Implementation of a dose–response curve for γ-radiation in the Portuguese population by use of the chromosomal aberration assay

Martins, Vanda; Antunes, Ana; Gil, Octávia Monteiro

doi:10.1016/j.mrgentox.2012.09.009

Cited by 20 publications

(20 citation statements)

References 28 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The above calculation of u showed that the dicentric followed a Poisson distribution patterns (u ≥ ± 1.96) at the low dose whereas at the dose 0,5 -4 Gy follow non Poisson distribution (u ≤ ± 1.96). The similar result also found in research conducted by Martin et al whereas the dicentric distribution were consistent with Poisson at the lower doses but were over dispersed at the higher doses (1-3 Gy) [18]. This result is likely influenced by the sensitivity of each chromosome.…”

Section: Resultssupporting

confidence: 89%

Dose-Response Curve of Chromosome Aberrations in Human Lymphocytes Induced by Gamma-Rays

et al. 2014

View full text Add to dashboard Cite

Chromosome aberration is a biomarker to predict the level of cell damage caused by exposure to ionizing radiation on human body. Dicentric chromosome is a specific chromosome aberration caused by ionizing radiation and is used as a gold standard biodosimetry of individuals over exposed to ionizing radiation. In radiation accident the dicentric assays has been applied as biological dosimetry to estimate radiation absorbed dose and also to confirm the radiation dose received to radiation workers.The purpose of this study was to generate a dose response curve of chromosome aberration (dicentric) in human lymphocyte induced by gamma radiation. Peripheral blood samples from three non smoking healthy volunteers aged between 25-48 years old with informed consent were irradiated with dose between 0.1-4.0 Gy and a control using gamma teletherapy source. The culture procedure was conducted following the IAEA standard procedures with slight modifications. Analysis of dose-response curves used was LQ model Y = a + αD + βD 2. The result showed that α and β values of the curve obtained were 0.018 ± 0.006 and 0.013 ± 0.002, respectively. Dose response calibration curve for dicentric chromosome aberrations in human lymphocytes induced by gamma-radiation fitted to linear quadratic model. In order to apply the dose response curve of chromosome aberration disentric for biodosimetry, this standar curve still need to be validated.

show abstract

Section: Resultssupporting

confidence: 89%

Dose-Response Curve of Chromosome Aberrations in Human Lymphocytes Induced by Gamma-Rays

et al. 2014

View full text Add to dashboard Cite

show abstract

“…However, until recently, the construction of curves based on individual variability of dicentric chromosomes have not been well studied. Martins et al (2013) produced calibration curves based on data from sixteen donors. The donors were distributed by age and gender to examine potential effects on any differences observed.…”

Section: Discussionmentioning

confidence: 99%

“…We also emphasized that the dose rate was an important factor in dose estimations. Thus, intercomparison exercises should take into account the dose rates of the participating laboratories, because the dose rates might explain why Curve with low dose rate 7 Bauchinger et al (1983) versus Köksal et al (1995) Bauchinger (2002) versus Köksal et al (1995) Schmid et al (2002 versus Lloyd et al (1986) This work versus Köksal et al (1995) This work versus Lloyd et al (1986) Martins et al (2013) versus Köksal et al (1995) Martins et al (2013 versus Lloyd et al (1986) Dose rate (Gy/min)/0.5 Gy/min 3% -91%…”

Section: Discussionmentioning

confidence: 99%

Calibration curves by 60Co with low dose rate are different in terms of dose estimation – a comparative study

et al. 2020

View full text Add to dashboard Cite

Biological dosimetry aims to estimate individual absorbed doses due ionizing radiation exposure. The dicentric chromosomes are considered the most specific biomarker for dose estimation. This study aimed to compare calibration curves for linear low energy transfer (LET) radiation built from low dose rates and whether they vary in terms of dose estimation. For that we did a search in the literature of all calibration curves produced with low dose rates and we simulated the dose estimation from pre-established dicentric's frequencies. The information on methodologies and cytogenetic results of each study were analyzed. As expected dose rate influence b coefficients, especially at higher doses. However, we have seen that some doses were not statistically different but they should be, because there is a significant association between the productions of dicentrics and dose rate. This comparative study reinforced the robustness of the dicentric assay and its importance in biological dosimetry. We also emphasized that the dose rate was an important factor in dose estimations. Thus, intercomparison exercises should take into account the dose rates of the participating laboratories, because the dose rates might explain why some results of estimated doses fall outside the recommendations.

show abstract

“…However, dicentrics and MN decline over time and are not appropriate for dose estimation several months after an exposure. Stable cytogenetic endpoints persist longer and have been used successfully in the follow-up study of a radiation accident [2,3].…”

Section: Introductionmentioning

confidence: 99%

Eight-year follow-up study of three individuals accidentally exposed to 60Co radiation: Chromosome aberration and micronucleus analysis

Wang

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

View full text Add to dashboard Cite

Implementation of a dose–response curve for γ-radiation in the Portuguese population by use of the chromosomal aberration assay

Cited by 20 publications

References 28 publications

Dose-Response Curve of Chromosome Aberrations in Human Lymphocytes Induced by Gamma-Rays

Dose-Response Curve of Chromosome Aberrations in Human Lymphocytes Induced by Gamma-Rays

Calibration curves by 60Co with low dose rate are different in terms of dose estimation – a comparative study

Eight-year follow-up study of three individuals accidentally exposed to 60Co radiation: Chromosome aberration and micronucleus analysis

Contact Info

Product

Resources

About