Glyphosate and paraquat are effective preplant burndown herbicide options for multi-cropped vegetable production on plastic mulch, but problematic weeds such as wild radish, cutleaf eveningprimrose, annual morningglory, or horseweed may not be adequately controlled with these herbicides alone. 2,4-D and dicamba could help control these troublesome weeds prior to planting if they can be removed from plastic mulch for avoiding crop damage. Treatments included 2,4-D (1,065 and 2,130 g ae ha−1) and dicamba (560 and 1,120 g ae ha−1) applied broadcast over plastic mulch a day before transplanting. Just before transplanting, treatments received overhead irrigation at 0.76 cm or no irrigation. Plastic mulch samples were collected at application and planting to determine herbicide presence using analytical techniques, and cantaloupe and zucchini squash were subsequently transplanted on the plastic beds. Analytical UHPLC analysis determined 88-99% of the initial herbicide concentration was present at crop planting when irrigation was not implemented. At most, a 1/50 rate of dicamba and a 1/500 rate of 2,4-D was present at planting when overhead irrigation was applied prior to transplanting. Maximum cantaloupe and squash injury from 2,4-D with irrigation was 10% and did not influence plant growth, biomass, or yield. For dicamba with overhead irrigation, cantaloupe injury reached 35%, vine lengths were reduced 24% and maturity was delayed while squash injury ranged from 9 to 12% without influencing growth or yield. Without irrigation to wash herbicides from the mulch prior to planting, 60 to 100% injury of both crops occurred with both herbicides. Zucchini squash was more tolerant to dicamba compared to cantaloupe. Results demonstrated that 2,4-D can be adequately removed from the surface of plastic mulch with irrigation while a single irrigation event was not sufficient to remove dicamba.