Implications of Cellular Heterogeneity on Plant Cell Culture Performance

Interest of cellular differentiation in the production of vincristine and vinblastine in suspension cultures of Catharanthus roseus (L.) G Don.

“…These differences in gas composition and inoculum used for the bioreactor cultures explained difference performance, though the same PGR regimen was used for both bioreactor cultures. Any cell culture made will be unique due to its physiological state, record of subculture, and inherent heterogeneity (somoclonal variation, aggregates sizes, among others) (Bhatia & Sharma, 2015;Deus-Neumann & Zenk, 1984;Patil & Roberts, 2013). The findings reported here are in line with this.…”

A flow cytometric method for estimating S-phase duration in plants

“…Cells in the interior of aggregates are subject to a different microenvironment compared with free floating cells or cells on the surface of aggregates (Street, 1973), including differences in oxygen (Hulst et al , 1989) and nutrient availability (Patil and Roberts, 2013). Other factors that may contribute to the difference in S-phase duration include genetic and epigenetic instability, such as chromosome rearrangements, DNA methylation, and somatic mutation (reviewed by Lee and Phillips, 1988; Phillips et al , 1994; Tanurdzic et al , 2008).…”