Importância de teste de DNA para verificação de parentesco em Búfalos (Bubalus bubalis)

Vieira, J. N.; Teixeira, Cristina Mara; Kuabara, Marcelo Yukio; Oliveira, Denise Aparecida Andrade de

doi:10.22256/pubvet.v5n2.1004

Cited by 3 publications

(4 citation statements)

References 4 publications

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“…Moreover, results of many studies on Indian and Guilan buffalo breeds revealed also high values of PIC (0.52–0.88) for most studied markers [ 14 , 15 , 65 , 66 ]. Other researchers [ 10 , 12 , 67 , 68 , 69 , 70 , 71 , 72 ] reported varied values of PIC for several markers which ranging between moderate (0.34–0.48) and high (0.51–0.82). The PIC values variability observed in the literature may be due to different microsatellite markers used in the studied populations.…”

Section: Discussionmentioning

confidence: 95%

Evaluation of Genetic Diversity and Structure of Turkish Water Buffalo Population by Using 20 Microsatellite Markers

Ünal

Işık

Şen

et al. 2021

Animals

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The present study was aimed to investigate the genetic diversity among 17 Turkish water buffalo populations. A total of 837 individuals from 17 provincial populations were genotyped, using 20 microsatellites markers. The microsatellite markers analyzed were highly polymorphic with a mean number of alleles of (7.28) ranging from 6 (ILSTS005) to 17 (ETH003). The mean observed and expected heterozygosity values across all polymorphic loci in all studied buffalo populations were 0.61 and 0.70, respectively. Observed heterozygosity varied from 0.55 (Bursa (BUR)) to 0.70 (Muş (MUS)). It was lower than expected heterozygosity in most of the populations indicating a deviation from Hardy–Weinberg equilibrium. The overall value for the polymorphic information content of noted microsatellite loci was 0.655, indicating their suitability for genetic diversity analysis in buffalo. The mean FIS value was 0.091 and all loci were observed significantly deviated from Hardy–Weinberg Equilibrium (HWE), most likely based on non-random breeding. The 17 buffalo populations were genetically less diverse as indicated by a small mean FST value (0.032 ± 0.018). The analysis of molecular variance (AMOVA) analysis indicated that about 2% of the total genetic diversity was clarified by population distinctions and 88 percent corresponded to differences among individuals. The information produced by this study can be used to establish a base of national conservation and breeding strategy of water buffalo population in Turkey.

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Section: Discussionmentioning

confidence: 95%

Evaluation of Genetic Diversity and Structure of Turkish Water Buffalo Population by Using 20 Microsatellite Markers

Ünal

Işık

Şen

et al. 2021

Animals

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“…Results of many studies on Indian and Guilan buffalo breeds showed also high values of PIC (0.52-0.88) for most studied markers (Tantia et al, 2006;Aminafshar et al, 2008;Kataria et al, 2009;Jakhesara et al, 2010;Kathiravan et al, 2012;Singh et al, 2017). Other researchers (Arora et al, 2004;Ángel-Marín et al, 2010;Bhuyan et al, 2010;Vieira et al, 2011;Rad et al, 2013;Ünal et al, 2014) reported varied values of PIC for several markers which ranging between moderate (0.34-0.48) and high (0.51-0.82).…”

Section: Polymorphic Information Content (Pic)mentioning

confidence: 92%

“…A total of 15 microsatellite markers were chosen for this study from the cattle genome based on their high polymorphism, polymorphic information content (PIC) with good heterozygosity and information available from previous studies in buffalo (Moore et al, 1995;Barker et al, 1997;Moioli et al, 2001;Tantia et al, 2006;Elbeltagy et al, 2008;Zhang et al, 2008;Bhuyan et al, 2010;Jakhesara et al, 2010;Marques et al, 2011;Vieira et al, 2011;Acosta et al, 2014;Ünal et al, 2014). These microsatellites were BMC1013, CSSM019, CSSM022, CSSM029, CSSM036, CSSM038, CSSM041, CSSM043, CSSM045, CSSM046, CSSM047, ETH3 (D19S2), BM1818, ILSTS005, and ILSTS33.…”

Section: Selection Of Microsatellites Markers and Pcr Amplificationmentioning

confidence: 99%

Genetic Diversity Analysis of Five Egyptian Buffalo Populations Using Microsatellite Markers

