Dose-Response Curve of Chromosome Aberrations in Human Lymphocytes Induced by Gamma-Rays

Lusiyanti, Yanti; Alatas, Zubaidah; Lubis, Masnelli; Suvifan, Viria Agesti; Ramadhani, Dwi; Purnami, Sofiati

doi:10.17146/aij.2013.254

Cited by 8 publications

(10 citation statements)

References 14 publications

(20 reference statements)

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“…Biological dosimetry (biodosimetry) is a method to determine the absorbed dose in individuals with unplanned exposure to ionizing radiation when the physical dosimeter was unavailable. [12] Biodosimetry using chromosome aberration biomarker has been a valuable dose assessment method, especially when there are difficulties in interpreting the data given by physical dosimetry or in case of radiation overexposure. The dicentric chromosome assay (DCA) was the only available biodosimetry method for many years, either after accidental or occupational exposure.…”

Section: Introductionmentioning

confidence: 99%

Development of Dose-Response Calibration Curve for Dicentric Chromosome Induced by X-Rays

et al. 2019

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Chromosome aberration is a biomarker that has been used as a standard tool in biological dosimetry (biodosimetry) of individuals after exposure to ionizing radiation. It is based mainly on the induction of dicentric chromosomes – one of the radiation-induced biological effects, in order to correlate them with radiation dose. In this study, a dose calibration curve for X-rays was generated by using the dicentric assay and by fitting the data to both Chromosomal Aberration Calculation Software and Dose Estimate programs to compare the output of each method. Peripheral blood samples from four nonsmoker healthy donors were irradiated with various doses ranging from 0 to 4 Gy with 250 kV or 122 keV X-rays at a dose rate of 0.17 Gy/min. The irradiated blood was cultured, harvested, and analyzed according to the standard procedure as described by the International Atomic Energy Agency with slight modifications. The dose-response calibration data for dicentrics were fitted with the linear-quadratic model (Y dic = 0.03987D 2 + 0.00651D). The dose-response calibration curve obtained in this research was comparable to other estimations with similar radiation quality and dose rates. The results in this research convinced us in sustaining a biodosimetry using a dose-response calibration curve in our laboratory.

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Section: Introductionmentioning

confidence: 99%

Development of Dose-Response Calibration Curve for Dicentric Chromosome Induced by X-Rays

et al. 2019

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“…The CBMN assay is established as a very reliable, thoroughly validated and is a standardized technique in the field of radiation biology to assess in vivo radiation exposure of occupational, medical, and accidentally exposed individuals and to determine individual in vitro radiosensitivity or cancer susceptibility. [ 2 3 4 ] To improve our cytogenetic laboratory capability as the National Cytogenetic Biodosimetry Laboratory in Indonesia, we have established our own dose-response standard curves of unstable chromosome aberrations for gamma radiation[ 5 ] and X-rays. [ 6 ]…”

Section: Introductionmentioning

confidence: 99%

“…[ 2 3 4 ] To improve our cytogenetic laboratory capability as the National Cytogenetic Biodosimetry Laboratory in Indonesia, we have established our own dose-response standard curves of unstable chromosome aberrations for gamma radiation[ 5 ] and X-rays. [ 6 ]…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Dose-response Curve for X-ray-Induced Micronuclei in Human Lymphocytes

et al. 2016

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The cytokinesis-block micronucleus assay in peripheral blood lymphocytes is an established technique for biodosimetry. The aim of this project was to generate a X-ray induced micronuclei (MN) curve for peripheral blood lymphocytes taken from five healthy donors. The blood samples were irradiated with X-rays of 122 KeV at a dose rate of 0.652 Gy/min to doses of 0.5, 1, 2, 3, and 4 Gy. The blood samples were then cultured for 72 h at 37°C and processed following the International Atomic Energy Agency standard procedure with slight modifications. The result showed that the yields of MN frequencies were increased with the increase of radiation dose. Reconstruction of the relationship of MN with dose was fitted to a linear-quadratic model using Chromosome Aberration Calculation Software version 2.0. Due to their advantages, mainly, the dependence on radiation dose and dose rate, despite their limitation, these curves will be useful as alternative method for in vitro dose reconstruction and can support the preparedness for public or occupational radiation overexposure and protection. The results reported here also give us confidence to apply the obtained calibration curve of MN for future biological dosimetry requirements in Indonesia.

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“…From the results of this study, it was clear that the radiation dose-response calibration curve for the MN analysis should be produced in the same laboratory. The laboratory previously successfully developed a dose-response calibration curve for dicentric analysis induced by gamma rays [15]. In order to establish a biodosimetry using MN analysis, the dose-response calibration curve for MN analysis must be constructed first.…”

Section: Resultsmentioning

confidence: 99%

Biological Dosimetry Using Micronucleus Assay in Simulated Partial-Body Exposure to Ionizing Radiation

et al. 2017

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In radiation accidents, it is common that only several parts of the body are exposed to radiation. As a consequence there is a mixture of exposed and unexposed lymphocytes in peripheral blood cells of the samples. This phenomenon will cause the dose value estimated using the exposed lymphocytes to be lower than the actual dose. In this study, an assessment of partial body exposures using micronucleus assay by estimating the partial body dose and fraction of irradiated blood was conducted. An optimal D 0 value also has been determined in this study to estimate the fraction of irradiated cells. Peripheral blood lymphocytes (PBLs) from three healthy donors were irradiated in vitro with 2 Gy of X-rays. Partial radiation exposure was simulated by mixing the irradiated and non-irradiated blood in different proportions. The proportions of mixtures of blood samples irradiated in vitro were 5, 10, 15, 20, and 30 %. Blood samples were then cultured and harvested based on micronuclei assay protocol. At least 2000 binucleated cells with wellpreserved cytoplasm were scored for the MN frequency. Dose Estimate 5.1 software was used to calculate the dispersion index (σ 2 /y) and normalized unit of this index (U) in each proportion of bloods. The fractions of irradiated cells were calculated with CABAS (Chromosomal Aberration Calculation Software) for several different D 0 values (2.7; 3.8; 5.4). The results showed that D 0 value at 5.4 gave the closest results to the actual proportion of irradiated bloods, while for the dose estimation the estimated doses value from all proportions in all donors were higher than the actual dose. The factor that may cause this phenomenon was that the dose response calibration curve used to predict the radiation dose was not constructed in the laboratory used. Overall it can be concluded that a biodosimetry using MN assay can be used to estimate the radiation dose in partial body exposure. In order to establish a biodosimetry using MN analysis the dose-response calibration curve MN analysis should be constructed first in the laboratory used.

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Dose-Response Curve of Chromosome Aberrations in Human Lymphocytes Induced by Gamma-Rays

Cited by 8 publications

References 14 publications

Development of Dose-Response Calibration Curve for Dicentric Chromosome Induced by X-Rays

Development of Dose-Response Calibration Curve for Dicentric Chromosome Induced by X-Rays

Establishment of a Dose-response Curve for X-ray-Induced Micronuclei in Human Lymphocytes

Biological Dosimetry Using Micronucleus Assay in Simulated Partial-Body Exposure to Ionizing Radiation

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