Importing Mitochondrial Proteins: Machineries and Mechanisms

Chacińska, Agnieszka; Koehler, Carla M.; Milenkovic, Dusanka; Lithgow, Trevor; Pfanner, Nikolaus

doi:10.1016/j.cell.2009.08.005

Cited by 1,273 publications

(1,388 citation statements)

References 129 publications

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“…All mitochondrial matrix proteins are encoded by nuclear genes and are transported across the outer and inner mitochondrial membrane after being synthesized by cytoplasmic ribosomes ( (Attardi and Schatz, 1988;Chacinska et al, 2009;Lemire et al, 1989;Schmidt et al, 2010). Similar to other proteins destined for the matrix, AfLEA1.3 contains a positively charged N-terminal pre-sequence; the projected cleavage site is after amino-acid position 36 but has not been experimentally confirmed.…”

Section: Discussionmentioning

confidence: 99%

Improved tolerance to salt and water stress in Drosophila melanogaster cells conferred by late embryogenesis abundant protein

Marunde

Samarajeewa

Anderson

et al. 2013

Journal of Insect Physiology

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Graphical AbstractHighlights: ØThe Late Embryogenesis Abundant protein AfLEA1.3 from Artemia franciscana accumulates in the mitochondrion of Drosophila Kc167 cells. Ø AfLEA1.3 improves mitochondrial functions in presence of high sodium chloride concentrations. Ø AfLEA1.3 reduces mitochondrial damage during freeze-thawing. Ø AfLEA1.3 increases cellular tolerance to osmotic stress. Ø AfLEA1.3 increases cellular tolerance to convective drying.

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Section: Discussionmentioning

confidence: 99%

Improved tolerance to salt and water stress in Drosophila melanogaster cells conferred by late embryogenesis abundant protein

Marunde

Samarajeewa

Anderson

et al. 2013

Journal of Insect Physiology

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“…Tom20 and Tom22 play a major role in the import of presequence-carrying proteins into mitochondria, whereas the third receptor Tom70 is important for importing non-cleavable carrier proteins [2,3]. To study whether Tom20 and Tom22 were involved in the import of Om45, we incubated the 35 S-labelled precursor of Om45 with isolated mitochondria.…”

Section: Om45 Is Imported Via the Tom Complexmentioning

confidence: 99%

The presequence pathway is involved in protein sorting to the mitochondrial outer membrane

et al. 2014

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The mitochondrial outer membrane contains integral a-helical and b-barrel proteins that are imported from the cytosol. The machineries importing b-barrel proteins have been identified, however, different views exist on the import of a-helical proteins. It has been reported that the biogenesis of Om45, the most abundant signal-anchored protein, does not depend on proteinaceous components, but involves direct insertion into the outer membrane. We show that import of Om45 occurs via the translocase of the outer membrane and the presequence translocase of the inner membrane. Assembly of Om45 in the outer membrane involves the MIM machinery. Om45 thus follows a new mitochondrial biogenesis pathway that uses elements of the presequence import pathway to direct a protein to the outer membrane.

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“…A typical metazoan mitogenome consists of a single circular DNA that carries 13 intronless protein coding genes, the two subunits of the mitochondrial ribosomal RNA, and 22 tRNAs, one for each amino acid except leucine and serine, which have two copies each. Almost their complete proteome, much of which is of prokaryotic origin, is imported from the nucleus (Chacinska et al, 2009;Huynen, 2010).…”

Section: Introductionmentioning

confidence: 99%