Impairment of male fertility induced by muscarinic receptor antagonists in rats

Ban, Yoshiki; Sato, Takahiro; Nakatsuka, Toshio; Kemi, Masayuki; Samura, Keiji; Matsumoto, Hajime; Cukierski, Mark A.; Zwieten, Matthew J. van

doi:10.1016/s0890-6238(02)00050-3

Cited by 15 publications

(13 citation statements)

References 29 publications

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“…Indeed, the importance of mAChR for fertility has been indicated by the findings that mAChR antagonists impair rat male fertility. However, the mechanisms that cause this infertility remain unclear (Ban et al 2002). Thus, knowledge of the expression and localization of mAChR subtypes in the organs of the male reproductive tract should facilitate our understanding of the role of the cholinergic nervous system in male (in)fertility.…”

Section: Discussionmentioning

confidence: 99%

“…Cholinergic agonists, such as methacholine and carbachol, induce epididymal contractions (Hib 1976;Laitinen and Talo 1981;Pholpramool and Triphrom 1984) by interacting with muscarinic acetylcholine receptors (mAChRs). mAChRs mediate most of the actions of the cholinergic neurotransmitter in the male rat reproductive tract (Miranda et al 1994(Miranda et al , 1995Pennefather et al 2000;Borges et al 2001;Ventura et al 2002;Ban et al 2002;Nadelhaft 2003). In mammals, five distinct mAChR subtypes (M 1 to M 5 ) have been identified, with each receptor being the product of a different gene (see Birdsall et al 1998;Caufield and Birdsall 1998;Eglen et al 1999Eglen et al , 2001van Koppen and Kaiser 2003).…”

Section: Introductionmentioning

confidence: 99%

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Expression and localization of muscarinic acetylcholine receptor subtypes in rat efferent ductules and epididymis

et al. 2005

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The expression of muscarinic acetylcholine receptor (mAChR) subtypes (M(1)-M(5)) was studied in the rat efferent ductules and epididymis at the mRNA and protein levels. The relative abundance of each mAChR transcript subtype differed depending on the tissue and the epididymal region analyzed. The M(1) mAChR mRNA level was more abundant in the efferent ductules than in the caput and cauda of the epididymis. The M(2) mAChR mRNA level was similar between the efferent ductules and caput of the epididymis and higher in the cauda region. The M(3) mAChR mRNA level was low in the efferent ductules and caput of the epididymis, but high levels were detected in the cauda region. mRNAs for M(4) and M(5) mAChRs were not detected in these tissues. Our studies indicated a variable degree of immunostaining for each mAChR subtype in a cell-type and tissue-specific pattern. M(1) mAChR was detected over the efferent ductule epithelium. M(2) and M(3) mAChRs were observed in the apical region of the ciliated cells. Apical and narrow cells of the initial segment showed distinct staining by M(1) antibody, whereas a supranuclear reaction was noted in the principal cells of the caput of the epididymis. In addition, staining for M(1) and M(2) mAChRs was visible in the apical membrane of some epithelial cells of the cauda region. M(3) mAChR was detected in the peritubular smooth muscle of the efferent ductules and epididymis. Functional studies suggested the involvement of this subtype in epididymal tubule contraction. Thus, the cell-specific expression of the various mAChR subtypes in the efferent ductules and epididymis suggests that these receptors play a role in the modulation of luminal fluid composition and smooth muscle contraction.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression and localization of muscarinic acetylcholine receptor subtypes in rat efferent ductules and epididymis

et al. 2005

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“…Most of the actions of ACh in the male reproductive tract are induced by its effects on mAChR (Miranda et al 1994(Miranda et al , 1995Pennefather et al 2000;Ban et al 2002;Ventura et al 2002;Nadelhaft 2003;Lucas et al 2008). …”

Section: Machr In the Male Reproductive Tractmentioning

confidence: 99%

“…In vivo treatment with mAChR antagonists has been reported to induce impairment of fertility in male rats, possibly due to inhibition of sperm and semen transport from the vas deferens and seminal vesicle to the urethra during emission (Ban et al 2002;Sato et al 2005). Also, the existence of binding sites for mAChR-specific agonists in mouse and rabbit sperm (Florman and Storey 1982;Young and Laing 1991) raises the hypothesis that, besides its effects on smooth muscle contraction, ACh may interfere with sperm function.…”