Merdan¹,

El-Zarei²,

Ghazy³

et al. 2020

JAS

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For assessing the genetic diversity and genetic characterization of five Egyptian buffalo populations a total of 12 microsatellite markers were used. The total number of buffaloes sampled was 80, collected at random from five farms in five different governorates; Cairo, Kafr El-Sheikh, Shebeen El-Kom, Menoufia, and Beni Suef. The genetic parameters (allelic diversity, allelic frequencies, observed heterozygosity, unbiased expected heterozygosity, and polymorphic information content) were calculated using three different programs. All used microsatellites were polymorphic and ranged from four alleles (Loci; CSSM029, CSSM036, CSSM038, CSSM043, CSSM046, and ILSTS005) to nine alleles (Loci; BM1818 and CSSM047) with a total of 64 alleles in the whole population. Allelic richness for the whole population ranged between 3.297 (in locus CSSM029) and 6.806 (in locus CSSM047) with overall mean 4.574. Within populations, Kafr El-Sheikh population had the highest average of allelic richness (4.384). This indicates the potential of this population to adapt with environmental changes in future compared with other populations. BMC1013, BM1818, CSSM019, and CSSM047 showed the highest allelic richness. PIC estimates were high and ranged between 0.65 (in locus CSSM029) and 0.92 (in locus CSSM022) with an average of 0.82. Values of H o were lower than values of H Nb for all populations, which denoting depression of heterozygotes in these populations and may be attributable to existence of null alleles and inbreeding. This study as well proves the usefulness of heterologous bovine microsatellite markers in evaluation of the genetic variability in Egyptian buffalo populations due to high polymorphism, informativeness of these markers which can be used to develop future breeding strategies and conservation decisions on our indigenous breed.

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“…Silva et al (2014) propuseram um painel com 16 marcadores microssatélites para teste de paternidade em caprinos locais e comerciais, PE1 e PE2 de 99,98% e 99,99%. Vieira et al (2011) desenvolveu um painel para teste de paternidade em búfalos com 13 marcadores microssatélites para identificação genética e de paternidade em búfalos, o que se demonstrou eficiente.…”

Section: Discussionunclassified

Painel SRT para teste de paternidade em caprinos

et al. 2018

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Acasalamentos duvidosos são uma das causas dos erros nos programas de conservação e de melhoramento genético de raças. Os testes de paternidades são ferramentas que podem contribuir para minimizar esses erros pois tem sido usados para identificar paternidade duvidosa. O presente trabalho teve como objetivo definir um painel para teste de paternidade em caprinos para auxiliar programas de conservação e melhoramento de raças. Foram genotipados 381 animais de 10 populações caprinas sendo seis ecotipos locais brasileiros e quatro raças exóticas utilizadas atualmente no Brasil (Alpina, Anglo-Nubiana, Boer e Saanen). Realizou-se nove sistemas multiplex, totalizando 27 microssatélites. Foram estimados o PIC, as probabilidades de exclusão dos marcadores e o equilíbrio de Hardy-Weinberg (EHW) nas populações, seguida de determinação da probabilidade de identidade (PI). Para o sistema de 27 microssatélites a probabilidade de exclusão combinada (PE) foi de 0,999 e 0,999 (PE1 e PE2) e, 21 microssatélites apresentaram PIC  0,60, onde quatro apresentaramse monomórficos em algumas populações. Para compor o novo painel foram escolhidos 9 marcadores dos quais oito comuns aos dois grupos estudados (caprinos locais e exóticos), sendo o BM1818 mais informativo nas populações locais e, o marcador BM6506 nas populações exóticas. A probabilidade de exclusão combinada com o grupo dos microssatélites foi de 0,994 e 0,999 (PE1 e PE2). Baseado no cálculo das probabilidades de identidade dentro das populações foi possível discriminar um indivíduo entre um milhão (106). Os resultados indicaram a possibilidade de empregar poucos microssatélites na investigação de paternidade em caprinos, com alto grau de confiabilidade, minimizando assim custos para o criador.

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Importância de teste de DNA para verificação de parentesco em Búfalos (Bubalus bubalis)

Cited by 3 publications

References 4 publications

Evaluation of Genetic Diversity and Structure of Turkish Water Buffalo Population by Using 20 Microsatellite Markers

Evaluation of Genetic Diversity and Structure of Turkish Water Buffalo Population by Using 20 Microsatellite Markers

Genetic Diversity Analysis of Five Egyptian Buffalo Populations Using Microsatellite Markers

Painel SRT para teste de paternidade em caprinos

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