Impedimetric Biosensor for the Detection of Bacterial Lipopolysaccharide Based on Lectin-functionalized Gold Nanoparticle?Graphene Composites

Abstract

We developed a simple impedimet ric biosensor for the detection of bacterial lipopolysaccharide (LPS) based on lectin-functionalized gold nanoparticle (AuNP)-graphene (G) composites. The effects of AuNPs on G were investigated by comparison with the sensing surface in the absence of AuNPs. The results showed that the AuNPs formed a 3D micro/ nanocomposite on G, which increased the electroactive area from 7.03 × 10 −2 to 1.41 × 10 −1 cm 2 , and also increased the heterogeneous electron transfer rate, which can improve the sensitivity of the biosensor. Concanavalin A (Con A) lectin was used as a molecular recognition element because of its specific interaction toward the carbohydrate groups in LPS. The increase in electron transfer resistance was in a logarithmically direct proportion to the LPS concentration and lowered the detection limit to 600 pg/L. The fabricated biosensor achieved the detection of LPS with good sensitivity, acceptable reproducibility, and stability. This method may hold promise for potential applications in glycomic biosensing.

Environmental protection based on the nanobiosensing of bacterial lipopolysaccharides (LPSs): material and method overview

“…Au and graphene nanoparticles have recently been recognized as efficient and most promising electrochemical and luminescence catalysts. 147 …”

“…Au and graphene nanoparticles have recently been recognized as efficient and most promising electrochemical and luminescence catalysts. 147 Fig. 7 Graphical illustration of the developed aptasensor for the detection of LPS.…”

Graphene biosensors for bacterial and viral pathogens

“…9 B) ( Rengaraj et al, 2018 ). Qin and colleagues constructed an impedimetric biosensor utilizing AuNPs–Graphene electrode modified with ConA for the sensitive detection of LPS on the bacterial outer membrane with the detection limit of 600 pg/L ( Qin et al, 2019 ). Moreover, glycans functionalized graphene-based biosensor has been reported for the diagnosing of human-infectious avian influenza virus infections ( Ono et al, 2017 ).…”