the enclosed fluidic biochips was also at the lower end of reported body temperature for the organism. Future work with the ectothermic vertebrate cells will characterize their sensitivity to a wide range of chemical toxicants. Using ECIS (electric cell-substrate impedance sensing) to monitor the impedance of vertebrate cell monolayers provides a sensitive measure of toxicity for a wide range of chemical toxicants. One major limitation to using a cell-based sensor for chemical toxicant detection in the field is the difficulty in maintaining cell viability over extended periods of time prior to use. This research was performed to identify cell lines suitable for ECIS-based toxicity sensing under field conditions. A variety of invertebrate and vertebrate cell lines were screened for their abilities to be stored for extended periods of time on an enclosed fluidic biochip with minimal maintenance. Three of the ten cell lines screened exhibited favorable portability characteristics on the biochips. Interestingly, all three cell lines were derived from ectothermic vertebrates, and the storage temperature that allowed long-term cell survival on the enclosed fluidic biochips was also at the lower end of reported body temperature for the organism, suggesting that reduced cellular metabolism may be essential for longterm survival on the biochip. Future work with the ectothermic vertebrate cells will characterize their sensitivity to a wide range of chemical toxicants to determine if they are good candidates for use in a field portable toxicity sensor.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Impedance Measurement With Ecismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Suitability of Invertebrate and Vertebrate Cells in a Portable Impedance-Based Toxicity Sensor: Temperature Mediated Impacts on Long-Term Survival

Curtis¹,

Collins²,

Gerlach³

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cellular impedance has been shown to be a sensitive indicator of cell viability and cytotoxicity (Giaever and Keese, 1993;Curtis et al, 2009a;Keese et al, 1998;Xing et al, 2005Xing et al, , 2006. Other investigators have found excellent correlation between cellular impedance and other toxicity endpoints such as tetrazolium dye and neutral red uptake assays (Xing et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Long-term storage and impedance-based water toxicity testing capabilities of fluidic biochips seeded with RTgill-W1 cells

Brennan

Widder

Lee

et al. 2012

Toxicology in Vitro

Self Cite

View full text Add to dashboard Cite

Widder, Mark W.; Lee, Lucy E.J.; and van der Schalie, William H., "Long-term storage and impedance-based water toxicity testing capabilities of fluidic biochips seeded with RTgill-W1 cells" (2012 Rainbow trout gill epithelial cells (RTgill-W1) are used in a cell-based biosensor that can respond within one hour to toxic chemicals that have the potential to contaminate drinking water supplies. RTgill-W1 cells seeded on enclosed fluidic biochips and monitored using electric cell-substrate impedance sensing (ECIS) technology responded to 18 out of the 18 toxic chemicals tested within one hour of exposure. Nine of these chemical responses were within established concentration ranges specified by the U.S. Army for comparison of toxicity sensors for field application. The RTgill-W1 cells remain viable on the biochips at ambient carbon dioxide levels at 6°C for 78 weeks without media changes. RTgill-W1 biochips stored in this manner were challenged with 9.4 lM sodium pentachlorophenate (PCP), a benchmark toxicant, and impedance responses were significant (p < 0.001) for all storage times tested. This poikilothermic cell line has toxicant sensitivity comparable to a mammalian cell line (bovine lung microvessel endothelial cells (BLMVECs)) that was tested on fluidic biochips with the same chemicals. In order to remain viable, the BLMVEC biochips required media replenishments 3 times per week while being maintained at 37°C. The ability of RTgill-W1 biochips to maintain monolayer integrity without media replenishments for 78 weeks, combined with their chemical sensitivity and rapid response time, make them excellent candidates for use in low cost, maintenance-free field-portable biosensors.Published by Elsevier Ltd.

show abstract

“…One of the main reasons for the recent success is the development of cellbased sensor systems which can be used in field applications [21]. In this context, Electrical cell impedance spectroscopy [22] has been demonstrated to be feasible to monitor various aquatic toxicants including heavy metals and industrial pollutants [23]. Cr(VI) detection using cell-based impedance sensors was already performed before, using the Real-Time Cell Electronic Sensor [24].…”

Section: Introductionmentioning

confidence: 99%

A critical comparison of cell-based sensor systems for the detection of Cr(VI) in aquatic environment

Bohrn

Mucha

Werner

et al. 2013

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

a b s t r a c tThe toxicity of chromium ions was investigated using mammalian cell cultures on impedance sensors as well as physiological in vitro sensor systems. The performance of commercially available systems like the 2500 Analyzing System (Bionas), xCELLigence (Roche) and Cytosensor Microphysiometer (Molecular Devices) was compared with a novel CMOS-based impedance-to-frequency converter device. Cell-based sensor systems are shown to be powerful tools to detect Cr(VI) pollutions within several hours in the range of multinational drinking water regulations. The ability to distinguish between toxic Cr(VI) and non-toxic Cr(III) species is one advantage of these integral sensor systems. Impedance only devices are not sufficient for the fast detection of toxic chromium species as rapid cellular changes occur only in the respiration system and the cell physiology.

show abstract

Improved cell sensitivity and longevity in a rapid impedance‐based toxicity sensor

Cited by 48 publications

References 23 publications

Suitability of Invertebrate and Vertebrate Cells in a Portable Impedance-Based Toxicity Sensor: Temperature Mediated Impacts on Long-Term Survival

Suitability of Invertebrate and Vertebrate Cells in a Portable Impedance-Based Toxicity Sensor: Temperature Mediated Impacts on Long-Term Survival

Long-term storage and impedance-based water toxicity testing capabilities of fluidic biochips seeded with RTgill-W1 cells

A critical comparison of cell-based sensor systems for the detection of Cr(VI) in aquatic environment

Contact Info

Product

Resources

About