Implications of using different tissue ferritins as antigens for ferritin in serum: four radioimmunoassay kits compared.

Oost, B.A. van; Willekens, F. L. A.; Neerbos, B R van; Beld, Bep van den

doi:10.1093/clinchem/28.12.2429

Cited by 7 publications

(3 citation statements)

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“…This is probably because of the distinct reactivity of Ferritin to the various kits used, antibody types, other molecules that potentially interfere with the assays, and the ratio of L and H chains of Ferritin [ 8 , 12 ]. The most commonly used commercial kits measure basic isoferritin that is rich in L chains, such as PF [ 13 ], whereas measurement of acid or neutral isoferritin such as SF may be inadequate because of the lack of specific commercial kits [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of systemic inflammation on level of ferritin seminal in chronic renal male patient undergoing hemodialysis

Silva¹,

Elena²,

Carneiro³

et al. 2014

Int Arch Med

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BackgroundMost hemodialysis patients present with chronic systemic inflammation characterized by the elevation of serum C-reactive protein (CRP) levels and/or the production of proinflammatory interleukins by the immune system in response to the hemodialysis process. Plasma ferritin(PF) is one of the parameters used to correct anemia. An PF level of >500 ng/mL is not recommended for correction of anemia because of the uncertainty of whether these levels are elevated because of anemia or a mere reaction to inflammation. we aimed to study the effects of inflammation on seminal ferritin (SF) levels and hypothesized that SF is not affected because of the testicular immune privilege.MethodsA prospective prevalence study was conducted at the Department of Hemodialysis of the University Hospital of Brasília (HuB) between June 2010 and July 2011. The sample included 60 chronic renal patients undergoing hemodialysis and 20 control subjects from the health promotion general outpatient clinic. All participants were males aged 18–60 years. Inflammation was assessed through serum CRP levels, and the testicular condition was determined by measuring sex hormone levels. In the patient group, inflammation was considered to be present when CRP was >5 mg/L (n = 27) and absent when CRP was ≤5 mg/L (n = 33). Control group (n = 20) CRP was ≤1 mg/L. Blood and semen were collected via arm venoclysis and after voluntary masturbation, respectively. CRP was measured by turbidimetry; PF, SF, and sex hormone levels by immunochemoluminescence. Statistical significance was set at p < 0.05.ResultsThere was no significant difference in mean SF levels among patients with inflammation (295.34 ± 145.39 ng/mL), those without inflammation (324.42 ± 145.51 mg/mL), and controls (335.70 ± 075.90 ng/mL; p = 0.49). There was no correlation between mean SF and PF levels in the patients with and without inflammation). All participants were eugonadal with mean serum FSH, LH, and testosterone levels of 3.76 ± 2.17 mUI/mL, 7.00 ± 3.53 mUI/mL, and 454.18 ± 173.08 ng/dL, respectively.ConclusionSystemic inflammation did not significantly alter SF levels in eugonadal hemodialysis patients.

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Section: Discussionmentioning

confidence: 99%

Effect of systemic inflammation on level of ferritin seminal in chronic renal male patient undergoing hemodialysis

Silva¹,

Elena²,

Carneiro³

et al. 2014

Int Arch Med

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“…Eleven thousand eight hundred ninety three records were obtained from the search strategy depicted in Table 1 . From 252 records extracted, 187 studies were included in the qualitative analysis [ 8 , 10 , 15 – 199 ] and 148 in the meta-analysis [ 8 , 10 , 15 , 17 , 18 , 22 , 23 , 26 , 28 , 30 – 32 , 34 – 56 , 58 – 64 , 66 – 68 , 72 , 73 , 75 , 77 – 80 , 82 – 85 , 87 – 92 , 94 – 98 , 100 – 102 , 106 – 109 , 111 – 116 , 120 – 137 , 139 – 143 , 145 , 146 , 148 – 151 , 153 – 157 , 159 – 164 , 166 – 175 , 177 , 179 – ...…”

Section: Resultsmentioning

confidence: 99%

“…Although it was not possible to perform comparisons or meta-analysis, available studies report the use of WHO or other international reference materials while developing and calibrating an assay, but also to improve comparability between methods and assays components [ 10 , 36 , 87 , 88 , 96 , 139 , 140 , 181 ]. Studies reporting on commercial, automated equipment refer the use of international reference materials for calibration while developing an assay [ 96 ].…”

Section: Discussionmentioning

confidence: 99%

Performance and comparability of laboratory methods for measuring ferritin concentrations in human serum or plasma: A systematic review and meta-analysis

et al. 2018

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BackgroundDifferent laboratory methods are used to quantify ferritin concentrations as a marker of iron status. A systematic review was undertaken to assess the accuracy and comparability of the most used methods for ferritin detection.Methods and findingsNational and regional databases were searched for prospective, retrospective, sectional, longitudinal and case-control studies containing the characteristics and performance of at least one method for serum/plasma ferritin determinations in humans published to date. The analysis included the comparison between at least 2 methods detailing: sensitivity, precision, accuracy, predictive values, inter-methods adjustment, and use of international reference materials. Pooled method performance was analyzed for each method and across methods.OutcomesSearch strategy identified 11893 records. After de-duplication and screening 252 studies were assessed, including 187 studies in the qualitative analysis and 148 in the meta-analysis. The most used methods included radiometric, nonradiometric and agglutination assays. The overall within-run imprecision for the most reported ferritin methods was 6.2±3.4% (CI 5.69–6.70%; n = 171), between-run imprecision 8.9±8.7% (CI 7.44–10.35%; n = 136), and recovery rate 95.6% (CI 91.5–99.7%; n = 94). The pooled regression coefficient was 0.985 among all methods analyzed, and 0.984 when comparing nonradiometric and radiometric methods, without statistical differences in ferritin concentration ranging from 2.3 to 1454 μμg/L.ConclusionThe laboratory methods most used to determine ferritin concentrations have comparable accuracy and performance. Registered in PROSPERO CRD42016036222.

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A sensitive time-resolved immunofluorometric assay of ferritin in serum with monoclonal antibodies

Khosravi¹,

Chan²,

Bellem³

et al. 1988

Clinica Chimica Acta

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Implications of using different tissue ferritins as antigens for ferritin in serum: four radioimmunoassay kits compared.

Cited by 7 publications

References 0 publications

Effect of systemic inflammation on level of ferritin seminal in chronic renal male patient undergoing hemodialysis

Effect of systemic inflammation on level of ferritin seminal in chronic renal male patient undergoing hemodialysis

Performance and comparability of laboratory methods for measuring ferritin concentrations in human serum or plasma: A systematic review and meta-analysis

A sensitive time-resolved immunofluorometric assay of ferritin in serum with monoclonal antibodies

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