Impairment of Interleukin-17A Expression in Canine Visceral Leishmaniosis is Correlated with Reduced Interferon-γ and Inducible Nitric Oxide Synthase Expression

Nascimento, Manuela Sales Lima; Albuquerque, T.D.R.; Nascimento, Alvaro F.S.; Caldas, Ivo Santana; Do-Valle-Matta, Maria Adelaide; Souto, Janeusa Trindade de; Talvani, André; Bahia, Maria T.; Galvão, Lúcia Maria da Cunha; Câmara, Antônia Claudia Jácome da; Guedes, Paulo Marcos da Matta

doi:10.1016/j.jcpa.2015.10.174

Cited by 27 publications

(30 citation statements)

References 26 publications

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“…The high frequency of cells producing NO observed in the spleen of dogs with leishmaniasis confirmed previous results in which higher proportions of cells producing iNOS were observed in dogs with leishmaniasis [26,8] Conversely, the levels of NO were reduced in the spleen, suggesting that the leishmanicidal function is impaired, in fact symptomatic dogs showed lower iNOS mRNA expression than asymptomatic [27]. and high iNOS expression was observed in macrophages containing little or no amastigotes internalized in the liver samples, skin and lymph node of dogs with VL.…”

Section: Discussionsupporting

confidence: 88%

Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis

Martini

Andrade

Almeida

et al. 2018

Comparative Immunology, Microbiology and Infectious Diseases

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Nitric oxide (NO) is involved in the death of the Leishmania parasite and regulation of apoptosis. We quantified the frequency of cells producing NO and its levels in the peripheral blood mononuclear cells (PBMC), leukocytes from spleen in Visceral Leishmaniasis (VL) symptomatic dogs and correlated NO levels with apoptosis and parasite load in the spleen. The percentage of NO+ cells and CD14+/NO+ was higher in PBMC and spleen cells in infected dogs than in controls. The levels of NO+ and CD14+/NO+ cells was higher in PBMC, but lower spleen of dogs infected than compared to control. Late apoptosis rates increased in PBMC and spleen of infected dogs compared to controls, and the NO levels and apoptosis not showed correlation. There was a positive correlation between the percentage of cells producing NO in the spleen and parasite load. The NO participates in the immune response in the canine VL, but it is not apoptosis inducer.

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Section: Discussionsupporting

confidence: 88%

Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis

Martini

Andrade

Almeida

et al. 2018

Comparative Immunology, Microbiology and Infectious Diseases

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“…The authors concluded that the suppression of these receptors seen in symptomatic dogs was possibly due to a reduced migration of immune cells, which would normally assist with parasite clearance and granuloma formation (Nascimento et al 2013). Recently, the same authors documented that the progression of this disease was characterized not only by the down regulation of T helper (Th) 1-related cytokines, such as IFN- γ and TNF- α , but also by the down regulation of genes encoding interleukin (IL)-17A, inducible NO synthase (iNOS) and IL-10 in the spleen and liver in symptomatic dogs compared with asymptomatic dogs (Nascimento et al 2015). …”

Section: Organ Specific Immune Responsesmentioning

confidence: 99%

Insights on adaptive and innate immunity in canine leishmaniosis

2016

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SUMMARYCanine leishmaniosis (CanL) is caused by the parasite Leishmania infantum and is a systemic disease, which can present with variable clinical signs, and clinicopathological abnormalities. Clinical manifestations can range from subclinical infection to very severe systemic disease. Leishmaniosis is categorized as a neglected tropical disease and the complex immune responses associated with Leishmania species makes therapeutic treatments and vaccine development challenging for both dogs and humans. In this review, we summarize innate and adaptive immune responses associated with L. infantum infection in dogs, and we discuss the problems associated with the disease as well as potential solutions and the future direction of required research to help control the parasite.

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“…Recent studies show that Th17 cells act synergistically with the Th1 population to control L. infantum growth. The interleukin (IL)-17 A modulates some key regulatory cytokines including IL-10 and sustains IFN-γ production by Th1cells at sites of inflammation 5 . The active disease, however, has been linked to high antibody levels and a progressive Th2-deactivating immune response in the presence of a strong inflammatory reaction 6 .…”

Section: Introductionmentioning

confidence: 99%

Canine Leishmaniasis Progression is Associated with Vitamin D Deficiency

Rodríguez-Cortés

Martori

Martínez-Flórez

et al. 2017

Sci Rep

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The relationship between vitamin D deficiency and the risk of suffering from a plethora of health disorders, ranging from autoimmune processes to infectious diseases has been widely described. Nonetheless, the potential role of vitamin D in visceral leishmaniasis remains uncharacterized. In the Mediterranean basin, where the dog is leishmania’s main peri-domestic reservoir, control measures against the canine disease have shown beneficial effects on the incidence of human leishmaniasis. In this study, we measured the vitamin D levels in serum samples from a cohort of 68 healthy and disease dogs from a highly endemic area and we have also studied the relationship of these levels with parasitological and immunological parameters. The sick dogs presented significantly lower (P < 0.001) vitamin D levels (19.6 ng/mL) than their non-infected (31.8 ng/mL) and the asymptomatic counterparts (29.6 ng/mL). In addition, vitamin D deficiency correlated with several parameters linked to leishmaniasis progression. However, there was no correlation between vitamin D levels and the Leishmania-specific cellular immune response. Moreover, both the leishmanin skin test and the IFN-γ levels displayed negative correlations with serological, parasitological and clinical signs. Further studies to determine the functional role of vitamin D on the progression and control of canine leishmaniasis are needed.

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Impairment of Interleukin-17A Expression in Canine Visceral Leishmaniosis is Correlated with Reduced Interferon-γ and Inducible Nitric Oxide Synthase Expression

Cited by 27 publications

References 26 publications

Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis

Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis

Insights on adaptive and innate immunity in canine leishmaniosis

Canine Leishmaniasis Progression is Associated with Vitamin D Deficiency

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