The aim of the study was to give a morphometric characterization of hepatocytes in acute experimental aseptic peritonitis in rats. The study was performed on 50 adult rats weighing 180-200 g, divided into two groups: 1st intact animals (5), 2nd animals (45), which were simulated acute experimental aseptic peritonitis by intraperitoneal injection of 5 mg λ – carrageenan, which was diluted in 1 ml of isotonic sodium chloride solution per 1 animal. Withdrawal of animals from the experiment occurred on the 1st, 2nd, 3rd, 5th, 7th, 10th, 14th, 21st and 30th days. Paraffin sections were made according to generally accepted methods. Analysis of hepatocyte morphometric parameters was performed according to generally accepted statistical methods using Microsoft Office Excel 2007, the probability of differences in quantitative results was determined using Student's t-test, which were considered statistically significant in the common in medical and biological examinations error probability p˂0,05. It was found that acute experimental aseptic peritonitis occurred from the first days of the experiment. Significant changes in the structure of hepatocytes were detected gradually in the following days, and the terminal phase of inflammation developed on the 7th-10th day of the experiment. The large diameter of hepatocytes decreased and on the 30th day did not reach the size of the intact group, the small diameter increased slightly and also on the 30th day of the experiment did not reach the values of the intact group of animals. There was a slight decrease in the area of hepatocytes, while the nuclear cytoplasmic index had a slight increase. From the 1st day of the experiment, the average size of the nuclei of hepatocytes partially increased. The correlation between large diameter and cell area found in the study was directly weak. There was also a direct correlation between the area and large diameter of hepatocyte nuclei. The obtained data of morphometric parameters of hepatocytes suggest that acute experimental aseptic peritonitis may cause a state of functional stress of hepatocytes in the animal.

Testosterone, the production of which is stimulated by the release of luteinizing hormone (LH) has a remarkable anti-inflammatory and immunomodulatory effect, and in conditions of testosterone deficiency tissue damage can occur due to excessive differentiation of macrophages into a pro-inflammatory M1 phenotype. The aim of this study was to determine the spread of CD68 receptorn as the marker of inflammation on the cells in the interstitial space and testicular vessels under LH synthesis blockade with tryptorelin. Sexually mature white male rats were randomly divided into control (10 animals) and experimental (25 animals) groups. Animals in the experimental group were injected with a tryptorelin acetate solution (0.3 mg/kg). Immunochemical analysis of CD68+ expression was estimated at Olympus FV10i-LIV laser scanning confocal microscope using fluorescent labeling dye hilyte flour 488. It was shown that luteinizing hormone deprivation led to an increase in the distribution of the CD68 receptor in the interstitial space and in the testicular vessels from day 30 to 180 of the experiment, associated with the increase of inducible NO synthase activity in testis tissue. Keywords: CD68, luteinizing hormone, macrophage M1 phenotype, testis, testosterone, tryptorelin References: