Impacto Do Herpesvírus Bovino 1 E Do Vírus Da Diarréia Viral Bovina Na Transferência De Embriões

Rufino, F.A.; Seneda, Marcelo Marcondes; Alfieri, A.A.

doi:10.5380/avs.v11i1.5606

Cited by 5 publications

(5 citation statements)

References 15 publications

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“…The great advantage of this technique is that it is based on the identification of the viral genome; therefore, it does not require the presence of viable viral particles (HAYDEN et al, 1991;BÉLAK;BALLAGI-PORDÁNY, 1993). A nonlethal method to evaluate the presence of pathogens in embryos before their transfer to receivers is currently not available (RUFINO et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Natural infection in ovarian structures by bovine herpesvirus 1: molecular and serological detection

et al. 2015

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In this study, the polymerase chain reaction (PCR) was used to evaluate the presence of viral DNA in ovarian tissue, in the cumulus-oocyte complex (COC), follicular liquid, and blood of animals naturally infected with bovine herpesvirus-1 (BoHV-1). The serum profile of the sampled animals was also evaluated. Samples of serum, blood, ovarian tissue, follicular liquid, and COC were collected from 147 slaughterhouse animals that were not vaccinated against BoHV-1. Contaminated or insufficient samples were disregarded. Serological tests allowed the identification of serum-positive animals with neutralizing antibodies against BoHV-1. Analysis of samples by PCR revealed the presence of viral DNA in 0.9% (1/115) of the COC samples, in 4.3% (5/117) of the ovarian tissue samples, and in 2.8% (3/108) of the blood samples. Viral DNA was not detected in any of the follicular liquid samples. In serological samples, a positivity of 83.6% (117/140) was observed for BoHV-1. All PCR-positive animals, regardless of the samples analyzed, showed positivity in the serum neutralization test for the detection of BoHV-1-specific antibodies. According to these results, a high prevalence of antibodies against BoHV-1 was detected in naturally infected animals from different herds, and the molecular tests revealed the presence of viral DNA in bovine ovarian tissue, providing evidence that this might be a site of BoHV-1 infection in naturally infected animals. Key words: Bovine herpesvirus, infectious bovine rhinotracheitis, cumulus-oocyte complex, ovarian tissue ResumoNeste trabalho foi avaliada a presença do DNA viral, por meio da Reação em Cadeia da Polimerase (PCR), no tecido ovariano, nos oócitos, líquido folicular e sangue de vacas naturalmente infectadas. Também foi avaliado o perfil sorológico dos animais amostrados. Foram coletadas amostras de soro, sangue, tecido ovariano, líquido folicular e complexo cumulus-oócitos de 147 animais abatidos em frigorífico não vacinados contra o herpesvirus bovino 1 (BoHV-1). Amostras tóxicas ou insuficientes foram descartadas. Os testes sorológicos foram realizados permitindo a identificação dos animais soropositivos para anticorpos neutralizantes contra o BoHV-1. Foram realizadas as PCRs onde foi observada a presença do DNA viral em 0,9% (1/115) dos oócitos, em 4,3% (5/117) do tecido ovariano e em 2,8% (3/108) do sangue. Em nenhuma das amostras de líquido folicular foi detectado o DNA viral. Nas amostras sorológicas observou-se 83,6% (117/140) de positividade para o BoHV-1. Dentre 1 Discente, Universidade Federal de Viçosa, UFV, Viçosa, MG, Brasil. E-mail: emiliovet2004@yahoo.com.br 2 Profs., UFV, Viçosa, MG, Brasil. E-mail: epcosta@ufv.br; asjvet@hotmail.com 3 Discentes, UFV, Viçosa, MG, Brasil. E-mail: vramendes@hotmail.com; sanelylc@hotmail.com; marcusrsantos@yahoo.com os animais positivos na PCR, independente das amostras, todos apresentavam positividade no teste de soroneutralização para detecção de anticorpos para BoHV-1. Conclui-se que em animais de diferentes rebanhos analisados f...

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Section: Introductionmentioning

confidence: 99%

Natural infection in ovarian structures by bovine herpesvirus 1: molecular and serological detection

et al. 2015

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“…In some situations, the infection might be present in a latent form, and a seropositive animal might not present any clinical signs (Reinhold et al, 2007). In Brazil, reproductive failures represent the main sanitary problem in cattle herds (Takiuchi et al, 2005;Cortez et al, 2006aCortez et al, , 2006bRufino et al, 2006;Pilz el al., 2007;Dias et al, 2008). Despite being endemic in Brazilian beef and dairy cattle, Leptospirosis, Infectious Bovine Rhinotracheitis (IBR), and the Bovine Viral Diarrhea (BVD) can be controlled through vaccination programs .…”

Section: Resultsmentioning

confidence: 99%

Investigation of the possible role of Chlamydophila abortus in reproductive failures in nrazilian herds of domestic ruminants

Silva-Zacarias

Alfieri

Spohr

et al. 2009

Braz. arch. biol. technol.

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“…These improvements include animal breeding biotechnology, such as protocols for timed artificial insemination (TAI), superovulation of donors for collection and transfer of embryos (SOV/TE), in vitro embryos production (IVEP), cryopreservation of gametes and embryos and, also, sexed semen (Rufino et al, 2006;Vieira, 2012).…”

Section: Biotechnology Of Reproduction: Improving the Reproductive Efficiencymentioning

confidence: 99%

The impact of sanity on the fertility of cows submitted to reproductive biotechnics

Zangirólamo¹,

Silva²,

Morotti³

et al. 2017

Rev. Acad. Ciên. Anim.

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The productivity in the herds is directly associated with reproduction. Thus, in order to achieve a better efficiency, the most varied reproductive biotechniques have been applied to the plants. Several factors have a direct influence on reproductive performance, including genetics, nutrition, zootechnical management, and sanitation. However, the sanity of the herd through the use of prophylactic measures against infectious agents is often neglected. In this context, particular attention should be paid to health programs, since 37-50% of gestational losses in the herd are consequences of infectious diseases of the reproductive tract caused by viral, bacterial or protozoan agents. Thus, this review will discuss the main advantages obtained with the use of reproductive biotechniques to increase fertility and the epidemiological aspects related to the diseases that cause reproductive failures in cows. Finally, we will also explain the importance of implementing sanitary programs with adequate management and vaccination of animals, to minimize economic losses related to reproductive diseases and, consequently, obtain higher pregnancy rates in the use of reproduction biotechnologies.

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Impacto Do Herpesvírus Bovino 1 E Do Vírus Da Diarréia Viral Bovina Na Transferência De Embriões

Cited by 5 publications

References 15 publications

Natural infection in ovarian structures by bovine herpesvirus 1: molecular and serological detection

Natural infection in ovarian structures by bovine herpesvirus 1: molecular and serological detection

Investigation of the possible role of Chlamydophila abortus in reproductive failures in nrazilian herds of domestic ruminants

The impact of sanity on the fertility of cows submitted to reproductive biotechnics

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