Section: Machr In the Male Reproductive Tractmentioning

confidence: 99%

Muscarinic Acetylcholine Receptor Subtypes in the Male Reproductive Tract

et al. 2009

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In mammals, at least five different muscarinic acetylcholine receptor subtypes (mAChRs; M(1)-M(5)) are known to be widely expressed and distributed in different tissues from different species. They mediate distinct physiological functions according to their location and receptor subtype. Multiple events are associated with the regulation of intracellular signaling by mAChRs, and a coordinated balance of the molecular mechanisms governing receptor signaling, desensitization, resensitization, and mitogenic signaling is known to occur in various cell types. Most of the actions of acetylcholine (ACh) in the male reproductive tract are induced by its effects on mAChRs, but the role of specific mAChR subtypes on male reproductive function and fertility are still not well understood. The rat efferent ductules and epididymis are androgen-dependent tissues of the male reproductive tract, with important roles in the process to form a viable and fertile sperm. In the present study, aspects of the expression, localization, and potential function of mAChR subtypes in rat efferent ductules and epididymis are reviewed. Furthermore, evidences for the implication of mAChRs in the regulation of protein synthesis and secretion in these tissues are presented. Taken together, the studies contribute to our understanding about physiological aspects of mAChR and mechanisms by which the cholinergic system affects male reproduction.

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“…Ban et al, (2002) y Sato et al, (2005 aplicaron 62,5 y 125 mg/kg/día de atropina en ratas machos por una semana, encontrando un deterioro en la fertilidad de los mismos, concluyendo que los antagonistas de los receptores muscarinicos como la atropina inhiben la contracción de los vasos deferentes, epidídimo y de la vesícula seminal en ratas, causando infertilidad.…”

Section: Introductionunclassified

Efecto de Brugmansia arborea (L.) Lagerheim (Solanacea) en el sistema reproductor masculino de ratón

Pino

Alvis

2008

Rev peru biol

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NOTA CIENTÍFICA ResumenEl objetivo del presente estudio fué investigar el efecto de la administracion del extracto acuoso de Brugmansia arborea (L.) Lagerheim -floripondio-sobre algunos parámetros reproductivos en mamíferos. Ratones machos fueron tratados con 70 mg/kl/pc por 7 días, después de lo cual fueron eutanizados determinando los pesos de testículo, epidídimo e individualmente su región caudal; además, se contabilizó la concentración y malformaciones espermáticas. El peso del testículo y la cola del epidídimo disminuyeron; la concentración espermática fue menor que el control, se incrementaron las malformaciones espermáticas. Estos resultados sugieren que existe un efecto negativo de la dosis de B. arborea que alteraría la fertilidad del ratón.Palabras Clave: Brugmansia arborea, floripondio, Datura, fertilidad. AbstractThe aim of this study was to investigate the effect of administration of aqueous extract of Brugmansia arborea (L.) Lagerheim-Angel's Trumpet-on some reproductive parameters in mammals.Male mices were treated with 70 mg/kl/cw for 7 days, where they were euthanized and its testis, epididymis and its cauda region weights where determinate; so, the sperms number and its abnormalities were accounted. The testis and epididymis tail weight fell; sperm concentration was lower than control and the abnormalities rises. These results suggest that there is a negative effect of the dose of B. arborea than would alter the fertility of the mouse.

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Impairment of male fertility induced by muscarinic receptor antagonists in rats

Cited by 15 publications

References 29 publications

Expression and localization of muscarinic acetylcholine receptor subtypes in rat efferent ductules and epididymis

Expression and localization of muscarinic acetylcholine receptor subtypes in rat efferent ductules and epididymis

Muscarinic Acetylcholine Receptor Subtypes in the Male Reproductive Tract

Efecto de Brugmansia arborea (L.) Lagerheim (Solanacea) en el sistema reproductor masculino de ratón

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