Improved tolerance to salt and water stress in Drosophila melanogaster cells conferred by late embryogenesis abundant protein

Marunde, Matthew R.; Samarajeewa, Dilini A; Anderson, John M.; Li, Shumin; Hand, Steven C.; Menze, Michael A.

doi:10.1016/j.jinsphys.2013.01.004

Cited by 40 publications

(34 citation statements)

References 70 publications

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“…A similar case is seen in rotifers, where two LEA mRNAs are very similar but arise from two individual genes on different chromosomes (Pouchkina-Stantcheva et al 2007). In addition, very similar variants of group 1 LEA proteins have been documented in A. franciscana and attributed to independent genes (Sharon et al 2009;Warner et al 2010;Warner et al 2012;Marunde et al 2013). All four of the mitochondrial AfrLEA3m proteins migrate with an apparent molecular mass 1.12-1.34 times higher than their deduced molecular masses.…”

Section: Discussionsupporting

confidence: 55%

“…To date, all LEA proteins described from animals have been assigned to group 3 (for classification scheme, see Wise 2003), with the exception of group 1 LEA proteins discovered in A. franciscana (Sharon et al 2009;Warner et al 2010;Wu et al 2011;Marunde et al 2013). Group 3 LEA proteins are predicted to have high alpha-helix content, but have been found experimentally to be unfolded when fully hydrated in aqueous solution (Goyal et al 2003).…”

Section: Introductionmentioning

confidence: 99%

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Quantification of cellular protein expression and molecular features of group 3 LEA proteins from embryos of Artemia franciscana

Boswell

Moore

Hand

2014

Cell Stress and Chaperones

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Late embryogenesis abundant (LEA) proteins are highly hydrophilic, low complexity proteins whose expression has been correlated with desiccation tolerance in anhydrobiotic organisms. Here, we report the identification of three new mitochondrial LEA proteins in anhydrobiotic embryos of Artemia franciscana, AfrLEA3m_47, AfrLEA3m_43, and AfrLEA3m_29. These new isoforms are recognized by antibody raised against recombinant AfrLEA3m, the original mitochondrial-targeted LEA protein previously reported from these embryos; mass spectrometry confirms all four proteins share sequence similarity. The corresponding messenger RNA (mRNA) species for the four proteins are readily amplified from total complementary DNA (cDNA) prepared from embryos. cDNA sequences of the four mRNAs are quite similar, but each has a stretch of sequence that is absent in at least one of the others, plus multiple single base pair differences. We conclude that all four mitochondrial LEA proteins are products of independent genes. Each possesses a mitochondrial targeting sequence, and indeed Western blots performed on extracts of isolated mitochondria clearly detect all four isoforms. Based on mass spectrometry and sodium dodecyl sulfate polyacrylamide gel electrophoresis migration, the cytoplasmic-localized AfrLEA2 exists primarily as a homodimer in A. franciscana. Quantification of protein expression for AfrLEA2, AfrLEA3m, AfrLEA3m_43, and AfrLEA3m_29 as a function of development shows that cellular concentrations are highest in diapause embryos and decrease during development to low levels in desiccationintolerant nauplius larvae. When adjustment is made for mitochondria matrix volume, the effective concentrations of cytoplasmic versus mitochondrial group 3 LEA proteins are similar in vivo, and the values provide guidance for the design of in vitro functional studies with these proteins.

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Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Quantification of cellular protein expression and molecular features of group 3 LEA proteins from embryos of Artemia franciscana

Boswell

Moore

Hand

2014

Cell Stress and Chaperones

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“…AfLEA1.3 preserves mitochondrial function and improves survival of transgenic Drosophila melanogaster Kc167 cells during freeze-thaw, drying, and hyperosmosis. AfLEA1.3 protects against drying when hydrated and unstructured, showing that folding is not a prerequisite for LEA protein activity (Marunde et al 2013).…”

Section: Lea Proteins and Desiccation Tolerance In Artemia Cystsmentioning

confidence: 99%

Stress tolerance during diapause and quiescence of the brine shrimp, Artemia

MacRae

2016

Cell Stress and Chaperones

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Oviparously developing embryos of the brine shrimp, Artemia, arrest at gastrulation and are released from females as cysts before entering diapause, a state of dormancy and stress tolerance. Diapause is terminated by an external signal, and growth resumes if conditions are permissible. However, if circumstances are unfavorable, cysts enter quiescence, a dormant stage that continues as long as adverse conditions persist. Artemia embryos in diapause and quiescence are remarkably resistant to environmental and physiological stressors, withstanding desiccation, cold, heat, oxidation, ultraviolet radiation, and years of anoxia at ambient temperature when fully hydrated. Cysts have adapted to stress in several ways; they are surrounded by a rigid cell wall impermeable to most chemical compounds and which functions as a shield against ultraviolet radiation. Artemia cysts contain large amounts of trehalose, a non-reducing sugar thought to preserve membranes and proteins during desiccation by replacing water molecules and/or contributing to vitrification. Late embryogenesis abundant proteins similar to those in seeds and other anhydrobiotic organisms are found in cysts, and they safeguard cell organelles and proteins during desiccation. Artemia cysts contain abundant amounts of p26, a small heat shock protein, and artemin, a ferritin homologue, both ATPindependent molecular chaperones important in stress tolerance. The evidence provided in this review supports the conclusion that it is the interplay of these protective elements that make Artemia one of the most stress tolerant of all metazoan organisms.

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“…In addition, in vitro and in vivo experiments have shown that these components are necessary but not sufficient to acquire complete desiccation tolerance. Therefore, whole-genome screening for anhydrobiosis-related features became an obligatory step in further understanding the molecular background of successful desiccation tolerance1415.…”

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confidence: 99%

Comparative genome sequencing reveals genomic signature of extreme desiccation tolerance in the anhydrobiotic midge

et al. 2014

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Anhydrobiosis represents an extreme example of tolerance adaptation to water loss, where an organism can survive in an ametabolic state until water returns. Here we report the first comparative analysis examining the genomic background of extreme desiccation tolerance, which is exclusively found in larvae of the only anhydrobiotic insect, Polypedilum vanderplanki. We compare the genomes of P. vanderplanki and a congeneric desiccation-sensitive midge P. nubifer. We determine that the genome of the anhydrobiotic species specifically contains clusters of multi-copy genes with products that act as molecular shields. In addition, the genome possesses several groups of genes with high similarity to known protective proteins. However, these genes are located in distinct paralogous clusters in the genome apart from the classical orthologues of the corresponding genes shared by both chironomids and other insects. The transcripts of these clustered paralogues contribute to a large majority of the mRNA pool in the desiccating larvae and most likely define successful anhydrobiosis. Comparison of expression patterns of orthologues between two chironomid species provides evidence for the existence of desiccation-specific gene expression systems in P. vanderplanki.

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Improved tolerance to salt and water stress in Drosophila melanogaster cells conferred by late embryogenesis abundant protein

Cited by 40 publications

References 70 publications

Quantification of cellular protein expression and molecular features of group 3 LEA proteins from embryos of Artemia franciscana

Quantification of cellular protein expression and molecular features of group 3 LEA proteins from embryos of Artemia franciscana

Stress tolerance during diapause and quiescence of the brine shrimp, Artemia

Comparative genome sequencing reveals genomic signature of extreme desiccation tolerance in the anhydrobiotic midge

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