Impregnation of Oral Mucosa Over Impacted Teeth by Subpopulations of Macrophages M1 and M2

Abstract

The aim: Of the study is to research quantitative parameters of mucous membrane macrophages populations M1 (CD68+) and M2 (CD163+) over vestibularly and palatally impacted teeth. Materials and methods: A group of 21 people aged from 10 to 16 years was formed to conduct the research. Clinical situation according to diagnostic criteria was identical in all the patients. The group was divided into two groups - control and experimental, which in their turn were fragmented into two subgroups. Immunohistochemical studies of mucosal biopsies were performed in accordance with the recommendations for selection. Results: Study of ratio of CD68+/CD163+ cells revealed imbalance in individuals with vestibularly impacted teeth due to higher infiltration density of CD163+ (p<0,05), compared to CD68+ of control group. In individuals with palatally impacted teeth, ratio of CD68+/CD163+ increased 3,6 times, as well as compared with control group, but due increased infiltration density of CD68+. Conclusions: In the epithelium of oral mucosa located over impacted teeth, both on vestibular and palatal surface, number of CD 68+ and CD163+ cells had no significant differences compared to control group. In biopsies of the lamina propria of mucosa over vestibularly impacted teeth, the ratio M1/M2=0,91±0,11 (p<0,05) decreases, with predominance of macrophages CD163+ subpopulation activity, and over palatally impacted teeth balance of M1/ M2 macrophages elevated (M1/M2= 2,10 ± 0,32, p<0,05), due to increased infiltration density of CD68+.

Retention of Mandibular Molars: Etiological Factors, Current Diagnostic Approaches, and Treatment Options

“…Схожого висновку дійшла і Дмитренко М.І. 2021р у своїх дослідженнях стосовно ролі прозапальних М1 (CD68+) і протизапальних М2 (CD163+) популяцій макрофагів у слизовій оболонці над ретенованими зубами [23].…”

“…На етапі планування техніки відкриття слизової оболонки над коронками ретенованих зубів необхідно враховувати особливості їх розташування та дотримуватись показань у кожному окремому випадку задля уникнення помилок, ускладнень та отримати необхідний клінічний результат [23,27]. Тому методи створення хірургічного доступу для оптимізації ортодонтичного переміщення ретенованих молярів потребують постійного вдосконалення.…”

Dynamics of CD68 receptor expression in macrophages of the interstitial space of the rat testis under triptorelin prolonged administration

“…Testosterone has a considerable impact on the functional state of macrophages in the body. It inhibits the macrophages polarization according to the proinflammatory phenotype (M1), which is manifested in a decrease in the production of such cytokines as interleukin 1β (IL-1 β) and interleukin 6 (IL-6) [5,6].…”