Various studies provide evidence for a phonological priming effect in the recognition of single signs based on phonological parameters, i.e., handshape, location and movement. In addition, some of these studies show that phonological parameters influence this effect differently. The current eye tracking study on German Sign Language examined the presence of a phonological priming effect at the sentence level depending on the phonological relation of prime-target sign pairs. We recorded participants’ eye movements while presenting a video of sentences containing either related or unrelated prime-target sign pairs, and a picture of the target and the distractor. The data provided evidence for a phonological priming effect for sign pairs sharing handshape and movement while differing in location. Moreover, a difference between parameters in their contribution to sign recognition was suggested such that recognition was facilitated for signs sharing handshape, but was inhibited for signs sharing location. Showing that sub-lexical features influence sign language processing.