Bioprocess Engineering of Plant Cell Suspension Cultures

Andrews, Gregory R.; Roberts, Susan C.

doi:10.1002/9783527800599.ch10

Cited by 4 publications

(4 citation statements)

References 177 publications

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“…Although these experiments were conducted in shake‐flasks, these concepts and data could be applied at any scale. Bioreactors often provide movement to cultures through employment of shaking, propellers, or rotation for aeration, which are also sources of mechanical shear . The developed PBE model could readily be applied at a production scale simply by continuously pumping culture through a tube with an optimized aperture size at an optimized flow rate.…”

Section: Discussionmentioning

confidence: 99%

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A population balance model to modulate shear for the control of aggregation in Taxus suspension cultures

Wilson

Maindarkar

McKee

et al. 2019

Biotechnology Progress

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Cellular aggregation in plant suspension cultures directly affects the accumulation of high value products, such as paclitaxel from Taxus. Through application of mechanical shear by repeated, manual pipetting through a 10 ml pipet with a 1.6 mm aperture, the mean aggregate size of a Taxus culture can be reduced without affecting culture growth. When a constant level of mechanical shear was applied over eight generations, the sheared population was maintained at a mean aggregate diameter 194 μm lower than the unsheared control, but the mean aggregate size fluctuated by over 600 μm, indicating unpredictable culture variability. A population balance model was developed to interpret and predict disaggregation dynamics under mechanical shear. Adjustable parameters involved in the breakage frequency function of the population balance model were estimated by nonlinear optimization from experimentally measured size distributions. The optimized model predictions were in strong agreement with measured size distributions. The model was then used to determine the shear requirements to successfully reach a target aggregate size distribution. This model will be utilized in the future to maintain a culture with a constant size distribution with the goal of decreasing culture variability and increasing paclitaxel yields.

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Section: Discussionmentioning

confidence: 99%

“…Bioreactors often provide movement to cultures through employment of shaking, propellers, or rotation for aeration, which are also sources of mechanical shear. 36,37 The devel-…”

Section: Using the Model To Create Optimized Culture Aggregation Prmentioning

confidence: 99%

A population balance model to modulate shear for the control of aggregation in Taxus suspension cultures

Wilson

Maindarkar

McKee

et al. 2019

Biotechnology Progress

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“…A plant cell system is a promising recombinant protein production platform owing to the capacity for post-translational modification, as well as the low production cost. In addition, a plant cell system faces little or no risk of human pathogen contamination, and therefore, offers a high level of biosafety compared with current commercial mammalian and microbial host cells [ 22 ]. Rice is a low-allergen staple food and a model research plant.…”

Section: Introductionmentioning

confidence: 99%

Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells

Huang

Sinaga

Tan

et al. 2021

IJMS

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The rice cell suspension culture system is a good way to produce recombinant human proteins, owing to its high biosafety and low production cost. Human Octamer-binding Transcription Factor 4 (Oct4) is a fundamental transcription factor responsible for maintaining human pluripotent embryonic stem cells. Recombinant Oct4 protein has been used to induce pluripotent stem cells. In this study, recombinant Oct4 proteins are produced via a sugar starvation-inducible αAmy3/RAmy3D promoter–signal peptide-based rice recombinant protein expression system. Oct4 mRNAs accumulate in the transgenic rice suspension cells under sugar starvation. The Oct4 recombinant protein is detected in the transgenic rice suspension cells, and its highest yield is approximately 0.41% of total cellular soluble proteins after one day of sugar starvation. The rice cell-synthesized recombinant human Oct4 protein show DNA-binding activity in vitro, which implies that the protein structure is correct for enabling specific binding to the target DNA motif.

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“…Clinical trial EGCG dosing is based 10 mg/kg body weight, which can be achieved from green tea [18]. Pharmaceuticals may require enormous amounts of the biologic source: 1,520 Taxus chinensis (Chinese yew) trees, an endangered species, are used to produce 1 kg of paclitaxel [19]. Therefore, pharmaceuticals may require partially or totally synthetic manufacturing processes including plant cell suspension cultures to preclude extinction of their natural sources [19].…”

Section: Nutraceuticalsmentioning

confidence: 99%

Nutraceuticals: Curative Integrative Cancer Treatment

Nwanodi¹

2017

Altern Integr Med

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Statement of Purpose: Although 75% of breast cancer patients may use integrative medicine, only 11.5% earlystage breast cancer patients believe integrative medicine has anticancer activity. The gap in users of and firm believers in integrative medicine indicates a need to increase awareness of integrative medicine's applicability to curative cancer treatment. As diet, nutraceuticals, and traditional Chinese medicine are used by up to 82% of cancer patients who use integrative medicine, this paper focuses on nutraceuticals. As female cancer patients are most likely to use integrative medicine, nutraceuticals specific to breast, cervical, endometrial, and ovarian cancer are reviewed.Methods: PubMed searches in September 2016 and January 2017, and accompanying hand searches were performed for English language, free full text articles published from 2012 onwards. Search terms were combinations of the key words: Homeopathy, phytochemicals, breast cancer, cervical cancer, endometrial cancer, ovarian cancer, cancer, prevention, treatment. Curative nutraceutical treatments were taken from these searches. Supplemental hand searches were performed as needed. Findings:Integrative nutraceutical therapies are based on biologic plausibility. Individual nutraceuticals are frequently comprised of numerous phytochemical types, and have multiple mechanisms of action. Agonistantagonist mechanisms are not exclusive to phytoestrogens, complicating which derivative or whole nutraceutical to use. Research on breast and cervical cancer inhibitors has seemingly outpaced that for endometrial and ovarian cancer. Breast cancer inhibitors include terpenes, isoflavones, organosulphurs, organoselenium compounds, and withanolides. Conclusion and Significance:Potential nutraceutical candidates for curative cancer treatment abound. Discerning which constituents, which extraction method, and which delivery method to use for an efficacious treatment is an essential, lengthy process. If nutraceuticals such as limonene and Kahalalide F move forward to Phase III trials a nutraceutical cancer treatment pipeline may be established. Withaferin-A and derivatives for breast and ovarian cancer appear to be the leading gynecologic cancer nutraceutical drug candidates.

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Bioprocess Engineering of Plant Cell Suspension Cultures

Cited by 4 publications

References 177 publications

A population balance model to modulate shear for the control of aggregation in Taxus suspension cultures

A population balance model to modulate shear for the control of aggregation in Taxus suspension cultures

Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells

Nutraceuticals: Curative Integrative Cancer Treatment

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